High HIF-1α expression genotypes in oral lichen planus

Fraga, Carlos Alberto de Carvalho; Alves, Lucas Rodrigues; Marques-Silva, Luciano; Sousa, Adriana Alkmim de; Jorge, Amanda Lacerda; Jesus, Sabrina Ferreira de; Vilela, Daniel Nogueira; Pinheiro, Ugo Borges; Jones, Kimberly Marie; De-Paula, Alfredo Maurício Batista; Guimarães, André Luiz Sena

doi:10.1007/s00784-013-0920-8

Cited by 16 publications

(14 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The genetic polymorphism of the HIF1α gene affects the severity and outcome of infectious and inflammatory diseases. For example, it is established that the polymorphism of the gene HIF1α (1772T allele), which determines its high level of expression, is a risk factor for the development of abdominal aortic aneurysm and oral lichen planus 23,24. The other polymorphic HIF-1α rs12434439 GG genotype plays a protective role for rheumatoid arthritis development,25 but gene polymorphism HIF-1α rs11549467 is associated with the risk of COPD 26…”

Section: Introductionmentioning

confidence: 99%

<p>Dependence of the severity of the systemic inflammatory response on resistance to hypoxia in male Wistar rats</p>

Джалилова

Косырева

Diatroptov

et al. 2019

JIR

View full text Add to dashboard Cite

The aim of the study was to characterize the severity of the systemic inflammatory response induced by lipopolysaccharide (LPS) in animals with different resistance levels to hypoxia. Materials and methods: Two to three months old male Wistar rats (220-240 g) were divided according to hypoxia tolerance in a hypobaric chamber. After a month, they were injected intraperitoneally with Escherichia coli LPS at a dose of 1.5 mg/kg. After 3, 6 and 24 hours of LPS injection, we studied the levels of IL-1β, C-reactive protein (CRP) and TGF-β in the serum, the expression of Hif-1α and Nf-kb in the liver, morphological disorders in the lung and ex vivo production of IL-10 by splenic cells activated by ConA. Results: In the early periods after the injection of LPS, increase in Nf-kb expression in the liver was observed only in the rats susceptible to hypoxia. After 6 hours of LPS injection, the number of neutrophils in the interalveolar septa of the lungs of rats susceptible to hypoxia was higher than in tolerant rats. This points to the development of more pronounced LPS-induced inflammation in the rats susceptible to hypoxia and is accompanied by increased expression of Hif-1α in the liver after 6 hours of LPS administration, serum IL-1β level after 3 hours and CRP level after 24 hours. The production of the anti-inflammatory cytokine IL-10 by the spleen was significantly decreased after 6 hours of LPS injection only in the animals tolerant to hypoxia. After 24 hours of LPS injection, a significant decrease in serum TGF-β level occurred in the rats tolerant to hypoxia in comparison with the control group, which improved the survival rates of the animals. Conclusion: We have demonstrated the differences in the severity of the LPS-induced inflammatory response in male Wistar rats with different resistance levels to hypoxia. Rats susceptible to hypoxia are characterized by a more pronounced inflammatory response induced by LPS.

show abstract

Section: Introductionmentioning

confidence: 99%

<p>Dependence of the severity of the systemic inflammatory response on resistance to hypoxia in male Wistar rats</p>

Джалилова

Косырева

Diatroptov

et al. 2019

JIR

View full text Add to dashboard Cite

show abstract

“…Subsequently, DNA was washed to remove impurities and was eluted in TE buffer, as previously described (Guimaraes et al 2007 ). DNA from paraffin embedded tissue was extracted as previously described (Fraga et al 2012 ; de Carvalho Fraga et al 2013 ).…”

Section: Methodsmentioning

confidence: 99%

Leptin receptor polymorphism Gln223Arg (rs1137101) in oral squamous cell carcinoma and potentially malignant oral lesions

