High hydrostatic pressure pre-treatment of whey proteins enhances whey protein hydrolysate inhibition of oxidative stress and IL-8 secretion in intestinal epithelial cells

Piccolomini, André F; Iskandar, Michèle M.; Lands, Larry C.; Kubow, Stan

doi:10.3402/fnr.v56i0.17549

Cited by 91 publications

(63 citation statements)

References 41 publications

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“…In this study and in the previous study by Piccolomini et al. (), modified milk proteins have been shown to suppress IL‐8 concentrations in cells already exhibiting inflammation. The anti‐inflammatory effects of fractions in this study indicate that the bioactive molecules within these fractions could be useful agents in the re‐establishment of GIT homeostasis, which may have been lost due to disregulated inflammation.…”

Section: Discussionsupporting

confidence: 76%

Anti‐inflammatory effects of a casein hydrolysate and its peptide‐enriched fractions on TNFα‐challenged Caco‐2 cells and LPS‐challenged porcine colonic explants

Mukhopadhya

Noronha

Bahar

et al. 2014

Food Science & Nutrition

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Bioactive milk peptides are reported to illicit a range of physiological benefits and have been proposed as potential functional food ingredients. The objective of this study was to characterize the anti-inflammatory properties of sodium caseinate (NaCAS), its enzyme hydrolysate (EH) and peptide-enriched fractions (5 kDa retentate [R], 1 kDaR and 1 kDa permeate [P]), both in vitro using a Caco-2 cell line, and also ex vivo using a porcine colonic tissue explant system. Caco-2 cells were stimulated with tumour necrosis factor alpha (TNFα) and co-treated with casein hydrolysates for 24 h. Following this, interleukin (IL)-8 concentrations in the supernatant were measured using enzyme-linked immunosorbent assay. Porcine colonic tissue was stimulated with lipopolysaccharide and co-treated with casein hydrolysates for 3 h. The expression of a panel of inflammatory cytokines was measured using qPCR. While dexamethasone reduced the IL-8 concentration by 41.6%, the 1 kDaR and 1 kDaP fractions reduced IL-8 by 68.7% and 66.1%, respectively, relative to TNFα-stimulated Caco-2 cells (P < 0.05). In the ex vivo system, only the 1 kDaR fraction elicited a decrease inIL1-α,IL1-β,IL-8,TGF-β andIL-10 expression (P < 0.05). This study provides evidence that the bioactive peptides present in the 1 kDaR fraction of the NaCAS hydrolysate possess anti-inflammatory properties in vitro and ex vivo. Further in vivo analysis of the anti-inflammatory properties of the 1 kDaR is proposed.

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Section: Discussionsupporting

confidence: 76%

Anti‐inflammatory effects of a casein hydrolysate and its peptide‐enriched fractions on TNFα‐challenged Caco‐2 cells and LPS‐challenged porcine colonic explants

Mukhopadhya

Noronha

Bahar

et al. 2014

Food Science & Nutrition

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“…30,31 Similarly, IL-8, which is produced by immune cells such as macrophages, endothelial cells and epithelial cells in the gut, is another established marker for inflammation of the gastrointestinal system. 33 The results of this study and previous research indicated that these milk hydrolysates may exert inhibitory effects on the NFκβ pathway or may actively re-establish homeostasis following inflammation e.g. 32 The 5kDaR co-treatment of TNF-α stimulated Caco-2 cells resulted in a reduction in IL-8 concentration.…”

Section: Discussionsupporting

confidence: 51%

The anti-inflammatory potential of a moderately hydrolysed casein and its 5 kDa fraction in in vitro and ex vivo models of the gastrointestinal tract

