Mason, MR, Heebner, NR, Abt, JP, Bergstrom, HC, Shapiro, R, Langford, EL, and Abel, MG. The acute effect of high-intensity resistance training on subsequent firefighter performance. J Strength Cond Res 37(7): 1507–1514, 2023—High-intensity resistance training (HIRT) is commonly performed by structural firefighters on duty to enhance occupational readiness. However, exercise-induced fatigue may negatively impact subsequent occupational performance. Therefore, the purpose of this study was to assess the acute effect of HIRT on subsequent occupational physical ability in structural firefighters and to determine the time course of recovery. Seven resistance-trained male firefighters performed a timed maximal effort simulated fireground test (SFGT) in 3 randomized conditions: baseline (SFGTbaseline), 10 minutes post HIRT (SFGT10min), and 60 minutes post HIRT (SFGT60min). Work efficiency and air depletion were assessed during the SFGT. The timed HIRT session consisted of a standardized set of exercises using absolute training loads. Repeated measures analysis of variance and minimal difference (MD) analysis assessed group and individual effects between the conditions, respectively. The level of significance was set at p < 0.05. SFGT10min completion time was greater than SFGTbaseline (430 ± 137 vs. 297 ± 69 seconds, p < 0.01), with no difference between SFGTbaseline and SFGT60min (297 ± 69 vs. 326 ± 89 seconds, p = 0.08). The MD analysis indicated that all firefighters' SFGT10min times exceeded the MD (±26.4 seconds) compared with SFGTbaseline. However, 43% of firefighters still exceeded the MD at SFGT60min. Air depletion during SFGT10min was greater than that during SFGTbaseline (2,786 ± 488 vs. 2,186 ± 276 lb·in−2, p = 0.02), with no difference between SFGTbaseline and SFGT60min (p = 0.25). Work efficiency during SFGT10min was 40% lower than that during SFGTbaseline (p < 0.01), with no difference between SFGTbaseline and SFGT60min (p = 0.25). These findings indicate that an acute bout of HIRT decreases multiple descriptors of firefighters' occupational performance 10 minutes post exercise with varied responses at 60 minutes post exercise.