High-Frequency Viscoelastic Shear Properties of Vocal Fold Tissues: Implications for Vocal Fold Tissue Engineering

Teller, Seth; Farran, Alexandra J. E.; Xiao, Longxi; Jiao, Tifeng; Duncan, Randall L.; Clifton, R. J.; Jia, Xinqiao

doi:10.1089/ten.tea.2012.0023

Cited by 24 publications

(34 citation statements)

References 48 publications

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“…The modular, recombinantly synthesized RLPs can be rapidly cross-linked via a Mannich-type condensation reaction to yield hydrogels with mechanical properties—storage shear moduli (500 Pa to 10 kPa), Young's moduli (15–35 kPa), resilience values (>90%), and storage shear moduli at high frequency (1000–2000 Pa)—that are consistent with the reported mechanical properties of native vocal fold tissues (Jiao et al, 2009; Teller et al, 2012). These RLPs also exhibit enzymatic-triggered degradation, facilitate the 2D adhesion and spreading of various cell types, and support the 3D encapsulation and survival of hMSCs in vitro , offering opportunities for fabricating either implantable or injectable scaffolds for vocal fold tissue therapies (Charati et al, 2009; Li et al, 2011, 2013; McGann et al, 2013).…”

Section: Introductionsupporting

confidence: 80%

Transient dynamic mechanical properties of resilin-based elastomeric hydrogels

Kiick

2014

Front. Chem.

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The outstanding high-frequency properties of emerging resilin-like polypeptides (RLPs) have motivated their development for vocal fold tissue regeneration and other applications. Recombinant RLP hydrogels show efficient gelation, tunable mechanical properties, and display excellent extensibility, but little has been reported about their transient mechanical properties. In this manuscript, we describe the transient mechanical behavior of new RLP hydrogels investigated via both sinusoidal oscillatory shear deformation and uniaxial tensile testing. Oscillatory stress relaxation and creep experiments confirm that RLP-based hydrogels display significantly reduced stress relaxation and improved strain recovery compared to PEG-based control hydrogels. Uniaxial tensile testing confirms the negligible hysteresis, reversible elasticity and superior resilience (up to 98%) of hydrated RLP hydrogels, with Young's modulus values that compare favorably with those previously reported for resilin and that mimic the tensile properties of the vocal fold ligament at low strain (<15%). These studies expand our understanding of the properties of these RLP materials under a variety of conditions, and confirm the unique applicability, for mechanically demanding tissue engineering applications, of a range of RLP hydrogels.

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Section: Introductionsupporting

confidence: 80%

Transient dynamic mechanical properties of resilin-based elastomeric hydrogels

Kiick

2014

Front. Chem.

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“…Their mechanical properties and degradation kinetics can be readily tuned by varying the particle size, functional group density, and intra- and interparticle crosslinking. Using a custom-designed torsional wave apparatus [187], we demonstrated that the viscoelastic properties of HA DXNs can be matched to that of the vocal fold tissue samples [188] at frequencies close to human phonation. Thus, these materials are attractive injectables for the elimination of vocal fold scarring [178, 189].…”

