High internal phase emulsion templating as a route to well-defined porous polymers

Cameron, Neil R.

doi:10.1016/j.polymer.2004.11.097

Cited by 679 publications

(625 citation statements)

References 29 publications

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“…The surface porosity of the polyHIPE is dependent on the material that the HIPE is in contact with during polymerisation. 24 This investigation found that tailored porous microspheres were able to support hES-MP cell growth over 11 days in culture. Microspheres were found to aggregate over time as cells and extracellular materials were observed binding the microspheres together.…”

Section: Discussionmentioning

confidence: 85%

“…Indeed, the surface porosity of polyHIPEs is highly affected when curing them on different surface energy materials. 24 Surface destabilisation of the emulsion could be occurring at the emulsion syringe interface depending on the surface energy, where a preferential wettability of the oil phase may be creating a thin monomer film around the HIPE droplet, resulting in a surface skin upon polymerisation. The surface porosity of the polyHIPE is dependent on the material that the HIPE is in contact with during polymerisation.…”

Section: Discussionmentioning

confidence: 99%

“…22 PolyHIPE fabrication methods are of particular interest because of the extremely high interconnected porosity achievable with this system. PolyHIPEs (polymers with an open porosity greater than 74% of the total internal volume) 23,24 can be fashioned into porous microspheres via a double emulsion. 25 The HIPE emulsion is produced by the dropwise addition of the internal phase to a continuous phase.…”

Section: -2877/2018/2(2)/026103/19mentioning

confidence: 99%

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Porous microspheres support mesenchymal progenitor cell ingrowth and stimulate angiogenesis

et al. 2018

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Porous microspheres have the potential for use as injectable bone fillers to obviate the need for open surgery. Successful bone fillers must be able to support vascularisation since tissue engineering scaffolds often cease functioning soon after implantation due to a failure to vascularise rapidly. Here, we test the angiogenic potential of a tissue engineered bone filler based on a photocurable acrylate-based high internal phase emulsion (HIPE). Highly porous microspheres were fabricated via two processes, which were compared. One was taken forward and investigated for its ability to support human mesenchymal progenitor cells and angiogenesis in a chorioallantoic membrane (CAM) assay. Porous microspheres with either a narrow or broad size distribution were prepared via a T-junction microfluidic device or by a controlled stirred-tank reactor of the HIPE water in oil in water (w/o/w), respectively. Culture of human embryonic stem cell-derived mesenchymal progenitor (hES-MP) cells showed proliferation over 11 days and formation of cell-microsphere aggregates. In-vitro , hES-MP cells were found to migrate into microspheres through their surface pores over time. The presence of osteoblasts, differentiated from the hES-MP cells, was evidenced through the presence of collagen and calcium after 30 days. Microspheres pre-cultured with cells were implanted into CAM for 7 days and compared with control microspheres without pre-cultured cells. The hES-MP seeded microspheres supported greater angiogenesis, as measured by the number of blood vessels and bifurcations, while the empty scaffolds attracted host chick cell ingrowth. This investigation shows that controlled fabrication of porous microspheres has the potential to create an angiogenic, bone filling material for use as a cell delivery vehicle.

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Section: Discussionmentioning

confidence: 85%

Section: Discussionmentioning

confidence: 99%

Section: -2877/2018/2(2)/026103/19mentioning

confidence: 99%

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Porous microspheres support mesenchymal progenitor cell ingrowth and stimulate angiogenesis

et al. 2018

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“…Emulsion templating is a convenient method to prepare highly porous polymeric materials with well-defined morphology [1][2][3][4][5][6] . The process involves preparing a high internal phase emulsion (HIPE), i.e.…”

Section: Introductionmentioning

confidence: 99%

Degradable emulsion-templated scaffolds for tissue engineering from thiol–ene photopolymerisation

et al. 2012

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Further information on publisher's website:http://dx.doi.org/10.1039/c2sm26250aPublisher's copyright statement:Additional information: Use policyThe full-text may be used and/or reproduced, and given to third parties in any format or medium, without prior permission or charge, for personal research or study, educational, or not-for-prot purposes provided that:• a full bibliographic reference is made to the original source • a link is made to the metadata record in DRO • the full-text is not changed in any way The full-text must not be sold in any format or medium without the formal permission of the copyright holders.Please consult the full DRO policy for further details. AbstractEmulsion templating has been used to prepare highly porous polyHIPE materials by thiol-ene photoinitiated network formation. Commercially available multifunctional thiols and acrylates were formulated into water-in-oil high internal phase emulsions (HIPEs) using an appropriate surfactant, and the HIPEs were photo-cured. The temperature of the HIPE aqueous phase was found to influence the morphology of the resulting materials. In agreement with previous work, a higher aqueous phase temperature (80 o C) gave rise to a larger mean void and interconnect diameter. The influence of temperature on morphology was found to be reduced at higher porosity, but still significant. The Young's modulus of the porous materials was shown to be related to the functionality of the acrylate comonomer used. A mixture of penta-and hexa-acrylate gave rise to a 100-fold increase in modulus, compared to an analogous tri-functional acrylate. The materials could be functionalised conveniently by addition of mono-acrylates or thiols to the organic phase of the precursor HIPE. Degradation was observed to occur at a rate depending on the degradation conditions. Under cell culture conditions at 37 o C, 19% mass loss occurred over 15 weeks. The scaffolds were found to be capable of supporting the growth of keratinocytic cells (HaCaTs) over 11 days in culture. Some penetrative in-growth of the cells into the scaffold was observed.3

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“…Moreover, since the compartments can be packed tightly together and are separated by a thin polymer bilayers, polymersomes with large number of compartments can achieve a very high internal phase volume fraction; thus, such polymersomes have properties similar to high-internal-phase emulsions (HIPE), and can serve as templates for synthesis of highly porous polymers, known as polyHIPE 3 , with potential as low-weight materials 4 , scaffolds in tissue engineering 5,6 , sensor materials 7 as well as supports for solid phase synthesis 8 and for hydrogen storage 9 .…”

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confidence: 99%

Multicompartment polymersome gel for encapsulation

Shum

Weitz

2011

Soft Matter

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AbtractWe introduce an approach that combines the concepts of emulsion-templating and dewetting for fabricating polymersomes with a large number of compartments. The resultant polymersome gel behaves as a gel-like solid, but is a true vesicle suspended in an aqueous environment. Due to the thin membranes that separate the compartments, the polymersome gels have a high volume fraction of internal phase for encapsulation of hydrophilic actives; they also provide large surface area of diblock copolymer membrane for encapsulation of lipophilic actives. Multiple actives can also be encapsulated in the gel without crosscontamination. Our technique represents a simple and versatile bulk approach for fabricating polymersome gels; it does not require the use of any specialized equipment or subsequent polymerization steps to solidify the gel. The resultant polymersome gel is promising as an encapsulating structure as well as scaffold for tissue engineering.Polymer vesicles or polymersomes are capsules with shells consisting of bilayers of amphiphilic block copolymers.

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High internal phase emulsion templating as a route to well-defined porous polymers

Cited by 679 publications

References 29 publications

Porous microspheres support mesenchymal progenitor cell ingrowth and stimulate angiogenesis

Porous microspheres support mesenchymal progenitor cell ingrowth and stimulate angiogenesis

Degradable emulsion-templated scaffolds for tissue engineering from thiol–ene photopolymerisation

Multicompartment polymersome gel for encapsulation

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