BackgroundHate crimes have raised in Spain and the gender and sexuality-based conflict persist worldwide which leads to this problem having an effect on health and wellbeing. Following a focus of transforming Higher Education Institutions (HEI), this research focusses on analysing how this problem affects undergraduate students in six Spanish universities. The goal of this study is to improve the quality of life of LGBTQI+ University students, breaking the silence that exists about the violence that this group suffer in Catalonia, Spain.MethodsFollowing the Communicative Methodology, this study has identified violence based on sexual orientation, gender identity or expression in the target universities and provided guidelines to improve anti-discrimination protocols. A qualitative method has reached experiences of university students, heads of equality commissions, professors and administrative staff regarding this conflict. Focussing on the qualitative research tools, 30 half-structured interviews were conducted with university students, staff and professors around issues related to LGBTQI-phobic violence: 1) perception of violence and discrimination, 2) institutional measures, 3) actions against violence. An analysis of exclusionary and transformative dimensions was used to identify emergent themes. ResultsWe have identified two dimensions for the analysis: exclusionary -those that maintain the problem-, and transformative, -those that contribute to transform the problem of LGBTQI-phobia-. On the one hand, exclusionary facts have been described by participants in the study as a wide range of forms of violence against the LGBTQI+ community perpetrated at university, mainly verbal and psychological. Moreover, equality commissions have not received reports of LGBTQI-phobic violence, and university staff and professors show certain unfamiliarity regarding measures and politics to prevent and intervene in cases of violence on the grounds of sexual orientation, gender identity and gender expression. On the other hand, in the study of variables that contribute to overcoming this conflict, actions of LGBTQI groups against violence and the professors’ commitment to intervene show relevant changes in student’s wellbeing. An improvement and implementation of anti-discrimination protocols with mandatory measures has also been documented.ConclusionsFindings highlight the need for evidence that contributes to the improvement of protocols, measures and politics to protect the LGBTQI community at university. A better understanding of violence based on sexual orientation, gender identity and gender expression in HEI’s may guide national and international governments to improve the LGBTQI collectives’ health and well-being. This study provides relevant information regarding this pressing challenge and presents an important impact achieved contributing to the improvement of the quality of life of the LGBTQI+ community.Trial registrationNot applicable