High-level expression, purification and pro-apoptosis activity of HIV-TAT-survivin (T34A) mutant to cancer cells in vitro

Expression and Functional Characterization of Tumor-Targeted Fusion Protein Composed of NGR Peptide and 15-kDa Actin Fragment

“…Targeted peptides represent a potentially new class of anticancer agents [24][25][26]. Their activity may be optimized for maximum therapeutic effect by fusing targeted peptide.…”

In vitro production and antifungal activity of peptide ABP-dHC-cecropin A

“…Fermentations were performed as described previously (Ma et al, 2006), with minor modifications. Briefly, a single colony of E. coli BL21 (DE3) harboring the pSUMO ABP-dHC-cecropin A vector was inoculated into 10-mL LB medium supplemented with 50 g/mL kanamycin in a 50-mL flask.…”

Effect on apoptosis and cell cycle of recombinant double negative dominant mutation Survivin (T34/117A) in breast cancer cell B-Cap-37

“…The pRSET-B-TATm-Survivin (T34/117A), Thr117 replaced with Ala117 (Thr117!Ala117), was constructed by overlapping PCR on the base of pRSET-B-TATm-Survivin (T34A) constructed by our laboratory [7]. The plasmid was confirmed by Bam HI/Xho I digestion and sequenced in invitrogen (USA).…”

“…Ma et al obtained a recombinant dominant-negative protein survivin (T34A) with HIV-TAT protein transduction domain, which can automatically penetrate mammalian cell membrane and promote cancer cell apoptosis [7]. Although a homodimer by Xray crystallography [8], survivin can function as a monomer in at least protein-protein interactions, as well as mechanisms of subcellular localization [9].…”