High Glucose Enhances the Odonto/Osteogenic Differentiation of Stem Cells from Apical Papilla via NF-KappaB Signaling Pathway

Wang, Yanping; Wang, Yanqiu; Lu, Yadie; Yu, Jinhua

doi:10.1155/2019/5068258

Cited by 9 publications

(14 citation statements)

References 55 publications

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“…Wang et al . [ 14 ] support the study results of Chen et al . [ 12 ] that NF-κB is activated by high glucose, causing decreased stem cell proliferation, but enhancing differentiation of these stem cells.…”

Section: Discussionsupporting

confidence: 87%

“…DM affects both DESCs and the dental papilla stem cells during odontogenesis, thereby affecting enamel, dentin, pulp, and whole of periodontium. Their molecular pathways are brought into limelight by the four articles[ 2 12 13 14 ] taken for our review. Of the four studies, two[ 2 12 ] were by the same authors conducted on their animal model.…”

Section: Discussionmentioning

confidence: 99%

“…The fourth article considered in this review by Wang et al [ 14 ] is an in vitro study where the stem cells from the apical papilla (SCAPs) were isolated from the immature dental roots and cultured. MTT assay abbreviated as 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2,5-tetrazoliumbromide, alkaline phosphatase activity monitoring, alizarin red staining, RT-PCR, and Western blot analysis were performed.…”

Section: R Esultsmentioning

confidence: 99%

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Molecular insight into odontogenesis in hyperglycemic environment: A systematic review

et al. 2020

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A BSTRACT Diabetes mellitus is an endocrinal disorder affecting worldwide and the disease incidence is rising alarmingly high. The effects of diabetes on tooth development are explored by limited studies and their molecular insights are very rarely studied. This systematic review is aimed to provide the best scientific literature source on the molecular insights into odontogenesis in hyperglycemic environment caused by diabetes mellitus or by maternal diabetes on the offspring. The literature search was conducted on the databases, namely PubMed, PubMed Central, Cochrane, and Scopus. The original studies exploring the alterations in the molecular pathways of odontogenesis in diabetes mellitus were selected. Data were extracted, chosen, and evaluated by two independent researchers. At the end of thorough data search, four articles were eligible for the review. Three articles brought out the molecular pathways involved in the offspring of gestational diabetes through animal models. Fourth article was an in vitro study, which treated the stem cells in hyperglycemic environment and drafted the molecular pathway. The altered molecular pathways in dental epithelial stem cells (DESCs), dental papilla cells (DPCs), and stem cells from apical papilla were studied and empowered with statistical analysis. Thus with this systematic review, we conclude that apurinic/apyrimidinic endonuclease1 downregulation causing deoxyribonucleic acid hypermethylation and Oct4, Nanog gene silencing, activation of toll-like receptor-4/nuclear factor kappa B (TLR4/NF-κB) pathway are involved in suppressing cell proliferation and accelerated apoptosis in DESCs in high glucose environment. DPCs are suppressed from odonto differentiation by activation of TLR4 signaling and resulting inhibition of SMAD1/5/9 phosphorylation in diabetic condition. NF-κB pathway activation causes decreased cell proliferation and enhanced differentiation in apical papilla stem cells in hyperglycemia. Further studies targeting various stages of odontogenesis can reveal more molecular insight.

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Section: Discussionsupporting

confidence: 87%

Section: Discussionmentioning

confidence: 99%

Section: R Esultsmentioning

confidence: 99%

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Molecular insight into odontogenesis in hyperglycemic environment: A systematic review

et al. 2020

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“…However, continuous growth can be attributed to the new tooth‐forming cells that are derived from stem cells. It is possible that an upregulated NF‐κB pathway component might fully or partially affect these dental stem cells, which might further lead to enamel defects in the mouse incisor, as the NF‐κB pathway has been shown to be involved in the regulation of stem cells (Chen et al., 2019; Shen et al., 2019; Wang, Wang, Lu, & Yu, 2019). However, abnormal enamel in patients is not caused by the defects in stem cells.…”

