High hydrostatic pressure equipment for neutron scattering studies of samples in solutions

Peters, Judith; Trapp, Marcus; Hughes, Darren J.; Rowe, S.; Demé, Bruno; Laborier, Jean-Luc; Payre, Claude; Gonzales, Jean-Paul; Baudoin, Simon; Belkhier, Nadir; Lelièvre‐Berna, E.

doi:10.1080/08957959.2011.642376

Cited by 27 publications

(24 citation statements)

References 6 publications

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“…The samples were in solution to ensure a homogeneous transmission of hydrostatic pressure and to highlight the single particle dynamics. For the dynamical high pressure experiments, we took advantage of equipment developed and validated within the last years at the neutron scattering facility Institut Laue Langevin (ILL, Grenoble, France) for measurements of samples in solution34. We used the time-of-flight spectrometers IN535 and IN636 as well as the thermal backscattering spectrometer IN1337 at the ILL which permitted to combine elastic and quasi-elastic scattering investigations and to use two different time windows to identify various types of motions at the atomic scale.…”

Section: Methodsmentioning

confidence: 99%

High hydrostatic pressure specifically affects molecular dynamics and shape of low-density lipoprotein particles

Golub

Lehofer

Martínez

et al. 2017

Sci Rep

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Lipid composition of human low-density lipoprotein (LDL) and its physicochemical characteristics are relevant for proper functioning of lipid transport in the blood circulation. To explore dynamical and structural features of LDL particles with either a normal or a triglyceride-rich lipid composition we combined coherent and incoherent neutron scattering methods. The investigations were carried out under high hydrostatic pressure (HHP), which is a versatile tool to study the physicochemical behavior of biomolecules in solution at a molecular level. Within both neutron techniques we applied HHP to probe the shape and degree of freedom of the possible motions (within the time windows of 15 and 100 ps) and consequently the flexibility of LDL particles. We found that HHP does not change the types of motion in LDL, but influences the portion of motions participating. Contrary to our assumption that lipoprotein particles, like membranes, are highly sensitive to pressure we determined that LDL copes surprisingly well with high pressure conditions, although the lipid composition, particularly the triglyceride content of the particles, impacts the molecular dynamics and shape arrangement of LDL under pressure.

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Section: Methodsmentioning

confidence: 99%

High hydrostatic pressure specifically affects molecular dynamics and shape of low-density lipoprotein particles

Golub

Lehofer

Martínez

et al. 2017

Sci Rep

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“…A high pressure equipment dedicated to neutron scattering experiments of biological samples in solution has been developed over the last years at the ILL in Grenoble [14]. In brief, it consists of a high pressure stick, which can be placed in the cryostats or cryofurnaces of the instruments, a pressure controller which allows a remote control and permanent adjustment of pressure and cylindrical pressure containers built of a high-tensile aluminum alloy.…”

Section: High Pressure Equipmentmentioning

confidence: 99%

Deep Sea Microbes Probed by Incoherent Neutron Scattering Under High Hydrostatic Pressure

Peters¹,

Martínez²,

Michoud

et al. 2014

Zeitschrift Für Physikalische Chemie

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The majority of the biosphere is a high pressure environment. Around 70% of the marine biosphere lies at depths below 1000 m, i.e. at pressures of 100 bars or higher. To survive in these environments, deep-biosphere organisms have adapted to life at high pressure. In vitro studies showed that the activity of certain proteins originating from deep-sea organisms is less affected by high pressure than that of enzymes from surface organisms [1-3]. However, the genetic and structural bases for this increased pressure resistance are still unknown. Elastic incoherent neutron scattering studies, which provide access to information about molecular dynamics, constitute a very promising approach to decipher the structural adaptation in proteins living under high pressure. This approach has been used in the past to investigate the adaptation of biological systems to temperature and salinity and proved to be essential and complementary to structural studies. Here first investigations of high pressure effects on cell dynamics are presented using Thermococcales as models.

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“…For ENS experiments we used the high hydrostatic pressure equipment developed at the Institut Laue Langevin (ILL) in Grenoble (France) [32] for neutron scattering studies of powder and solution samples. The cylindrical cell, built of the high-tensile aluminium alloy (7049-T6), is 4 mm thick and can withstand pressure loads up to 1.5 kbar.…”

mentioning

confidence: 99%

Experimental Evidence for a Liquid-Liquid Crossover in Deeply Cooled Confined Water

et al. 2014

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High hydrostatic pressure equipment for neutron scattering studies of samples in solutions

Cited by 27 publications

References 6 publications

High hydrostatic pressure specifically affects molecular dynamics and shape of low-density lipoprotein particles

High hydrostatic pressure specifically affects molecular dynamics and shape of low-density lipoprotein particles

Deep Sea Microbes Probed by Incoherent Neutron Scattering Under High Hydrostatic Pressure

Experimental Evidence for a Liquid-Liquid Crossover in Deeply Cooled Confined Water

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