High incidence of multi-drug resistance and heterogeneity of mobile genetic elements in Escherichia coli isolates from diseased ducks in Sichuan province of China

Zhang, Shaqiu; Chen, Shu‐Ling; Abbas, Muhammad; Wang, Mingshu; Jia, Renyong; Chen, Shun; Liu, Mafeng; Zhu, Dekang; Zhao, Xinxin; Wu, Ying; Yang, Qiao; Huan, Juan; Ou, Xumin; Mao, Sai; Gao, Qun; Sun, Di; Tian, Bin; Cheng, Anchun

doi:10.1016/j.ecoenv.2021.112475

Cited by 13 publications

(16 citation statements)

References 51 publications

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“…Antibiotic sensitivity tests revealed that colistin-resistant E. coli isolates have a highly resistance to sulfisoxazole; a moderate rate of resistance to ampicillin, streptomycin, and tetracycline; and a low rate of resistance to cefuroxime, ceftazidime, cefepime, ceftriaxone, cefoxitin, aztreonam, ampicillin-sulbactam, amoxicillin-clavulanic acid, gentamicin, kanamycin, ciprofloxacin, and levofloxacin. The colistin-resistant E. coli isolates displayed high resistance rates to antibiotics that are commonly used in veterinary medicine in Sichuan Province ( Ma et al, 2017 ; Zhang et al, 2021 ), including sulfisoxazole, ampicillin, streptomycin, tetracycline, and chloramphenicol. The antimicrobial resistance rates have reached to 30%, which may be due to the breadth of our sample or because of the generally high use of antibiotics in Sichuan Province.…”

Section: Resultsmentioning

confidence: 99%

Molecular Epidemiology and Colistin-Resistant Mechanism of mcr-Positive and mcr-Negative Escherichia coli Isolated From Animal in Sichuan Province, China

Cheng

et al. 2022

Front. Microbiol.

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Colistin is the last line of defense for the treatment of multidrug-resistant gram-negative bacterial infections. However, colistin resistance is gradually increasing worldwide, with resistance commonly regulated by two-component system and mcr gene. Thus, this study aimed to investigate molecular epidemiology and colistin-resistant mechanism of mcr-positive and mcr-negative Escherichia coli isolates from animal in Sichuan Province, China. In this study, a total of 101 colistin-resistant E. coli strains were isolated from 300 fecal samples in six farms in Sichuan Province. PCR was used to detect mcr gene (mcr-1 to mcr-9). The prevalence of mcr-1 in colistin-resistant E. coli was 53.47% (54/101), and the prevalence of mcr-3 in colistin-resistant E. coli was 10.89% (11/101). The colistin-resistant E. coli and mcr-1–positive E. coli showed extensive antimicrobial resistance profiles. For follow-up experiments, we used 30 mcr-negative and 30 mcr-1–positive colistin-resistant E. coli isolates and E. coli K-12 MG1655 model strain. Multi-locus sequence typing (MLST) of 30 strains carrying mcr-1 as detected by PCR identified revealed six strains (20%) of ST10 and three strains (10%) of each ST206, ST48, and ST155 and either two (for ST542 and 2539) or just one for all other types. The conjugation experiment and plasmid replicon type analysis suggest that mcr-1 was more likely to be horizontally transferred and primarily localized on IncX4-type and IncI2-type plasmid. The ST diversity of the mcr-1 indicated a scattered and non-clonal spreading in mcr-1–positive E. coli. Twenty-eight mcr-negative colistin-resistant E. coli isolates carried diverse amino acid alterations in PmrA, PmrB, PhoP, PhoQ, and MgrB, whereas no mutation was found in the remaining isolates. The finding showed the high prevalence of colistin resistance in livestock farm environments in Sichuan Province, China. Our study demonstrates that colistin resistance is related to chromosomal point mutations including the two-component systems PhoP/PhoQ, PmrA/PmrB, and their regulators MgrB. These point mutations may confer colistin resistance in mcr-negative E. coli. These findings help in gaining insight of chromosomal-encoded colistin resistance in E. coli.

