High genetic diversity in Aegilops tauschii Coss. accessions from North Iran as revealed by IRAP and REMAP markers

Abstract

Background Aegilops tauschii Coss. as a donor of wheat D genome has an important role in wheat breeding programs. Genetic and phylogeographic diversity of 79 Ae. tauschii accessions collected from north and northwest of Iran were analyzed based on retroelement insertional polymorphisms using inter-retrotransposon amplified polymorphism (IRAP) and retrotransposon-microsatellite amplified polymorphism (REMAP) markers. Results In total, 306 and 151 polymorphic bands were amplified in IRAP and REMAP analyses, respectively. As a result, a high level of polymorphism was observed among the studied accessions as revealed by an average of 25.5 bands per primer/primer combination and mean PIC value of 0.47 in IRAP and an average of 25.16 bands per primer combination and mean PIC value of 0.47 in REMAP. Genetic relationships of the accessions were analyzed using distance- and model-based cluster analyses. Conclusion The result showed that genetic distance did not seem to be related to geographic distribution, and the accessions could be divided into three groups, which was further supported by principal coordinate analysis. These results on genetic diversity and population structure of Ae. tauschii in Iran should provide important knowledge on genetic resources and their applications in wheat breeding programs.

Analysis of physio-biochemical responses and expressional profiling of DREB transcription factors for drought tolerance in Aegilops tauschii Coss

“…tauschii, which partially or completely suppressed the qP activity. Moreover, drought and other abiotic stresses also affect large-scale chlorophyll uorescence in Beta vulgaris, Coronnilla varia, P. mongolica and L. ruthenicum [50,51]. Drought stress can destroy plant cell membrane systems [52], and many studies have shown that CAT, POD, MDA, and free proline accumulate in plants under drought stress to maintain the dynamic balance of the antioxidant system [53,54].…”