High Iron-Sequestrating Bifidobacteria Inhibit Enteropathogen Growth and Adhesion to Intestinal Epithelial Cells In vitro

Vazquez-Gutierrez, Pamela; Wouters, Tomás de; Werder, Julia; Chassard, Christophe; Lacroix, Christophe

doi:10.3389/fmicb.2016.01480

Cited by 48 publications

(26 citation statements)

References 68 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Bifidobacterium kashiwanohense was highly prevalent (99%) and abundant (3.84-log rpm per stool) in the stools and was also negatively correlated with gut inflammation (r 2 = −0.2). This bacterium possesses high iron sequestration properties and might produce indole-3-lactic acids, which was shown to be a successful strategy to inhibit and compete with enteric pathogens (i.e., Salmonella and E. coli) (64,65). However, B. kashiwanohense was also positively correlated (r 2 = 0.21) with Campylobacter in our study, suggesting that the modulation of B. kashiwanohense may reduce the impact of pathobiont on the intestinal homeostasis but facilitates the persistence of Campylobacter in the child intestinal tract.…”

Section: Discussionmentioning

confidence: 99%

Co-occurrence of Campylobacter Species in Children From Eastern Ethiopia, and Their Association With Environmental Enteric Dysfunction, Diarrhea, and Host Microbiome

Terefe

Deblais

Ghanem

et al. 2020

Front. Public Health

View full text Add to dashboard Cite

High Campylobacter prevalence during early childhood has been associated with stunting and environmental enteric dysfunction (EED), especially in low resource settings. This study assessed the prevalence, diversity, abundance, and co-occurrence of Campylobacter spp. in stools from children in a rural area of eastern Ethiopia and their association with microbiome, diarrhea, and EED in children. Stool samples (n = 100) were collected from randomly selected children (age range: 360-498 days) in five kebeles in Haramaya District, Ethiopia. Diarrhea, compromised gut permeability, and gut inflammation were observed in 48, 45, and 57% of children, respectively. Campylobacter prevalence and species diversity were assessed using PCR and meta-total RNA sequencing (MeTRS). The prevalence of Campylobacter spp. in the children's stools was 50% (41-60%) by PCR and 88% (80-93.6%) by MeTRS (P < 0.01). Further, seven Campylobacter species (Campylobacter jejuni, Campylobacter upsaliensis, Campylobacter hyointestinalis, Campylobacter coli, Campylobacter sp. RM6137, uncultured Campylobacter sp., and Campylobacter sp. RM12175) were detected by MeTRS in at least 40% of children stools in high abundance (>1.76-log read per million per positive stool sample). Four clusters of Campylobacter species (5-12 species per cluster) co-occurred in the stool samples, suggesting that Campylobacter colonization of children may have occurred through multiple reservoirs or from a reservoir in which several Campylobacter species may co-inhabit. No associations between Campylobacter spp., EED, and diarrhea were detected in this cross-sectional study; however, characteristic microbiome profiles were identified based on the prevalence of Campylobacter spp., EED severity, and diarrhea. Forty-seven bacterial species were correlated with Campylobacter, and 13 of them also correlated with gut permeability, gut inflammation and/or EED severity. Forty-nine species not correlated with Campylobacter Terefe et al. Campylobacter and Microbiome in Stools were correlated with gut permeability, gut inflammation, EED severity and/or diarrhea. This study demonstrated that (1) in addition to C. jejuni and C. coli, multiple nonthermophilic Campylobacter spp. (i.e., Campylobacter hyointestinalis, Campylobacter fetus, and Campylobacter concisus) were frequently detected in the children's stools and (2) the Campylobacter, gut permeability, gut inflammation, EED severity, and diarrhea were associated with characteristic microbiome composition. Additional spatial and longitudinal studies are needed to identify environmental reservoirs and sources of infection of children with disparate Campylobacter species and to better define their associations with EED in low-income countries.

show abstract

Section: Discussionmentioning

confidence: 99%

Co-occurrence of Campylobacter Species in Children From Eastern Ethiopia, and Their Association With Environmental Enteric Dysfunction, Diarrhea, and Host Microbiome

Terefe

Deblais

Ghanem

et al. 2020

Front. Public Health

View full text Add to dashboard Cite

show abstract

“…Bifidobacteria are Gram-positive gut commensals of various mammals, insects and birds, where their presence is associated with a number of beneficial effects ( Ventura et al, 2014 ). Such beneficial effects include pathogen inhibition ( Fukuda et al, 2011 ; Vazquez-Gutierrez et al, 2016 ), immune modulation ( Fanning et al, 2012 ; Turroni et al, 2013 ), reduction in the symptoms of irritable bowel syndrome ( Whorwell et al, 2006 ) and cholesterol reduction ( Zanotti et al, 2015 ). In humans, bifidobacteria are particularly abundant and prevalent in the infant gut, though their relative abundance reduces upon weaning and upon ageing of their host ( Turroni et al, 2012 ).…”

