High genetic heterogeneity of Mycobacterium intracellulare isolated from respiratory specimens

Lari, Nicoletta; Rindi, Laura

doi:10.1186/s12866-021-02426-5

Cited by 2 publications

(3 citation statements)

References 20 publications

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“…Our results show that the genetic characteristics of clinical isolates of M. intracellulare are not related to geographical location, which is consistent with the previous reports with VNTR analyses ( 29 , 30 ). In contrast to M. intracellulare , the genetic characteristics and molecular epidemiology of clinical strains of the MP-MIP group are poorly understood.…”

Section: Discussionsupporting

confidence: 93%

“…With genetic sequencing increasingly affordable, the MP-MIP group strains can be detected more frequently in the future. Though variable numbers of tandem repeats (VNTR) analysis has been a highly discriminatory tool in molecular epidemiology analysis, it is unable to classify the M. intracellulare and related strains such as MP and MIP ( 29 ). A previous study found that there are 4% of M. intracellulare isolates that have been identified as MIP by sequence-based typing analyses ( 15 , 16 ).…”

Section: Discussionmentioning

confidence: 99%

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High diversity of clinical Mycobacterium intracellulare in China revealed by whole genome sequencing

Song

Li²,

Ma³

et al. 2022

Front. Public Health

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Mycobacterium intracellulare is the most common cause of nontuberculous mycobacterial lung disease, with a rapidly growing prevalence worldwide. In this study, we performed comparative genomic analysis and antimicrobial susceptibility characteristics analysis of 117 clinical M. intracellulare strains in China. Phylogenetic analysis showed that clinical M. intracellulare strains had high genetic diversity and were not related to the geographical area. Notably, most strains (76.07%, 89/117) belonged to Mycobacterium paraintracellulare (MP) and Mycobacterium indicus pranii (MIP) in the genome, and we named them MP-MIP strains. These MP-MIP strains may be regarded as a causative agent of chronic lung disease. Furthermore, our data demonstrated that clarithromycin, amikacin, and rifabutin showed strong antimicrobial activity against both M. intracellulare and MP-MIP strains in vitro. Our findings also showed that there was no clear correlation between the rrs, rrl, and DNA gyrase genes (gyrA and gyrB) and the aminoglycosides, macrolides, and moxifloxacin resistance, respectively. In conclusion, this study highlights the high diversity of M. intracellulare in the clinical setting and suggests paying great attention to the lung disease caused by MP-MIP.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

High diversity of clinical Mycobacterium intracellulare in China revealed by whole genome sequencing

Song

Li²,

Ma³

et al. 2022

Front. Public Health

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“…Among them, M. intracellulare has been reported to be responsible for the largest proportion of MAC-PD cases ( Prevots and Marras, 2015 ; Chang et al, 2020 ; Ratnatunga et al, 2020 ). Mycobacterium intracellulare can be found in any environment, including soil, water, and air, and molecular epidemiology has revealed considerable genetic variation in its strains ( Hruska and Kaevska, 2012 ; Parker et al, 2020 ; Lari and Rindi, 2022 ). MAC reinfection and redevelopment of MAC-PD can result from exposure to these environmental sources or regrowth of previously infected strains.…”

Section: Introductionmentioning

confidence: 99%

A novel repeat sequence-based PCR (rep-PCR) using specific repeat sequences of Mycobacterium intracellulare as a DNA fingerprinting

Shin

Kim

et al. 2023

Front. Microbiol.

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Repetitive sequence-based PCR (rep-PCR) is a potential epidemiological technique that can provide high-throughput genotype fingerprints of heterogeneous Mycobacterium strains rapidly. Previously published rep-PCR primers, which are based on nucleotide sequences of Gram-negative bacteria may have low specificity for mycobacteria. Moreover, it was difficult to ensure the continuity of the study after the commercial rep-PCR kit was discontinued. Here, we designed a novel rep-PCR for Mycobacterium intracellulare, a major cause of nontuberculous mycobacterial pulmonary disease with frequent recurrence. We screened the 7,645 repeat sequences for 200 fragments from the genome of M. intracellulare ATCC 13950 in silico, finally generating five primers with more than 90% identity for a total of 226 loci in the genome. The five primers could make different band patterns depending on the genome of three different M. intracellulare strains using an in silico test. The novel rep-PCR with the five primers was conducted using 34 bacterial samples of 7 species containing 25 M. intracellulare clinical isolates, compared with previous published rep-PCRs. This shows distinguished patterns depending on species and blotting assay for 6 species implied the sequence specificity of the five primers. The Designed rep-PCR had a 95–98% of similarity value in the reproducibility test and showed 7 groups of fingerprints in M. intracellulare strains. Designed rep-PCR had a correlation value of 0.814 with VNTR, reference epidemiological method. This study provides a promising genotype fingerprinting method for tracing the recurrence of heterogeneous M. intracellulare.

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High genetic heterogeneity of Mycobacterium intracellulare isolated from respiratory specimens

Cited by 2 publications

References 20 publications

High diversity of clinical Mycobacterium intracellulare in China revealed by whole genome sequencing

High diversity of clinical Mycobacterium intracellulare in China revealed by whole genome sequencing

A novel repeat sequence-based PCR (rep-PCR) using specific repeat sequences of Mycobacterium intracellulare as a DNA fingerprinting

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