High-intensity interval training-induced hypertrophy in gastrocnemius muscle via improved IGF-I/Akt/FoxO and myostatin/Smad signaling pathways in rats

Biglari, Soheil; Afousi, Alireza Ghardashi; Mafi, Farnoosh; Shabkhiz, Fatemeh

doi:10.1556/2060.2020.00020

Cited by 16 publications

(8 citation statements)

References 28 publications

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“…However, the difference between MICT and Control seems to be mainly related to the grip strength decline in Control rather than an effect of MICT. Our results were in line with previous findings in healthy rats indicating a greater grip strength following HIIT compared with MICT due to higher muscle activation and/or muscle hypertrophy during HIIT sessions [45,46]. Similarly, both training regimens seemed to maintain motor coordination and tactile sensitivity by avoiding a decrease of mechanical sensitivity as observed in Control.…”

Section: Hiit and Mict Could Prevent The Decline In The Force Motor Coordination And Tactile Sensitivity Over Timesupporting

confidence: 92%

High-intensity interval training is superior to moderate intensity training on aerobic capacity in rats: Impact on hippocampal plasticity markers

Constans

Pin-Barre

Molinari

et al. 2021

Behavioural Brain Research

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This study was designed to compare work-matched high-intensity interval training (HIIT) with moderate-intensity continuous training (MICT) based on lactate threshold on aerobic performance, brain plasticity markers and cognitive functions following 8 weeks in healthy rats. Muscular plasticity and grip strength were also investigated. Rats performed the incremental exercise test and behavioural tests before (PRE) and after training at day 1 (D1), D15, D29 and D57. Key cerebral markers were assessed by Western blot and quantitative polymerase chain reaction to provide information on brain function related to angiogenesis, aerobic metabolism and neurotrophin activity at D59. Muscular protein levels involved in angiogenesis and aerobic metabolism were measured in both triceps brachii and soleus muscles.HIIT induced superior improvement of aerobic fitness compared to work-matched MICT, as indicated by enhancement of speed associated with lactate threshold (SLT) and maximal speed (Smax). In parallel, grip strength increased throughout the HIIT protocol. In the triceps brachii muscles, markers of angiogenesis and aerobic activity were upregulated as well as myokine involved in neuroplasticity. Moreover, levels of key brain plasticity markers increased in the hippocampus only following 8 weeks of HIIT, without improving cognitive functions.These findings might contribute to define physical exercise guidelines for maintaining brain health by highlighting the promising role of HIIT when using SLT for distinguishing low running speed from high running speed. Further studies are required to confirm these brain effects by exploring synaptic plasticity and neurogenesis mechanisms when exercise intensity is standardized and individualized.

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Section: Hiit and Mict Could Prevent The Decline In The Force Motor Coordination And Tactile Sensitivity Over Timesupporting

confidence: 92%

High-intensity interval training is superior to moderate intensity training on aerobic capacity in rats: Impact on hippocampal plasticity markers

Constans

Pin-Barre

Molinari

et al. 2021

Behavioural Brain Research

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“…Conditional FoxO1 ablation in skeletal muscle results in increased formation of MyoDcontaining fast fibers and altered fiber type distribution at the expense of myogenin-containing slow fibers (42). Decreased expression of FoxO1 in the gastrocnemius muscle after HIIT has already been shown in rats (43). Remarkably, we detected muscle FoxO1 down-regulation only in trained mice, which may indicate that this adaptation is dependent on the direct effect of exercise and not mediated by exosomal miRNAs.…”

Section: Discussionsupporting

confidence: 61%

Delivery of muscle-derived exosomal miRNAs induced by HIIT improves insulin sensitivity through down-regulation of hepatic FoxO1 in mice

Castaño

Mirasierra

Vallejo

et al. 2020

Proc. Natl. Acad. Sci. U.S.A.