Section: Introductionmentioning

confidence: 99%

Characterisation of a Hydroxycinnamic Acid Esterase From the Bifidobacterium longum subsp. longum Taxon

et al. 2018

View full text Add to dashboard Cite

Bifidobacterium longum subsp. longum, a common member of the human gut microbiota with perceived positive health effects, is capable of metabolising certain complex, plant-derived carbohydrates which are commonly found in the (adult) human diet. These plant glycans may be employed to favourably modulate the microbial communities in the intestine. Hydroxycinnamic acids (HCAs) are plant phenolic compounds, which are attached to glycans, and which are associated with anti-oxidant and other beneficial properties. However, very little information is available regarding metabolism of HCA-containing glycans by bifidobacteria. In the current study, a gene encoding a hydroxycinnamic acid esterase was found to be conserved across the B. longum subsp. longum taxon and was present in a conserved locus associated with plant carbohydrate utilisation. The esterase was shown to be active against various HCA-containing substrates and was biochemically characterised in terms of substrate preference, and pH and temperature optima of the enzyme. This novel hydroxycinnamic acid esterase is presumed to be responsible for the release of HCAs from plant-based dietary sources, a process that may have benefits for the gut environment and thus host health.

show abstract

“…Bifidobacteria are common probiotics and strongly sequester iron. Certain high iron-binding strains of bifidobacteria inhibit the growth of iron-dependent pathogens, including Salmonella typhimurium ( S. typhi ) and Escherichia coli O157:H45 [ 8 ]. Our research group conducted a controlled feeding study in older U.S. breastfed infants and found a greater decrease of the genus Bifidobacterium over time in infants consuming iron-fortified cereal from 5–9 months of age [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

Iron in Micronutrient Powder Promotes an Unfavorable Gut Microbiota in Kenyan Infants

et al. 2017

View full text Add to dashboard Cite

Iron supplementation may have adverse health effects in infants, probably through manipulation of the gut microbiome. Previous research in low-resource settings have focused primarily on anemic infants. This was a double blind, randomized, controlled trial of home fortification comparing multiple micronutrient powder (MNP) with and without iron. Six-month-old, non- or mildly anemic, predominantly-breastfed Kenyan infants in a rural malaria-endemic area were randomized to consume: (1) MNP containing 12.5 mg iron (MNP+Fe, n = 13); (2) MNP containing no iron (MNP−Fe, n = 13); or (3) Placebo (CONTROL, n = 7), from 6–9 months of age. Fecal microbiota were profiled by high-throughput bacterial 16S rRNA gene sequencing. Markers of inflammation in serum and stool samples were also measured. At baseline, the most abundant phylum was Proteobacteria (37.6% of rRNA sequences). The proteobacterial genus Escherichia was the most abundant genus across all phyla (30.1% of sequences). At the end of the intervention, the relative abundance of Escherichia significantly decreased in MNP−Fe (−16.05 ± 6.9%, p = 0.05) and CONTROL (−19.75 ± 4.5%, p = 0.01), but not in the MNP+Fe group (−6.23 ± 9%, p = 0.41). The second most abundant genus at baseline was Bifidobacterium (17.3%), the relative abundance of which significantly decreased in MNP+Fe (−6.38 ± 2.5%, p = 0.02) and CONTROL (−8.05 ± 1.46%, p = 0.01), but not in MNP-Fe (−4.27 ± 5%, p = 0.4445). Clostridium increased in MNP-Fe only (1.9 ± 0.5%, p = 0.02). No significant differences were observed in inflammation markers, except for IL-8, which decreased in CONTROL. MNP fortification over three months in non- or mildly anemic Kenyan infants can potentially alter the gut microbiome. Consistent with previous research, addition of iron to the MNP may adversely affect the colonization of potential beneficial microbes and attenuate the decrease of potential pathogens.

show abstract

High Iron-Sequestrating Bifidobacteria Inhibit Enteropathogen Growth and Adhesion to Intestinal Epithelial Cells In vitro

Cited by 48 publications

References 68 publications

Co-occurrence of Campylobacter Species in Children From Eastern Ethiopia, and Their Association With Environmental Enteric Dysfunction, Diarrhea, and Host Microbiome

Co-occurrence of Campylobacter Species in Children From Eastern Ethiopia, and Their Association With Environmental Enteric Dysfunction, Diarrhea, and Host Microbiome

Characterisation of a Hydroxycinnamic Acid Esterase From the Bifidobacterium longum subsp. longum Taxon

Iron in Micronutrient Powder Promotes an Unfavorable Gut Microbiota in Kenyan Infants

Contact Info

Product

Resources

About