et al. 2014

Self Cite

View full text Add to dashboard Cite

The purpose of this study was to assess the LEPR gene Gln223Arg polymorphism (rs1137101) in oral squamous cell carcinoma (OSCC) and in potentially malignant oral lesions (PMOL) in comparison to normal oral mucosa in a Brazilian population. Smokers (n = 89) were selected from a representative sample of 471 individuals from the general population of Montes Claros, Brazil. Participants were age and gender matched to patients with OSCC (n = 25) and oral epithelial dysplasia (n = 25). We investigated the LEPR Gln223Arg polymorphism (A>G; rs1137101) in these groups. Genotype variants were assessed by RFLP-PCR, using MspI (HPAII) restriction endonuclease. The institutional review board of the Universidade Estadual de Montes Claros approved the study (process number 2667/2011). Written informed consent for this study was obtained from all participants. The GG genotype (Arg223Arg) appears to be the more relevant polymorphic variant in OSCC. It occurred, approximately, twice as frequently in OSCC patients than in the general population. In contrast, the A allele in its homozygosis form (Gln223Gln) is significantly associated with the development of PMOL; 80% of the samples from the PMOL group exhibit AA genotype. Our findings suggest new insights regarding LEPR gene variations in the development of OSCC and PMOL.

show abstract

“…This fact was evidenced [ 85 ] after the detection of the 582Ser allele (Pro/Ser heterozygotes) ratio in Polish weightlifters, which was significantly higher than in the control group. Nevertheless, a basic HIF-1α high expression level, provided by polymorphisms, can facilitate worse prognosis in some diseases, including tumors [ 81 , 86 , 87 , 88 , 89 ]. Tibetan-specific allelic variations in the EPAS1 gene ( HIF-2A ), regulating the physiological reactions to high-altitude hypoxia through the HIF signaling pathway to maintain the hemoglobin levels of Tibetan highlanders at near-sea level values, were revealed [ 90 ].…”

Section: Cellular Response To Hypoxic Exposurementioning

confidence: 99%

“…In experimental studies, according to the results of the survival time (the time before respiratory disturbances and signs of asphyxia appear) in an assessment under conditions of hypobaric hypoxia, animals are divided into tolerant and susceptible to oxygen deficiency. Humans are mostly divided into tolerant and susceptible groups according to their AMS and HAPE sensitivity [ 72 , 73 , 74 , 75 , 76 , 77 , 78 , 79 , 80 , 81 , 82 , 83 , 84 , 85 , 86 , 87 , 88 , 89 , 90 , 91 , 92 , 93 , 94 , 95 , 96 , 97 , 98 , 99 , 100 , 101 , 123 , 124 ].…”

Section: Physiological Biochemical and Molecular-biological Charmentioning

confidence: 99%

Differences in Tolerance to Hypoxia: Physiological, Biochemical, and Molecular-Biological Characteristics

Джалилова

Makarova

2020

Biomedicines

View full text Add to dashboard Cite

Hypoxia plays an important role in the development of many infectious, inflammatory, and tumor diseases. The predisposition to such disorders is mostly provided by differences in basic tolerance to oxygen deficiency, which we discuss in this review. Except the direct exposure of different-severity hypoxia in decompression chambers or in highland conditions, there are no alternative methods for determining organism tolerance. Due to the variability of the detection methods, differences in many parameters between tolerant and susceptible organisms are still not well-characterized, but some of them can serve as biomarkers of susceptibility to hypoxia. At the moment, several potential biomarkers in conditions after hypoxic exposure have been identified both in experimental animals and humans. The main potential biomarkers are Hypoxia-Inducible Factor (HIF)-1, Heat-Shock Protein 70 (HSP70), and NO. Due to the different mechanisms of various high-altitude diseases, biomarkers may not be highly specific and universal. Therefore, it is extremely important to conduct research on hypoxia susceptibility biomarkers. Moreover, it is important to develop a method for the evaluation of organisms’ basic hypoxia tolerance without the necessity of any oxygen deficiency exposure. This can contribute to new personalized medicine approaches’ development for diagnostics and the treatment of inflammatory and tumor diseases, taking into account hypoxia tolerance differences.

show abstract

High HIF-1α expression genotypes in oral lichen planus

Cited by 16 publications

References 46 publications

<p>Dependence of the severity of the systemic inflammatory response on resistance to hypoxia in male Wistar rats</p>

<p>Dependence of the severity of the systemic inflammatory response on resistance to hypoxia in male Wistar rats</p>

Leptin receptor polymorphism Gln223Arg (rs1137101) in oral squamous cell carcinoma and potentially malignant oral lesions

Differences in Tolerance to Hypoxia: Physiological, Biochemical, and Molecular-Biological Characteristics

Contact Info

Product

Resources

About