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Implementation of regular physical activity helps in the maintenance of a healthy metabolic profile both in humans and mice through molecular mechanisms not yet completely defined. Here, we show that high-intensity interval training (HIIT) modifies the microRNA (miRNA) profile of circulating exosomes in mice, including significant increases inmiR-133aandmiR-133b. Importantly, treatment of sedentary mice with exosomes isolated from the plasma of trained mice improves glucose tolerance, insulin sensitivity, and decreases plasma levels of triglycerides. Moreover, exosomes isolated from the muscle of trained mice display similar changes in miRNA content, and their administration to sedentary mice reproduces the improvement of glucose tolerance. Exosomal miRNAs up-regulated by HIIT target insulin-regulated transcription factor forkhead box O1 (FoxO1) and, accordingly, expression ofFoxO1is decreased in the liver of trained and exosome-treated mice. Treatment with exosomes transfected with amiR-133bmimic or with a specific siRNA targetingFoxO1recapitulates the metabolic effects observed in trained mice. Overall, our data suggest that circulating exosomes released by the muscle carry a specific miRNA signature that is modified by exercise and induce expression changes in the liver that impact whole-body metabolic profile.

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“…The SED-HMB group (with injury) showed an increase in the TA CSA compared with the SED groups and, in both injured groups there was a significant increase in IGF-1 and p-Akt levels compared to the non-injured groups. IGF-1/Akt not only contributes to muscle regeneration (18,19), but is also associated with skeletal muscle hypertrophy (20,21), thus corroborating our findings of IGF-1/Akt pathway activation.…”

Section: Discussionsupporting

confidence: 89%

Beta-hydroxy-beta-methylbutyrate associated with low-intensity exercise training improves skeletal muscle regeneration through the IGF-Akt pathway

Yamada

Ferretti

Matsumura

et al. 2022

Braz J Med Biol Res

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The effect of beta-hydroxy-beta-methylbutyrate (HMB) supplementation associated with exercise training at different intensities and frequencies on skeletal muscle regeneration of muscle-injured rats was investigated. Male Wistar rats were divided into sedentary and trained groups. The sedentary groups were subdivided into non-injured (SED-Ct), non-injured supplemented with HMB (SED-Ct-HMB), injured (SED), and injured with HMB (SED-HMB), and the trained groups were injured, supplemented with HMB, and then divided into training three times a week without load (HT3) or with load (HT3L) and training five times a week without load (HT5) and with load (HT5L). The rats received a daily dose of HMB associated with 60 min of swimming with or without 5% body mass load for 14 days. On the 15th day, cryoinjury was performed in the right tibialis anterior muscle (TA), and 48 h later, supplementation and training continued for 15 days. After the last session, the TA was dissected and a cross-sectional area (CSA) of muscle fibers was used to determine the percentage of CSA fibers and connective tissue (%CT), as well as the total and phosphorylated protein contents. SED-HMB showed increased CSA and decreased %CT and TGF-b when compared to SED. HT3 showed increased CSA and reduced %CT accompanied by increased IGF-1/Akt, myogenin, and MuRF1, and decreased TGF-b. The CSA of HT5L also increased, but at the cost of a higher %CT compared to the other groups. Our results demonstrated that HMB associated with training without load and with lower frequency per week may be a valuable strategy for skeletal muscle regeneration.

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High-intensity interval training-induced hypertrophy in gastrocnemius muscle via improved IGF-I/Akt/FoxO and myostatin/Smad signaling pathways in rats

Cited by 16 publications

References 28 publications

High-intensity interval training is superior to moderate intensity training on aerobic capacity in rats: Impact on hippocampal plasticity markers

High-intensity interval training is superior to moderate intensity training on aerobic capacity in rats: Impact on hippocampal plasticity markers

Delivery of muscle-derived exosomal miRNAs induced by HIIT improves insulin sensitivity through down-regulation of hepatic FoxO1 in mice

Beta-hydroxy-beta-methylbutyrate associated with low-intensity exercise training improves skeletal muscle regeneration through the IGF-Akt pathway

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