2003
DOI: 10.1152/ajpcell.00192.2002
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High glucose-induced, endothelin-dependent fibronectin synthesis is mediated via NF-κB and AP-1

Abstract: Human endothelial cells cultured under high glucose (HG) conditions were shown before to upregulate several basement membrane proteins, including fibronectin (FN), thus mimicking effects of diabetes. Using human macrovascular (HUVEC) and microvascular (HMEC) endothelial cell lines, we evaluated in the present study some of the key molecular signaling events involved in HG-induced FN overexpression. This expression was shown to be dependent on endogenous endothelin (ET) receptor-mediated signaling. We also exam… Show more

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Cited by 123 publications
(170 citation statements)
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“…6 Subconfluent cells were incubated with either 5 mM D-glucose (control) or 25 mM D-glucose (high glucose; HG). Cells in 5 mM glucose were also treated with 5 nM ET-1 (Peninsula Laboratories, Belmont, CA, USA).…”
Section: Endothelial Cellsmentioning
confidence: 99%
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“…6 Subconfluent cells were incubated with either 5 mM D-glucose (control) or 25 mM D-glucose (high glucose; HG). Cells in 5 mM glucose were also treated with 5 nM ET-1 (Peninsula Laboratories, Belmont, CA, USA).…”
Section: Endothelial Cellsmentioning
confidence: 99%
“…6 Briefly, NF-kB and AP-1 consensus oligonucleotide DNA probes (Promega, WI, USA) ( Table 2) were prepared by end labeling with [g-32 P] ATP (Amersham, Quebec, Canada) using T4 polynucleotide kinase. The probes were purified by ethanol precipitation and resuspended in 10 mM Tris and 1 mM EDTA (pH 7.6).…”
Section: Electrophoretic Mobility Shift Assaymentioning
confidence: 99%
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