Section: Discussionmentioning

confidence: 99%

Overactivation of the NF‐κB pathway impairs molar enamel formation

et al. 2020

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Objective Hypohidrotic ectodermal dysplasia (HED) is a hereditary disorder characterized by abnormal structures and functions of the ectoderm‐derived organs, including teeth. HED patients exhibit a variety of dental symptoms, such as hypodontia. Although disruption of the EDA/EDAR/EDARADD/NF‐κB pathway is known to be responsible for HED, it remains unclear whether this pathway is involved in the process of enamel formation. Experimental subjects and methods To address this question, we examined the mice overexpressing Ikkβ (an essential component required for the activation of NF‐κB pathway) under the keratin 5 promoter (K5‐Ikkβ). Results Upregulation of the NF‐κB pathway was confirmed in the ameloblasts of K5‐Ikkβ mice. Premature abrasion was observed in the molars of K5‐Ikkβ mice, which was accompanied by less mineralized enamel. However, no significant changes were observed in the enamel thickness and the pattern of enamel rods in K5‐Ikkβ mice. Klk4 expression was significantly upregulated in the ameloblasts of K5‐Ikkβ mice at the maturation stage, and the expression of its substrate, amelogenin, was remarkably reduced. This suggests that abnormal enamel observed in K5‐Ikkβ mice was likely due to the compromised degradation of enamel protein at the maturation stage. Conclusion Therefore, we could conclude that the overactivation of the NF‐κB pathway impairs the process of amelogenesis.

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“…In 2020, Deng et al[ 98 ] reported that platelet derived growth factor BB promoted SCAPs osteogenic differentiation and enhanced bone formation in calvarial defects combined with a thermosensitive hydrogel[ 98 ]. Both conditioned culture medium containing traditional Chinese herbal remedy, Yunnan Baiyao, and high glucose α-Minimal Essential Medium can promote the odonto/osteogenic differentiation of SCAPs through the nuclear factor κB signaling pathway[ 99 , 100 ]. Depletion of lysine-specific demethylase 2A enhanced the adipogenic and chondrogenic differentiation potentials of SCAPs[ 101 ].…”

Section: Diverse Differentiation Of Dmscsmentioning

confidence: 99%

Multidifferentiation potential of dental-derived stem cells

Yin¹,

Luo²,

Feng³

et al. 2021

WJSC

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Tooth-related diseases and tooth loss are widespread and are a major public health issue. The loss of teeth can affect chewing, speech, appearance and even psychology. Therefore, the science of tooth regeneration has emerged, and attention has focused on tooth regeneration based on the principles of tooth development and stem cells combined with tissue engineering technology. As undifferentiated stem cells in normal tooth tissues, dental mesenchymal stem cells (DMSCs), which are a desirable source of autologous stem cells, play a significant role in tooth regeneration. Researchers hope to reconstruct the complete tooth tissues with normal functions and vascularization by utilizing the odontogenic differentiation potential of DMSCs. Moreover, DMSCs also have the ability to differentiate towards cells of other tissue types due to their multipotency. This review focuses on the multipotential capacity of DMSCs to differentiate into various tissues, such as bone, cartilage, tendon, vessels, neural tissues, muscle-like tissues, hepatic-like tissues, eye tissues and glands and the influence of various regulatory factors, such as non-coding RNAs, signaling pathways, inflammation, aging and exosomes, on the odontogenic/osteogenic differentiation of DMSCs in tooth regeneration. The application of DMSCs in regenerative medicine and tissue engineering will be improved if the differentiation characteristics of DMSCs can be fully utilized, and the factors that regulate their differentiation can be well controlled.

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High Glucose Enhances the Odonto/Osteogenic Differentiation of Stem Cells from Apical Papilla via NF-KappaB Signaling Pathway

Cited by 9 publications

References 55 publications

Molecular insight into odontogenesis in hyperglycemic environment: A systematic review

Molecular insight into odontogenesis in hyperglycemic environment: A systematic review

Overactivation of the NF‐κB pathway impairs molar enamel formation

Multidifferentiation potential of dental-derived stem cells

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