High-level expression of HDAC10 is associated with PD-L1 expression and poor prognosis in patients with non-small cell lung cancer receiving pulmonectomy

Liu, X.

doi:10.1093/annonc/mdz438.012

Cited by 1 publication

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This residue contains an N-terminal active deacetylase domain and a C-terminal leucine-rich domain (LRD) that is catalytically inactive (10). Current studies have shown that HDAC10 can participate in the regulation of the occurrence and development of various tumors (11)(12)(13). Nevertheless, the biological function of HDAC10 in the carcinogenesis, progression, and prognosis of ccRCC remains poorly understood.…”

Section: Introductionmentioning

confidence: 99%

Identification of HDAC10 as a candidate oncogene in clear cell renal carcinoma that facilitates tumor proliferation and metastasis.

Yang,

Wei,

Chen

et al. 2024

Preprint

View full text Add to dashboard Cite

Background Clear cell renal carcinoma (ccRCC) seriously threatens people’s health worldwide. Numerous studies have revealed that histone deacetylases (HDACs) affect the cell proliferation, apoptosis and cell differentiation. Nevertheless, the biological function of histone deacetylation modification related genes in the carcinogenesis, progression, and prognosis of ccRCC remains poorly understood. Method Bulk transcriptomic data and clinical information of ccRCC patients were obtained from the TCGA database and the Chinese PLA General Hospital. A total of 36 histone deacetylation genes were included in our study. Univariate cox regression analysis, least absolute shrinkage and selection operator (LASSO) regression, random forest (RF) analysis, and protein-protein interaction (PPI) network analysis were employed to identify key genes affecting the prognosis of ccRCC patients. The ‘oncoPredict’ algorithm was utilized for drug-sensitive analysis. GSEA and KEGG enrichment analysis was used to explore the potential biological function. The ssGSEA algorithm was used for tumor immune microenvironment analysis. The expression levels of HDAC10 were validated by RT-PCR and immunohistochemistry (IHC). EdU assay, CCK-8 assay, cell transwell migration and invasion assay and colony formation assay were performed to detect the proliferation and invasion ability of ccRCC cells. A nomogram incorporating HDAC10 and clinicopathological characteristics was established to predict the prognosis of ccRCC patients. Result Two machine learning algorithms and PPI analysis identified four histone deacetylation genes that have a significant association with the prognosis of ccRCC patients, with HDAC10 being the key gene among them. HDAC10 is highly expressed in ccRCC and its high expression is associated with poor prognosis. Pathway enrichment and the experiments of 5-ethynyl-2′-deoxyuridine (EdU) staining, CCK-8 assay, cell transwell migration and invasion assay and colony formation assay detected that HDAC10 may mediated the proliferation and the metastasis of ccRCC. HDAC10 may also be involved in reshaping the tumor microenvironment (TME) of ccRCC. A clinically reliable prognostic predictive model was established by incorporating HDAC10 and other clinicopathological characteristics ( https://nomogramhdac10.shinyapps.io/HDAC10_Nomogram/ ). Conclusion Our study found the increased expression of HDAC10 was closely associated with poor prognosis of ccRCC patients. HDAC10 may have a pro-tumorigenic effect on ccRCC and promote the proliferation and metastasis of ccRCC, which may provide new light on targeted therapy for ccRCC.

show abstract