High glucose levels inhibit focal adhesion kinase-mediated wound healing of rat peritoneal mesothelial cells

Tamura, Masahito; Osajima, Akihiko; Nakayamada, Shingo; Anai, Hirofumi; Kabashima, Narutoshi; Kanegae, Kaori; Ota, Takayuki; Tanaka, Yoshiya; Nakashima, Yasuhide

doi:10.1046/j.1523-1755.2003.00772.x

Cited by 47 publications

(48 citation statements)

References 36 publications

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“…In line with our observations, previous studies showed that high concentrations of glucose and GDPs suppress MC wound healing. High glucose inhibits integrin-mediated cell migration along the basal membrane (36), and GDPs decrease the velocity of MC wound closure and impair MC viability in vitro (37). Furthermore, compared with MCs taken from glucose effluents, those taken from icodextrin effluents show greater proliferation ex vivo (38).…”

Section: Discussionmentioning

confidence: 99%

A Peritoneal Dialysis Regimen Low in Glucose and Glucose Degradation Products Results in Increased Cancer Antigen 125 and Peritoneal Activation

et al. 2011

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♦ Background: Glucose and glucose degradation products (GDPs) in peritoneal dialysis fluids (PDFs) are both thought to mediate progressive peritoneal worsening. ♦ Methods: In a multicenter, prospective, randomized crossover study, incident continuous ambulatory peritoneal dialysis patients were treated either with conventional lactate-buffered PDF (sPD regimen) or with a regimen low in glucose and GDPs: Nutrineal×1, Extraneal×1, and Physioneal×2 (NEPP regimen; all solutions: Baxter Healthcare, Utrecht, Netherlands). After 6 months, patients were switched to the alternative regimen for another 6 months. After 6 weeks of run-in, before the switch, and at the end of the study, 4-hour peritoneal equilibration tests were performed, and overnight effluents were analyzed for cells and biomarkers. Differences between the regimens were assessed by multivariate analysis corrected for time and regimen sequence.

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Section: Discussionmentioning

confidence: 99%

A Peritoneal Dialysis Regimen Low in Glucose and Glucose Degradation Products Results in Increased Cancer Antigen 125 and Peritoneal Activation

et al. 2011

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“…They distribute widely on the surface of omentum and peritoneum and are easy to harvest with a small umbilical incision. They are also easy to be cultured in vitro and the commonly used DMEM medium could satisfy their nutritional requirement [10,11]. Mesothelium-lined grafts including peritoneum [12,13] and tunica vaginalis [14,15] have already been attempted in urethral reconstruction with good results.…”

Section: Discussionmentioning

confidence: 99%

Tubularized Urethral Replacement Using Tissue-Engineered Peritoneum-Like Tissue in a Rabbit Model

Xia²,

Zhang³

et al. 2012

Urol Int

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Introduction: This study aims to investigate whether mesothelial cells could function as seed cells to construct tissue-engineered peritoneum-like tissue for urethral reconstruction in a rabbit model. Materials and Methods: Bladder acellular matrices were prepared and trimmed to 1.5 × 1 cm. Nine male rabbits underwent omentum biopsy and autologous mesothelial cells were isolated. After in vitro expansion, the cells were seeded onto the matrices and incubated for 7 days. In 18 rabbits, a pendulous urethral segment 1.5 cm long was totally excised and urethroplasty was performed with tubularized matrices seeded with cells in 9 animals and without cells in 9 as controls. Urethrography was performed at 1, 2 and 6 months postoperatively. Meanwhile, the neourethras were harvested and analyzed grossly and histologically. Results: Histological analysis of the cell-seeded grafts revealed a loose collagen matrix covered with a single layer of mesothelim. Serial urethrography indicated a wide urethral caliber without stricture formation in animals implanted with cell-seeded matrices, while all animals of the control group developed stricture. Histological analysis of the implanted cell-seeded matrices demonstrated a normal urethral architecture by 1 month, composed of multilayers of urothelium surrounded by smooth muscle bundles, which became increasingly organized with time. By 6 months, the neourethra could be hardly distinguished from native urethra both grossly and histologically. Conclusions: Autologous mesothelial cells could be successfully used as seed cells for tubularized urethral reconstruction in male rabbits.

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“…FAK plays a critical role in cell survival and is phosphorylated when cells are treated by mannitol [14,21,42].…”

Section: Discussionmentioning

confidence: 99%

“…Among the cytoskeletal-associated proteins, FAK has been proposed to be a critical factor in modulating cytoskeletal changes [7,43]. Due to the fact that FAK activation is also known to be linked with PKC activity [8,15,17,42], FAK is likely to play a fundamental role in mesangial cell contraction.…”

Section: Introductionmentioning

confidence: 99%

Attenuation of Mouse Mesangial Cell Contractility by High Glucose and Mannitol: Involvement of Protein Kinase C and Focal Adhesion Kinase

et al. 2004

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Hyperglycemia and mannitol activate protein kinase C (PKC) and induce mesangial cell hypocontractility that subsequently may modulate renal function. Since focal adhesion kinase (FAK) activation is known to be linked with PKC activity, FAK may also be involved in mesangial cell contraction. To facilitate our understanding of the PKC- and FAK-modulating mechanism, we developed an in vitro model of mouse mesangial cell hypocontractility induced by hyperglycemia or mannitol. Mouse mesangial cells (CRL-1927) were exposed to: normal D-glucose (group N), high D-glucose (group H), and control groups at the same osmolality as H plus L-glucose (group L) and mannitol (group M). Changes in the planar surface area of cells in response to 1 µM phorbol 12-myristate 13-acetate (PMA) were determined. Western blot analyses for PKC, phosphorylated (p)-PKC, tyrosine phosphorylation, FAK, and p-FAK were done on each of these four groups. The effects of mannitol in various doses on cell contraction and activation of PKC and FAK were also assayed. The planar surface areas of groups H and M both showed an attenuated change in response to PMA stimulation. Before PMA stimulation, the baseline PKC expression of groups H and M showed a higher expression of p-PKCα and δ than that seen in group N (p < 0.05). Results of tyrosine phosphorylation and immunoprecipitation showed that FAK may be involved in this contraction process. The total amount of FAK showed no significant difference among the four experimental groups; however, p-FAK was found to have significantly increased in group M (p < 0.05). The use of PKC and tyrosine kinase inhibitors reduced PMA-induced mesangial cell contraction in all four groups. Activation of PKCα, δ, and FAK with the resultant inhibition of mesangial cell contraction by mannitol was found to be dose-dependent. These results may provide a correlation between increased expression of several PKC isoforms and, in particular, increased phosphorylation levels of PKCα and δ and hypocontractility induced by high glucose and mannitol treatment. Furthermore, the mannitol-induced hypocontractility involving PKC and FAK occurred in a dose-dependent manner.

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High glucose levels inhibit focal adhesion kinase-mediated wound healing of rat peritoneal mesothelial cells

Cited by 47 publications

References 36 publications

A Peritoneal Dialysis Regimen Low in Glucose and Glucose Degradation Products Results in Increased Cancer Antigen 125 and Peritoneal Activation

A Peritoneal Dialysis Regimen Low in Glucose and Glucose Degradation Products Results in Increased Cancer Antigen 125 and Peritoneal Activation

Tubularized Urethral Replacement Using Tissue-Engineered Peritoneum-Like Tissue in a Rabbit Model

Attenuation of Mouse Mesangial Cell Contractility by High Glucose and Mannitol: Involvement of Protein Kinase C and Focal Adhesion Kinase

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