High-Frequency Ultrasound Imaging to Evaluate Liver Fibrosis Progression in Rats and Yi Guan Jian Herbal Therapeutic Effects

Chen, Wei; Chen, Jiun Yu; Tung, Yu Tang; Chen, Hsiao Ling; Kuo, Chia Wen; Chuang, Chia Hui; Chong, Kowit-Yu; Mao, Frank Chiahung; Chen, Chuan-Mu

doi:10.1155/2013/302325

Cited by 17 publications

(11 citation statements)

References 36 publications

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“…After anesthesia, the abdominal area was shaved and cleaned. Ultrasound exams were performed using L40‐8/12, linear transducer of 20 MHz lLongitudinal evaluation of each rat's abdomen was blindly scanned on day 0, 7, 14, and 21, and portal vein caliber (in mm) was determined …”

Section: Methodsmentioning

confidence: 99%

Periodontal disease may induce liver fibrosis in an experimental study on Wistar rats

Meşter

Ciobanu

Taulescu

et al. 2019

Journal of Periodontology

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Background The aim was to assess the effects of periodontal disease in promoting liver fibrosis in a rat model of ligature‐induced periodontitis. Methods Twenty‐four Wistar rats were divided into four groups: control (CTRL), experimental periodontitis group at day 7 (PER7), at day 14 (PER14), at day 21 (PER21). Experimental periodontitis was induced by the placement of a silk ligature around mandibular incisors. The following parameters were assessed: gingival index, tooth mobility; liver status, and portal vein caliber by ultrasound examination; bone retraction, bone mineral density (BMD), bone volume/tissue volume (BV/TV) by micro‐CT analysis; aspartate aminotransferase (ASAT) and alanine aminotransferase (ALAT); oxidative stress (malondialdehyde [MDA], reduced glutathione/oxidative glutathione ratio [GSH/GSSG]), and matrix metalloproteinase‐8 (MMP‐8) levels; and histopathological evaluation of periodontal and liver tissues. Results Periodontal parameters showed the development of periodontitis in experimental groups. Micro‐CT results indicates an increase of bone retraction and BMD values and a decrease of BV/TV value in PER groups. Liver fibrosis could not be diagnosed with ultrasound examination in any of the groups. Elevated levels of ASAT and ALAT in PER groups compared with CTRL group were found. MDA have indicated elevated levels and a decrease of GSH/GSSG ratio in PER group compared with the CTRL group. Levels of MMP‐8 have indicated high values in PER21 compared with the other groups. Histological analysis of the periodontal and liver tissues sustains the link between periodontal and hepatic injury. Conclusion This study demonstrates a positive correlation between periodontal lesions and liver disease. Periodontitis may be an independent risk factor for liver fibrosis.

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Section: Methodsmentioning

confidence: 99%

Periodontal disease may induce liver fibrosis in an experimental study on Wistar rats

Meşter

Ciobanu

Taulescu

et al. 2019

Journal of Periodontology

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“…On the other hand, the qualitative scoring system applied allowed to find significant differences between the different types of infections. It should be considered that both the quantitative and qualitative applied in our study have been extrapolated from numerous pre-clinical and clinical reports (Aubé et al, 1999; Mathiesen et al, 2002; Lu et al, 2003; Bonekamp et al, 2009; Lessa et al, 2010; Resende et al, 2011; Chen et al, 2013). Since this is the first application in a murine model of bacterial infection, the lack of significance might be more related to the need of a diversified diagnostic approach between different species than to the absence of pathologic ultrasonographic features.…”

Section: Discussionmentioning

confidence: 99%

“…Recently, transient and real-time elastography are being used to assess more objectively and accurately tissue elasticity but they showed to be reliable only for extensive fibrosis or cirrhosis (Bonekamp et al, 2009; Gerstenmaier and Gibson, 2014; Heller and Tublin, 2014). Most pre-clinical studies focused on steato-fibrosis have used cirrhosis models induced with different chemicals and/or bile duct ligation, or with genetically engineered (Lee et al, 2005; Guimond et al, 2007; Yan et al, 2007; Lessa et al, 2010; Hayashi and Sakai, 2011; Chen et al, 2013). …”

