High FUT3 expression is a marker of lower overall survival of breast cancer patients

O-linked α2,3 sialylation defines stem cell populations in breast cancer

“…1C ). This finding intrigued us because FUT3 is an α1,3 fucosyltransferase that is associated with the expression of N-linked sLeX, which has been implicated in aggressive breast cancer ( 24 , 25 ). However, we found that FUT3 expression is significantly higher in epithelial basal A breast cancer cell lines compared with mesenchymal basal B cell lines by analyzing the Cancer Cell Line Encyclopedia (CCLE) database ( Fig.…”

“…An unexpected finding in this study is that FUT3, a key enzyme in the genesis of sLeX, antagonizes CSC function. Both FUT3 and sLeX have been implicated in breast cancer progression and correlate with lower overall survival of patients ( 24 , 25 ). sLeX is the main ligand for E-selectin, which is expressed on endothelial cells, and the binding of sLeX on tumors to E-selectin on endothelial cells contributes to extravasation from the vasculature during metastasis ( 24 ).…”

“…Both FUT3 and sLeX have been implicated in breast cancer progression and correlate with lower overall survival of patients ( 24 , 25 ). sLeX is the main ligand for E-selectin, which is expressed on endothelial cells, and the binding of sLeX on tumors to E-selectin on endothelial cells contributes to extravasation from the vasculature during metastasis ( 24 ). Our data reveal that sLeX antagonizes CSC function, including the function of CD44, and they highlight distinct differences in glycosylation between cells with stem cell properties and other populations of tumor cells.…”

(Sialyl)Lewis Antigen Expression on Glycosphingolipids, N-, and O-Glycans in Colorectal Cancer Cell Lines is Linked to a Colon-Like Differentiation Program

“…3 A ) which suggests associations with the carcinogenesis of CRC ( 74 ). Besides, upregulation of FUT3 is a marker of lower overall survival of breast cancer ( 75 ), and knockdown of FUT3 inhibits the proliferation, migration, tumorigenesis, and TGF-β induced EMT in pancreatic cancer ( 76 ). FUT4 contributes to the biosynthesis of (s)Le X ( 37 , 38 , 39 ) which was observed to be positively correlated with (s)Le A/X on GSL glycans but, interestingly, not with N - and O -glycans ( Fig.…”

Exploring the diverse biological significance and roles of fucosylated oligosaccharides

“…Therefore, the expression of these antigens, which normally interact with pathogens, in circulating cancer cells facilitates the metastatic process ( Miyoshi et al, 2012 ). The FUT3 gene is also named as the Lewis gene, and high expression of the tetrasaccharide sialic Lewis X, a product of this gene, has been reported in various malignant tumors such as gastric, pancreatic, breast, and ovarian cancer ( Mare et al, 2013 ; Bassagañas et al, 2015 ; do Nascimento et al, 2020 ; Dallolio et al, 2021 ). A study has shown that migration, invasion, and proliferation can be inhibited in gastric carcinoma by silencing the FUT3 gene ( Cai et al, 2016 ).…”