High frequency somatic embryogenesis and plant regeneration from zygotic embryo-derived callus cultures of three Allium species

Abstract

High frequency somatic embryogenesis and plant regeneration from zygotic embryo-derived callus cultures of three Allium species Valk, P. van der; Scholten, O.E.; Verstappen, F.; Jansen, Ritsert; Dons, J.J.M. Take-down policy If you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediately and investigate your claim.Downloaded from the University of Groningen/UMCG research database (Pure): http://www.rug.nl/research/portal. For technical reasons the number of authors shown on this cover page is limited to 10 maximum. The plant regeneration ability of zygotic embryo-derived callus cultures was studied for 12 A. cepa varieties and accessions, two A. fistulosum varieties, one A. fistulosum x A. cepa interspecific hybrid and two A. porrum varieties. Compact embryogenic callus was induced on Murashige and Skoog (MS) medium supplemented with 2,4-dichlorophenoxyacetic acid. The embryogenic calluses of all three Allium species were similar in appearance. For all accessions tested plants could be regenerated at a high frequency from this compact callus through somatic embryogenesis, when using kinetin supplemented MS medium (regeneration medium). Addition of abscisic acid to the regeneration medium stimulated the formation of both somatic embryos and shoots for a number of varieties. Concerning shoot regeneration from callus cultures, significant differences existed between genotypes of all accessions except one.Abbreviations: 2,4-D-2,4-dichlorophenoxyacetic acid, VDH-Van Der Have Seed company

Agronomic and Biotechnological Strategies for Breeding Cultivated Garlic in Mexico

“…Protocorm and protocorm-like structures were both light and dark-green colored, compact and easily detachable (Fig. 7a, b and c), somehow similar to organogenic callus from A. cepa (Van der Valk et al, 1992;Zheng et al, 1998). 'Chino' and 'Coreano' protocorms were even more easily detached, dark-green colored as compared to 'Perla' derived genotypes; Novák et al, (1986) also found differential pigmentation among genotypes.…”

Chromosomal Variation in Callus Lines and Regenerated Plantlets from Three Cultivars of Allium fistulosum L. (2n=16).

“…Studies on plant regeneration from A. fistulosum callus cells have been reported by several authors ( Van der Valk et al 1992, Song and Peffley 1994, Kim and Soh 1996. These reports concerned plant regeneration occurring through somatic embryogenesis, although detailed descriptions of the process of embryogenesis were not provided.…”

Organogennsis and Somatic Embryogenesis from Young Flower Buds of Agapanthus africanus Hoffmanns.

“…monocotyledonous plants including Liliaceous plants, 2, 4-D was predominantly used for the induction of embryogenic callus [3,9]. In Agapanthus africanus, however, somatic embryogenesis was not induced by 2, 4-D but by picloram.…”