Section: Discussionmentioning

confidence: 99%

Novel Approach for Evaluation of Bacteroides fragilis Protective Role against Bartonella henselae Liver Damage in Immunocompromised Murine Model

et al. 2016

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Bartonella henselae is a gram-negative facultative intracellular bacterium and is the causative agent of cat-scratch disease. Our previous data have established that Bacteroides fragilis colonization is able to prevent B. henselae damages through the polysaccharide A (PSA) in an experimental murine model. In order to determine whether the PSA is essential for the protection against pathogenic effects of B. henselae in immunocompromised hosts, SCID mice were co-infected with B. fragilis wild type or its mutant B. fragilis ΔPSA and the effects of infection on murine tissues have been observed by High-Frequency Ultrasound (HFUS), histopathological examination, and Transmission Electron Microscopy (TEM). For the first time, echostructure, hepatic lobes length, vascular alterations, and indirect signs of hepatic dysfunctions, routinely used as signs of disease in humans, have been analyzed in an immunocompromised murine model. Our findings showed echostructural alterations in all infected mice compared with the Phosphate Buffer Solution (PBS) control group; further, those infected with B. henselae and co-infected with B. henselae/B. fragilis ΔPSA presented the major echostructural alterations. Half of the mice infected with B. henselae and all those co-infected with B. henselae/B. fragilis ΔPSA have showed an altered hepatic echogenicity compared with the renal cortex. The echogenicity score of co-infected mice with B. henselae/B. fragilis ΔPSA differed significantly compared with the PBS control group (p < 0.05). Moreover the inflammation score of the histopathological evaluation was fairly concordant with ultrasound findings. Ultrastructural analysis performed by TEM revealed no significant alterations in liver samples of SCID mice infected with B. fragilis wild type while those infected with B. fragilis ΔPSA showed the presence of collagen around the main vessels compared with the PBS control group. The liver samples of mice infected with B. henselae showed macro-areas rich in collagen, stellate cells, and histiocytic cells. Interestingly, our data demonstrated that immunocompromised SCID mice infected with B. henselae and co-infected with B. henselae/B. fragilis ΔPSA showed the most severe morpho-structural liver damage. In addition, these results suggests that the HFUS together with histopathological evaluation could be considered good imaging approach to evaluate hepatic alterations.

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“…Yi Guan Jian (YGJ) is a traditional Chinese formula used to treat diseases induced by reduced liver health, and previous studies have demonstrated that YGJ may reverse liver cirrhosis ( 7 , 8 ). However, the effect of YGJ on BM-MSCs remains to be elucidated.…”

Section: Introductionmentioning

confidence: 99%

Yi Guan Jian decoction may enhance hepatic differentiation of bone marrow-derived mesenchymal stem cells via SDF-1 in vitro

Pang

Zhu

et al. 2017

Molecular Medicine Reports

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A previous study reported that Yi Guan Jian (YGJ) may increase the proliferation and differentiation of hepatic oval cells in a rat liver cirrhosis model. The aim of the present study was to investigate the effect and mechanism of action of YGJ on inducing hepatic differentiation in bone marrow-derived mesenchymal stem cells (BM-MSCs) via stromal-cell derived factor-1 (SDF-1). Murine BM-MSCs were isolated with whole bone marrow adherence, then identified by immunocytochemical staining and flow cytometry. Passage 2 cells were divided into 8 groups and their differentiation was induced by cell factors added to the medium, including hepatocyte growth factor (HGF), SDF-1 and YGJ. Each of the cell factors was used alone and any two or three of them were combined to establish different cell microenvironments in the different treatment groups. Albumin (ALB) was selected as a hepatocellular marker and cytokeratin-18 (CK-18) as a cholangiocellular marker. The protein and mRNA expression levels of ALB and CK-18 were used to determine the differentiation of BM-MSCs using immunocytochemical staining, western blotting and reverse transcription-quantitative polymerase chain reaction on days 7, 14, 21 and 28 during induction. The relative expression levels of ALB and CK-18 resulted in time-dependent increases in the groups supplemented only with HGF, SDF-1 or YGJ. Combination treatment of any two HGF, SDF-1 and YGJ led to a higher expression of ALB and CK-18 compared with only one cell factor treatment. Additionally, when all three were used in a combined treatment the expression levels of ALB and CK-18 occurred at an earlier time and was higher overall. Therefore, the present study suggested that YGJ had an effect on inducing hepatic differentiation in BM-MSCs via SDF-1 and may act in a synergistic manner with HGF and SDF-1.

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High-Frequency Ultrasound Imaging to Evaluate Liver Fibrosis Progression in Rats and Yi Guan Jian Herbal Therapeutic Effects

Cited by 17 publications

References 36 publications

Periodontal disease may induce liver fibrosis in an experimental study on Wistar rats

Periodontal disease may induce liver fibrosis in an experimental study on Wistar rats

Novel Approach for Evaluation of Bacteroides fragilis Protective Role against Bartonella henselae Liver Damage in Immunocompromised Murine Model

Yi Guan Jian decoction may enhance hepatic differentiation of bone marrow-derived mesenchymal stem cells via SDF-1 in vitro

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