Section: Ha In Biomedical Applicationsmentioning

confidence: 99%

Hyaluronan: A simple polysaccharide with diverse biological functions

Dicker¹,

Gurski²,

et al. 2014

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Hyaluronan (HA) is a linear polysaccharide with disaccharide repeats of D-glucuronic acid and N-acetyl-D-glucosamine. It is evolutionary conserved and abundantly expressed in the extracellular matrix (ECM), on the cell surface and even inside cells. Being a simple polysaccharide, HA exhibits an astonishing array of biological functions. HA interacts with various proteins or proteoglycans to organize the ECM and to maintain tissue homeostasis. The unique physical and mechanical properties of HA contribute to the maintenance of tissue hydration, the mediation of solute diffusion through the extracellular space and the lubrication of certain tissues. The diverse biological functions of HA are manifested through its complex interactions with matrix components and resident cells. Binding of HA with cell surface receptors activates various signaling pathways that regulate cell function, tissue development, inflammation, wound healing and tumor progression and metastasis. Taking advantage of the inherent biocompatibility and biodegradability of HA, as well as its susceptibility to chemical modification, researchers have developed various HA-based biomaterials and tissue constructs with promising and broad clinical potential. In this article, we illustrate the properties of HA from a matrix biology perspective by first introducing principles underlying the biosynthesis and biodegradation of HA, as well as the interactions of HA with various proteins and proteoglycans. We next highlight the roles of HA in physiological and pathological states, including morphogenesis, wound healing and tumor metastasis. A deeper understanding of the mechanisms underlying the roles of HA in various physiological processes can provide new insights and tools for the engineering of complex tissues and tissue models.

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“…[63,186,187] The resilin-like polypeptides (RLPs) are easily crosslinked to yield tunable elastic shear and Young’s moduli of values comparable to those measured for vocal fold tissues (500–5000Pa at low frequency (1–10Hz), 200–2000 at higher frequency (30–150Hz) and 10–50kPa at low strain (<15%), respectively). [18,188–190] Hydrated, crosslinked films of RLPs also show excellent extensibility (up to 300%), efficient recovery, negligible stress relaxation and hysteresis, as well as high resilience (97%) characterized via standard stress-strain cyclic tensile testing, suggesting their flexibility and utility to efficiently transmit mechanical forces. Encouragingly, the RLP-based hydrogels demonstrated positive cytocompatibility and supported the adhesion and proliferation of mouse NIH3T3 fibroblasts.…”

Section: Biomaterials In Development/researchmentioning

confidence: 99%

“…[17,198] The mechanical properties of excised vocal fold tissue have been measured under tension[199] or shear, at low and high frequencies. [18,88,190,200] Non-invasive methods have also been developed to determine the shear modulus of human vocal fold tissue in vivo by analyzing the mucosal wave propagation speed during phonation with the aid of high speed digital imaging and magnetic resonance imaging. [83]…”

Section: Bioreactor Developmentsmentioning

confidence: 99%

Tissue engineering-based therapeutic strategies for vocal fold repair and regeneration

Stiadle

Lau

et al. 2016

Biomaterials

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Vocal folds are soft laryngeal connective tissues with distinct layered structures and complex multicomponent matrix compositions that endow phonatory and respiratory functions. This delicate tissue is easily damaged by various environmental factors and pathological conditions, altering vocal biomechanics and causing debilitating vocal disorders that detrimentally affect the daily lives of suffering individuals. Modern techniques and advanced knowledge of regenerative medicine have led to a deeper understanding of the microstructure, microphysiology, and micropathophysiology of vocal fold tissues. State-of-the-art materials ranging from extracecullar-matrix (ECM)-derived biomaterials to synthetic polymer scaffolds have been proposed for the prevention and treatment of voice disorders including vocal fold scarring and fibrosis. This review intends to provide a thorough overview of current achievements in the field of vocal fold tissue engineering, including the fabrication of injectable biomaterials to mimic in vitro cell microenvironments, novel designs of bioreactors that capture in vivo tissue biomechanics, and establishment of various animal models to characterize the in vivo biocompatibility of these materials. The combination of polymeric scaffolds, cell transplantation, biomechanical stimulation, and delivery of antifibrotic growth factors will lead to successful restoration of functional vocal folds and improved vocal recovery in animal models, facilitating the application of these materials and related methodologies in clinical practice.

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High-Frequency Viscoelastic Shear Properties of Vocal Fold Tissues: Implications for Vocal Fold Tissue Engineering

Cited by 24 publications

References 48 publications

Transient dynamic mechanical properties of resilin-based elastomeric hydrogels

Transient dynamic mechanical properties of resilin-based elastomeric hydrogels

Hyaluronan: A simple polysaccharide with diverse biological functions

Tissue engineering-based therapeutic strategies for vocal fold repair and regeneration

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