High-intensity ultrasound: A novel technology for the development of probiotic and prebiotic dairy products

Guimarães, Jonas T.; Balthazar, Celso F.; Scudino, Hugo; Pimentel, Tatiana Colombo; Esmerino, Erick A.; Ashokkumar, Muthupandian; Freitas, Mônica Q.; Cruz, Adriano G.

doi:10.1016/j.ultsonch.2019.05.004

Cited by 123 publications

(81 citation statements)

References 62 publications

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“…Non-thermal treatments such as ultrasound treatment have been actively explored in recent years, as it involves minimal processing (Abbasi et al, 2019;Balthazar et al, 2019;Bhavya & Hebbar, 2019;Guimarães et al, 2019b;Mohd Khairi et al, 2018;Soltani et al, 2018). Minimal processing would significantly help to retain several nutritional and antioxidant properties of the product (Fernández-Barbero et al, 2019;Guimarães et al, 2019a;Guimarães et al, 2019b). This method may reduce the microbiological presence by manipulating the pressure changes, resulting in high shearing effects and high localized temperature (Guimarães et al, 2019a;Piyasena et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

“…Minimal processing would significantly help to retain several nutritional and antioxidant properties of the product (Fernández-Barbero et al, 2019;Guimarães et al, 2019a;Guimarães et al, 2019b). This method may reduce the microbiological presence by manipulating the pressure changes, resulting in high shearing effects and high localized temperature (Guimarães et al, 2019a;Piyasena et al, 2003). Thus, ultrasound treatment may be an effective alternative to thermal treatments on sugarcane juice.…”

Section: Introductionmentioning

confidence: 99%

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The effect of sonication and heat treatment on the physicochemical, nutritional and microbiological properties of different sugarcane variants

et al. 2020

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Red sugarcane (RS) is a lesser bred of sugarcane variant in Malaysia compared to yellow sugarcane (YS), thus less data is available regarding this species. On this study, the physicochemical and microbiological properties of RS was determined and compared with YS under different conditions. Both sugarcane variants were subjected to heat and sonication treatments for 5 and 15 minutes as a method for preservation. RS contains a higher °Brix (15) compared to YS (14), and was unchanged while YS demonstrated a significant reduction. Furthermore, RS displayed a superior fructose (+802%), glucose (+119%) and sucrose (+17.91%) levels in all samples and they were largely preserved during both treatments. However, RS was sensitive to colour changes, as darker juice (decrease in lightness and increase in redness) was produced after treatments. RS possessed lower phenolic content (-31.15%) and antioxidant activity (-11.51%) than YS, and was significantly affected by both treatments. Sonication was more effective in reducing the microbial content than heat in both treatments. These findings indicated that RS is better at retaining its physicochemical properties under preservation treatment, but less resistant in preserving its appearance and nutritional values.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The effect of sonication and heat treatment on the physicochemical, nutritional and microbiological properties of different sugarcane variants

et al. 2020

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“…We reasoned that delivering Cry5B in a live bacterium (Bt or otherwise) to humans would be problematic given that 1) Bt is closely related to Bacillus cereus, which can cause food poisoning, 2) release of live recombinant bacteria has environmental concerns because live bacteria in the soil could select for Cry5B resistance against free living stages of hookworms, 3) live bacteria could replicate in the human gastrointestinal tract (amplifying environmental and resistance concerns), 4) there are uncertainties and significant safety concerns in the response of billions of people to live bacteria, 5) degradation of live bacteria during storage could reduce potency and stability, and 6) delivery of a stable live bacterial therapeutic around the world for mass drug administration would be difficult to achieve (36)(37)(38)(39)(40). An alternative of producing and delivering Cry5B as a purified protein is also problematic as making enough purified protein cheaply and massively for mass drug administration is difficult to envision.…”

Section: Downloaded Frommentioning

confidence: 99%

Recombinant Paraprobiotics as a New Paradigm for Treating Gastrointestinal Nematode Parasites of Humans

