High generation dendrimer decorated poly-Ɛ-caprolactone/polyacrylic acid electrospun nanofibers for the design of a bioelectrochemical sensing surface

Oner, Aylis; Tufek, Ensara; Yezer, Irem; Birol, Asli; Demir, Meltem; Er, Simge; Demirkol, Dilek Odacı

doi:10.1016/j.reactfunctpolym.2021.104853

Cited by 23 publications

(10 citation statements)

References 53 publications

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“…SD and cv% values were determined as ± 0.22 and 2.31%, respectively. A cv% value below 5% increases the precision of the method [ 57 , 68 ]. It can be said that the PS/rGO-MNP-PDA/Anti-CRP immunosensor provides us high precision.…”

Section: Resultsmentioning

confidence: 99%

Graphene Oxide-Magnetic Nanoparticles Loaded Polystyrene-Polydopamine Electrospun Nanofibers Based Nanocomposites for Immunosensing Application of C-Reactive Protein

Ketmen

Gelen

et al. 2022

Biosensors

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The large surface area/volume ratio and controllable surface conformation of electrospun nanofibers (ENFs) make them highly attractive in applications where a large surface area is desired, such as sensors and affinity membranes. In this study, nanocomposite-based ENFs were produced and immobilization of Anti-CRP was carried out for the non-invasive detection of C-reactive protein (CRP). Initially, the synthesis of graphene oxide (GO) was carried out and it was modified with magnetic nanoparticles (MNP, Fe3O4) and polydopamine (PDA). Catechol-containing and quinone-containing functional groups were created on the nanocomposite surface for the immobilization of Anti-CRP. Polystyrene (PS) solution was mixed with rGO-MNP-PDA nanocomposite and PS/rGO-MNP-PDA ENFs were produced with bead-free, smooth, and uniform. The surface of the screen-printed carbon electrode (SPCE) was covered with PS/rGO-MNP-PDA ENFs by using the electrospinning technique under the determined optimum conditions. Next, Anti-CRP immobilization was carried out and the biofunctional surface was created on the PS/rGO-MNP-PDA ENFs coated SPCE. Moreover, PS/rGO-PDA/Anti-CRP and PS/MNP-PDA/Anti-CRP immunosensors were also prepared and the effect of each component in the nanocomposite-based electrospun nanofiber (MNP, rGO) on the sensor response was investigated. The analytic performance of the developed PS/rGO-MNP-PDA/Anti-CRP, PS/rGO-PDA/Anti-CRP, and PS/MNP-PDA/Anti-CRP immunosensors were examined by performing electrochemical measurements in the presence of CRP. The linear detection range of PS/rGO-MNP-PDA/Anti-CRP immunosensor was found to be from 0.5 to 60 ng/mL and the limit of detection (LOD) was calculated as 0.33 ng/mL for CRP. The PS/rGO-MNP-PDA/Anti-CRP immunosensor also exhibited good repeatability with a low coefficient of variation.

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Section: Resultsmentioning

confidence: 99%

Graphene Oxide-Magnetic Nanoparticles Loaded Polystyrene-Polydopamine Electrospun Nanofibers Based Nanocomposites for Immunosensing Application of C-Reactive Protein

Ketmen

Gelen

et al. 2022

Biosensors

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“…The cv % and SD values were calculated as 4.44% and ±0.13, respectively. Since the low value of cv % increases the precision of the method, a cv % value of less than 5% proves that the developed immunosensor offers us high precision. , Stability studies were also performed to evaluate how to change the PS/MWCNT-PAMAM/Anti-CD36 immunosensor response when the immunosensor was stored at 4 °C for 5 days. The current response of the PS/MWCNT-PAMAM/Anti-CD36 immunosensors only had a change of 0.54% decrease according to the initial response after 5 days (Figure b).…”

Section: Resultsmentioning

confidence: 99%

Carbon Nanotube-Incorporated Nanofibers for Immunosensor Preparation against CD36

Demirkol

2023

ACS Omega

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The increased serum concentration of CD36 is significantly associated with atherosclerosis, insulin resistance, and diabetes mellitus. Currently, there is no sensor system used for the detection of CD36 in the clinical field. Therefore, there is a need to develop a sensor system for the detection of CD36. The large surface area/volume ratio and controllable surface conformation of electrospun nanofibers (ENs) make them highly attractive for immunosensor applications. In the present study, PS/MWCNT-PAMAM ENs were produced and used as an immobilization matrix of Anti-CD36. Thus, the electrochemical behavior of the developed nanocomposite-based ENs and their usage potential were investigated for immunosensor applications. First, an oxidized multiwall carbon nanotube (MWCNT-OH) was synthesized and modified with a polyamidoamine generation 3 (PAMAM G3) dendrimer. The synthesized MWCNT-PAMAM nanocomposite was mixed with polystyrene (PS) solutions at different ratios to produce bead-free, smooth, and uniform PS/MWCNT-PAMAM ENs. PS/MWCNT-PAMAM ENs were accumulated on a screen-printed carbon electrode (SPCE) using the electrospinning technique. A biofunctional surface on the PS/MWCNT-PAMAM EN-coated SPCE was created using carbodiimide chemistry by covalent immobilization of Anti-CD36. The analytic performance characteristics of the developed PS/MWCNT-PAMAM/Anti-CD36 immunosensor were determined by performing electrochemical measurements in the presence of the CD36 protein. The linear detection range was found to be from 5 to 40 ng/mL, and the limit of detection was calculated as 3.94 ng/mL for CD36. The developed PS/MWCNT-PAMAM/Anti-CD36 immunosensor also displayed high tolerance to interference substances, good repeatability, and high recovery percent (recovery%) for artificial blood serum analysis.

