2020
DOI: 10.1128/msphere.00704-19
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High Genomic Diversity and Heterogenous Origins of Pathogenic and Antibiotic-Resistant Escherichia coli in Household Settings Represent a Challenge to Reducing Transmission in Low-Income Settings

Abstract: Escherichia coli is present in multiple hosts and environmental compartments as a normal inhabitant, temporary or persistent colonizer, and as a pathogen. Transmission of E. coli between hosts and with the environment is considered to occur more often in areas with poor sanitation. We performed whole-genome comparative analyses on 60 E. coli isolates from soils and fecal sources (cattle, chickens, and humans) in households in rural Bangladesh. Isolates from household soils were in multiple branches of the reco… Show more

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Cited by 34 publications
(34 citation statements)
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References 67 publications
(77 reference statements)
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“…In households like those in our study area, with low levels of access to sanitation and hygiene facilities, E . coli and other faecal indicators and pathogens are widespread on surfaces and in food produce [ 77 ] and it is likely that strains circulate between humans, animals, and the domestic environment [ 8 , 78 ]. Large-scale randomised control trials investigating the impact of water, sanitation, and hygiene interventions on health outcomes for communities in Kenya, Bangladesh and elsewhere have demonstrated the importance of considering multiple faecal exposure pathways [ 79 ].…”
Section: Resultsmentioning
confidence: 99%
“…In households like those in our study area, with low levels of access to sanitation and hygiene facilities, E . coli and other faecal indicators and pathogens are widespread on surfaces and in food produce [ 77 ] and it is likely that strains circulate between humans, animals, and the domestic environment [ 8 , 78 ]. Large-scale randomised control trials investigating the impact of water, sanitation, and hygiene interventions on health outcomes for communities in Kenya, Bangladesh and elsewhere have demonstrated the importance of considering multiple faecal exposure pathways [ 79 ].…”
Section: Resultsmentioning
confidence: 99%
“…Determined plasmid incompatibility (Inc.) types were IncFIC(FII) and IncI1. Both IncF- as well as IncI-type plasmids have been previously associated with the spread of ESBL-producing E. coli ( Coque et al, 2008 ; Branger et al, 2018 ; Montealegre et al, 2020 ). Group 3 genomes showed only small plasmid analogies with the Mongolian representatives and did not carry any ESBL-genes.…”
Section: Resultsmentioning
confidence: 99%
“…2). The bacterial isolates included 60 isolates hosting known β-lactamase genes previously characterized by Patrice Nordmann's lab (University of Fribourg, Fribourg, Switzerland, personal communication) and 14 Escherichia coli isolates from Bangladesh 27,28 . The bacterial genomic DNA was extracted by boiling at 100°C for 1 h. Ligation and PCR proceeded as described, with a probe pair mix totalling 1 μM, and 2 probe pairs per cluster for 18 clusters.…”
Section: Methodsmentioning
confidence: 99%