High-level Gentamicin Resistance and Detection of aac (6’)Ie-aph (2”)Ia Gene in Enterococci Isolated from Pediatric Hospital in Northwest of Iran

Choukhachian, Mahsa; Nahaei, Mohammad Reza; Rezaee, Mohammad Ahangarzadeh; Sadeghi, Javid

doi:10.5812/archcid.62921

Cited by 3 publications

(1 citation statement)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Infection by Enterococci are challenging therapeutically as the organism harbors innate resistance toward many antimicrobials and may amass resistance toward many other antibacterial drugs through plasmid transfer or transposons. [ 2 ] Enterococci show intrinsic resistance toward number of antibiotics including cephalosporins, clindamycin, aztreonam, nalidixic acid, trimethoprim-sulfamethoxazole, and fluoroquinolones. [ 3 ]…”

Section: Introductionmentioning

confidence: 99%

High-level gentamicin resistance mediated by Aac(6′)-Ie-aph(2”)-Ia gene in Enterococcus species isolated from clinical samples in Northern India

Das

Dudeja

Kohli

et al. 2022

Indian Journal of Pharmacology

View full text Add to dashboard Cite

CONTEXT: Enterococci are known to cause life-threatening infections which are difficult to treat as the organism harbors innate resistance to many antibiotics and can amass resistance toward many others through plasmid-mediated genetic exchange. AIMS: The study evaluates the drug susceptibility profile of various Enterococcus species isolated from various patient specimens submitted for bacteriological analysis and check the incidence of aac(6′) Ie-aph(2”) Ia gene. SETTING AND DESIGN: This in vitro cross-sectional study was executed at bacteriology laboratory of a 470 bedded hospital in New Delhi. MATERIALS AND METHODS: Drug susceptibility testing was carried out on enterococcal isolates. High-level gentamicin-resistant (HLGR) isolates detected by micro broth dilution assay were then subjected to molecular detection of aac(6′) Ie-aph(2”) Ia gene. STATISTICAL ANALYSIS USED: The level of significance was established by Chi-square test. RESULTS: Among the 182 enterococcal stains detected, 76.9% were Enterococcus faecalis and 20.3% were Enterococcus faecium. 12.08% strains were vancomycin resistant. 39% expressed resistance toward high-level gentamicin (HLG) and this finding was significantly higher in E. faecium than E. faecalis. HLGR strains expressed a higher degree of resistance to other drugs in contrast to non-HLGR isolates. In 67 out of 71 HLGR isolates the bifunctional gene was detected. CONCLUSION: Considerable presence of HLG and vancomycin resistance in the clinical isolates is alarming and should be taken seriously. The study shows high dissemination of aac(6′)-Ie-aph(2”)-Ia gene among Enterococci isolated from the region.

show abstract

Section: Introductionmentioning

confidence: 99%

High-level gentamicin resistance mediated by Aac(6′)-Ie-aph(2”)-Ia gene in Enterococcus species isolated from clinical samples in Northern India

Das

Dudeja

Kohli

et al. 2022

Indian Journal of Pharmacology

View full text Add to dashboard Cite

show abstract

High-Level Aminoglycoside Resistance in Enterococcus Faecalis and Enterococcus Faecium; as a Serious Threat in Hospitals

Khodabandeh

Mohammadi

Abdolsalehi

et al. 2020

IDDT

View full text Add to dashboard Cite

Aims and Objectives: The present work aimed to evaluate the frequency of aminoglycoside- modifying enzymes encoding genes in the E. faecalis and E. faecium and their antibiotic resistance profile. Methods: A total of 305 different clinical samples were subjected for identification and antibiotic susceptibility test. The high-level aminoglycoside resistance was identified by MIC and Kirby Bauer disc diffusion method. The prevalence of aac (6')-Ie-aph (2'')-Ia, aph (3')-IIIa and ant (4')- Ia genes was determined by multiplex- PCR. In total, 100 enterococci strains were isolated. The prevalence of E. faecalis and E. faecium isolates was 78% and 22%, respectively. Results: All isolates were susceptible to linezolid. So, all E. faecalis were susceptible to vancomycin but, 36.4% of E. faecium were resistant to it. The prevalence of multiple drug resistance strains was 100% and 67.9% of E. faecium and E. faecalis, respectively. High-level-gentamicin and streptomycin resistant rates were as follows; 26.9% and 73.1% of E. faecalis and 77.3% and 90.1% of E. faecium. Conclusion: The results of the current study showed a high frequency of aac (6')-Ie-aph (2'')-Ia genes among enterococcal isolates. A high rate of resistance to antimicrobials in Enterococcus is obviously problematic, and a novel policy is needed to decrease resistance in these microorganisms.

show abstract

Virulence determinants and biofilm formation in clinical isolates of Enterococcus: A cross-sectional study

et al. 2020

View full text Add to dashboard Cite

High-level Gentamicin Resistance and Detection of aac (6’)Ie-aph (2”)Ia Gene in Enterococci Isolated from Pediatric Hospital in Northwest of Iran

Cited by 3 publications

References 25 publications

High-level gentamicin resistance mediated by Aac(6′)-Ie-aph(2”)-Ia gene in Enterococcus species isolated from clinical samples in Northern India

High-level gentamicin resistance mediated by Aac(6′)-Ie-aph(2”)-Ia gene in Enterococcus species isolated from clinical samples in Northern India

High-Level Aminoglycoside Resistance in Enterococcus Faecalis and Enterococcus Faecium; as a Serious Threat in Hospitals

Virulence determinants and biofilm formation in clinical isolates of Enterococcus: A cross-sectional study

Contact Info

Product

Resources

About