et al. 2015

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Bioactive peptides from milk can impart a wide range of physiological benefits without the allergies and intolerance associated with the consumption of whole milk. The objective of this study was to characterise the anti-inflammatory properties of intact sodium caseinate (NaCAS), a moderately hydrolysed NaCAS enzyme hydrolysate (EH) and its 5 kDa fraction (5kDaR), in both in vitro and ex vivo systems. In vitro, Caco-2 cells were stimulated with tumor necrosis factor (TNF) α and co-treated ± casein hydrolysates or dexamethasone (control). The inflammatory marker interleukin (IL)-8 was measured by ELISA in the supernatant at 24 h. Ex vivo, porcine colonic tissues were stimulated with lipopolysaccharide (LPS) and co-treated with casein hydrolysates for 3 h from which the relative expression of a panel of cytokines was measured in vitro. While the steroid dexamethasone brought about a 41.6% reduction in the IL-8 concentration in the supernatant, the 5kDaR reduced IL-8 by 59% (P < 0.05) when compared to the TNFα stimulated Caco-2 cells. In the ex vivo system, 5kDaR was associated with decreases in IL-1α, IL-1β, IL-8 and TGF-β expression and an increase in IL-17 expression (P < 0.05) relative to the LPS challenged tissues. We concluded, that a 5 kDa casein fraction demonstrates potent anti-inflammatory effects both in in vitro and ex vivo models of the gastrointestinal tract.

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“…As precursor of the antioxidant glutathione, whey protein holds eminese in nullifying the adverse effects of the stressors. Hyperbaric treatment of whey protein accelerated the release of bioactive peptides, raised intracellular glutathione level and abated the in vitro generation of interleukin IL-8 (this cytokine is believed to mediate pathogenesis of respiratory tract diseases) (Piccolomini et al 2012). It was observed that one month dietary supplementation with pressurized whey (20 g/day) in cystic fibrosis patients significantly reduced serum C-reactive protein (CRP level is the metric of inflammation in body) level (Lands et al 2010).…”

Section: Antioxidant Anti-inflammation and Hepato-protectionmentioning

confidence: 94%

“…The evidence supporting the role of whey protein in augmenting glutathione synthesis in neurons and alleviating neurodegenerative maladies was reviewed (Ross et al 2012). The antioxidant and anti-inflammatory effects of pressurized whey protein isolate and native hydrolysate in human epithelial colorectal adenocarcinoma Caco-2 cells exposed to H 2 O 2 were compared (Piccolomini et al 2012). Both the pressurized and native hydrolysate inhibited IL-8 secretion and reactive oxygen species (ROS) generation while increasing ferric ion reducing antioxidant power (FRAP) in a dose-dependent manner.…”

Section: Antioxidant Anti-inflammation and Hepato-protectionmentioning

confidence: 99%

Emerging trends in nutraceutical applications of whey protein and its derivatives

Patel

2015

J Food Sci Technol

130

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The looming food insecurity demands the utilization of nutrient-rich residues from food industries as valueadded products. Whey, a dairy industry waste has been characterized to be excellent nourishment with an array of bioactive components. Whey protein comprises 20 % of total milk protein and it is rich in branched and essential amino acids, functional peptides, antioxidants and immunoglobulins. It confers benefits against a wide range of metabolic diseases such as cardiovascular complications, hypertension, obesity, diabetes, cancer and phenylketonuria. The protein has been validated to boost recovery from resistance exercise-injuries, stimulate gut physiology and protect skin against detrimental radiations. Apart from health invigoration, whey protein has proved its suitability as fat replacer and emulsifier. Further, its edible and antimicrobial packaging potential renders its highly desirable in food as well as pharmaceutical sectors. Considering the enormous nutraceutical worth of whey protein, this review emphasizes on its established and emerging biological roles. Present and future scopes in food processing and dietary supplement formulation are discussed. Associated hurdles are identified and how technical advancement might augment its applications are explored. This review is expected to provide valuable insight on whey protein-fortified functional foods, associated technical hurdles and scopes of improvement.

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High hydrostatic pressure pre-treatment of whey proteins enhances whey protein hydrolysate inhibition of oxidative stress and IL-8 secretion in intestinal epithelial cells

Cited by 91 publications

References 41 publications

Anti‐inflammatory effects of a casein hydrolysate and its peptide‐enriched fractions on TNFα‐challenged Caco‐2 cells and LPS‐challenged porcine colonic explants

Anti‐inflammatory effects of a casein hydrolysate and its peptide‐enriched fractions on TNFα‐challenged Caco‐2 cells and LPS‐challenged porcine colonic explants

The anti-inflammatory potential of a moderately hydrolysed casein and its 5 kDa fraction in in vitro and ex vivo models of the gastrointestinal tract

Emerging trends in nutraceutical applications of whey protein and its derivatives

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