Abraham

Gazzola

et al. 2021

Antimicrob Agents Chemother

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Gastrointestinal nematodes (GINs) of humans, e.g., hookworms, negatively impact childhood growth, cognition, nutrition, educational attainment, income, productivity, and pregnancy. Hundreds of millions of people are targeted with mass drug administration (MDA) of donated benzimidazole anthelmintics. However, benzimidazole efficacy against GINs is suboptimal, and reduced/low efficacy has been seen. Developing an anthelmintic for human MDA is daunting: it must be safe, effective, inexpensive, stable without a cold chain, and massively scalable. Bacillus thuringiensis (Bt) crystal protein 5B (Cry5B) has anthelmintic properties that could fill this void. Here we develop an Active Pharmaceutical Ingredient or API containing Bt Cry5B compatible with MDA. We expressed Cry5B in asporogenous Bt during vegetative phase, forming cytosolic crystals. These Bacteria with Cytosolic Crystals (BaCC) were rendered inviable (inactivated BaCC or IBaCC) with food-grade essential oils. IBaCC potency was validated in vitro against nematodes. IBaCC was also potent in vivo against human hookworm infections in hamsters. IBaCC production was successfully scaled to 350 liters at a contract manufacturing facility. A simple fit-for-purpose formulation to protect against stomach digestion and powdered IBaCC were successfully made and used against GINS in hamsters and mice. A pilot histopathology study and blood chemistry workup showed that five daily consecutive doses of 200 mg/kg Cry5B IBaCC (the curative single dose is 40 mg/kg) was non-toxic to hamsters and completely safe. IBaCC is a safe, inexpensive, highly effective, easy-to-manufacture, and scalable anthelmintic that is practical for MDA and represents a new paradigm for treating human GINs.

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“…It is the first line of defense for cells to be persecuted by the external environment or treatment. To determine whether ultrasonic treatment affects the integrity of the C. Therefore, ultrasound process parameters must be truly quantified and controlled to achieve the desired level of cell permeability [25,41,42]. In addition to direct microbubble contact, microstreaming around cavitating microbubbles provides a second possible origin of mechanical stress on the cellular membrane influencing permeability [41].…”

Section: Effect Of Ultrasonic Treatment On Cell Permeabilitymentioning

confidence: 99%

Effect of Ultrasound on L-leucine Production by Corynebacterium Glutamicum in Fed-batch Culture

Zhang

Chen

Sun

et al. 2020

Preprint

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Background: Various process intensifications are proposed to improve process efficiency, quality, and safety of fermented products. With the market significantly growing, the production process of L-leucine needs significant improvements to increased productivity and yield. Ultrasound, a process intensification, is an emerging and fast-growing technology due to its wide range of applications in food science and fermentation industry. Results: By optimizing the time points and parameters of ultrasound, the biomass of Corynebacterium glutamicum CP was significantly improved. The response surface methodology was adopted to create prediction profiler model and optimize sonication conditions. C. glutamicum CP fermentation at conditions of ultrasonic power of 94 W/L, frequency of 25 kHz, interval of 31 min, and duration of 37 s produced 52.89 g/L of L-leucine in 44 h, increased by 21.6% compared with the control. The production performance of L-leucine was also improved under ultrasonic treatment. Moreover, the cell morphology of C. glutamicum CP were more irregular, elongated, and swollen cells under the ultrasonic condition. The cell permeability of C. glutamicum CP and the activity of key enzymes with ultrasonic treatment were significantly higher than the control. Conclusions: We successfully obtain the optimum ultrasonic conditions to produce L-leucine in fed-batch culture. The results indicate that ultrasonication is an efficient intensification process for enhancing fermentation to produce L-leucine from C. glutamicum CP.

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High-intensity ultrasound: A novel technology for the development of probiotic and prebiotic dairy products

Cited by 123 publications

References 62 publications

The effect of sonication and heat treatment on the physicochemical, nutritional and microbiological properties of different sugarcane variants

The effect of sonication and heat treatment on the physicochemical, nutritional and microbiological properties of different sugarcane variants

Recombinant Paraprobiotics as a New Paradigm for Treating Gastrointestinal Nematode Parasites of Humans

Effect of Ultrasound on L-leucine Production by Corynebacterium Glutamicum in Fed-batch Culture

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