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“…A significant application of the stimuli-responsive materials is development of chemosensors, and polymer nanoparticles are among the most significant carriers for this purpose. ,− Application of chemosensors based on polymer nanoparticles encountered some issues because of insolubility of polymers in aqueous media. Therefore, hydrophilic dendritic structures have gained much attention in the preparation of chemosensors applicable in aqueous media. ,,,− A new approach has recently been used for the development of chemosensors by doping photoactive or photoluminescent molecules into the dendrimer structure. In this strategy, the light-responsive molecules, such as photoluminescent and photochromic organic compounds, are incorporated into the structure of dendrimers by chemical or physical modification strategies.…”

Section: Resultsmentioning

confidence: 99%

Stimuli-Responsive Dendritic Macromolecules for Optical Detection of Metal Ions and Acidic Vapors by the Photoinduced Electron Transfer Mechanism: Paper-Based Indicator for Food Spoilage Sensing

Razavi

Roghani‐Mamaqani

Salami‐Kalajahi

2022

ACS Appl. Mater. Interfaces

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Visual detection of analytes has been a significant challenge in the design and development of optical chemosensors. Sensing of analytes in aqueous solution by organic molecules has encountered some issues, such as poor water solubility and quenching of optical properties. In this study, a new category of smart dendritic macromolecules was designed and synthesized by functionalization of the poly(amidoamine) (PAMAM) dendrimer with spiropyran molecules to afford a photoluminescent dendritic structure (SP-PAMAM). Smart optical sensors were prepared by physical incorporation of four different oxazolidine derivatives containing hydroxyl and nitro substituted groups into the SP-PAMAM structure. Investigation of optical properties demonstrated photoinduced electron transfer (PET) between the spiropyran end group of SP-PAMAM and oxazolidine derivatives (in a concentration of about 0.0002 M), which can result in quenching of fluorescence emission of spiropyran photoswitch in the form of merocyanine (MC). Treatment of the oxazolidinedoped SP-PAMAM samples with metal ions resulted in changes in the PET mechanism (switching on or off), as observed in the case of Fe 3+ , Pb 2+ , Cu 2+ , Zn 2+ , Cd 2+ , Co 2+ , and Ni 2+ by different oxazolidine derivatives through various mechanisms (increase or decrease of fluorescence emission). These smart photoluminescent dendritic macromolecules have potential applications for photodetection of metal ions in aqueous media as optical chemosensors. In addition, the smart macromolecules displayed disconnection of PET between MC and oxazolidine and also showed red fluorescence emission under acidic conditions (pH 1−5). It is due to the protonation of the MC to MCH form and demonstrates a remarkable red shift in fluorescence spectra. The pH-responsivity of smart macromolecules was used for designing a paper-based pH indicator for visual detection of spoilage in the food industry, especially in the case of milk. The prepared papers applied on cap of the milk bottles did not show any fluorescence emission in the case of fresh milk; however, a red fluorescence emission was observed after milk spoilage as a result of adsorption of acidic volatile components generated by bacterial degradation and oxidation process on the paper surface. The reported smart papers can serve as optical portable pH indicators for timely detection of spoilage in food materials, which are usable in food packaging as smart indicator tags.

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High generation dendrimer decorated poly-Ɛ-caprolactone/polyacrylic acid electrospun nanofibers for the design of a bioelectrochemical sensing surface

Cited by 23 publications

References 53 publications

Graphene Oxide-Magnetic Nanoparticles Loaded Polystyrene-Polydopamine Electrospun Nanofibers Based Nanocomposites for Immunosensing Application of C-Reactive Protein

Graphene Oxide-Magnetic Nanoparticles Loaded Polystyrene-Polydopamine Electrospun Nanofibers Based Nanocomposites for Immunosensing Application of C-Reactive Protein

Carbon Nanotube-Incorporated Nanofibers for Immunosensor Preparation against CD36

Stimuli-Responsive Dendritic Macromolecules for Optical Detection of Metal Ions and Acidic Vapors by the Photoinduced Electron Transfer Mechanism: Paper-Based Indicator for Food Spoilage Sensing

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