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Section: Resultsmentioning

confidence: 99%

Molecular Epidemiology and Colistin-Resistant Mechanism of mcr-Positive and mcr-Negative Escherichia coli Isolated From Animal in Sichuan Province, China

Cheng

et al. 2022

Front. Microbiol.

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“…Current measures have shown some effectiveness in preventing the presence of antibiotic resistant bacteria in animals and humans ( Tang et al, 2017 ). However, the prevalence of integrons as well as MGEs has been confirmed to be still high in China's poultry industry ( Mazhar et al, 2021 ; Zhang et al, 2021a ), potentially exacerbating the incidence of AMR. Hence, monitoring MGEs and integrons is essential for understanding the occurrence of AMR.…”

Section: Introductionmentioning

confidence: 99%

High rate of multidrug resistance and integrons in Escherichia coli isolates from diseased ducks in select regions of China

et al. 2023

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“…Among 165 E. coli strains isolated from duck farms in China's Sichuan province, 163 (98.8%) exhibited MDR. Most isolates revealed high resistance to ampicillin (95.8%), chloramphenicol (89.7%), and trimethoprim-sulfamethoxazole (84.2%) [40]. These results indicate that controlling multi-drug resistance in Enterobacteriaceae in duck husbandry requires particular vigilance.…”

Section: Discussionmentioning

confidence: 89%

“…These resistance genes might be transferred from livestock to humans through the food chain. Due to the prevalence of waterfowl bacterial infections and the extensive use of antibiotics, the spreading of MDR Enterobacteriaceae containing MGEs has accelerated in duck farms and industry [40].…”

Section: Discussionmentioning

confidence: 99%

Mobile genetic elements facilitate the transmission of antibiotic resistance genes in multidrug-resistant Enterobacteriaceae from duck farms

Zheng¹,

Xu²,

Yan³

et al. 2024

Food Science and Human Wellness

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Multidrug-resistant (MDR) Enterobacteriaceae critically threaten duck farming and public health. The phenotypes, genotypes, and associated mobile genetic elements (MGEs) of MDR Enterobacteriaceae isolated from six duck farms in Zhejiang province, China, were investigated. A total of 215 isolates were identified as Escherichia coli (64.65%), Klebsiella pneumoniae (12.09%), Proteus mirabilis (10.23%), Salmonella (8.84%), and Enterobacter cloacae (4.19%). Meanwhile, all isolates were resistant to at least two antibiotics. Most isolates carried tet(A) (85.12%), bla TEM (78.60%) and sul1 (67.44%) resistance genes. Gene co-occurrence analysis showed that the resistance genes were associated with IS26 and integrons. A conjugative IncFII plasmid pSDM004 containing all the above MGEs was detected in Proteus mirabilis isolate SDM004. This isolate was resistant to 18 antibiotics and carried the bla NDM-5 gene. MGEs, especially plasmids, are the primary antibiotic resistance gene (ARG) transmission route in duck farms. These findings provide a theoretical basis for the rational use of antibiotics in farms which are substantial for evaluating public health and food safety.

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High incidence of multi-drug resistance and heterogeneity of mobile genetic elements in Escherichia coli isolates from diseased ducks in Sichuan province of China

Cited by 13 publications

References 51 publications

Molecular Epidemiology and Colistin-Resistant Mechanism of mcr-Positive and mcr-Negative Escherichia coli Isolated From Animal in Sichuan Province, China

Molecular Epidemiology and Colistin-Resistant Mechanism of mcr-Positive and mcr-Negative Escherichia coli Isolated From Animal in Sichuan Province, China

High rate of multidrug resistance and integrons in Escherichia coli isolates from diseased ducks in select regions of China

Mobile genetic elements facilitate the transmission of antibiotic resistance genes in multidrug-resistant Enterobacteriaceae from duck farms

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