High level lipid production by a novel inulinase-producing yeast Pichia guilliermondii Pcla22

Wang, Guangyuan; Chi, Zhe; Song, Bo; Wang, Zhipeng

doi:10.1016/j.biortech.2012.08.024

Cited by 51 publications

(28 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The compositions of the medium for lipid production were 60.0 g/L glucose, 7.0 g/L KH 2 PO 4 , 2.5 g/L Na 2 HPO 4 , 1.5 g/L MgSO 4 ·7H 2 O, 0.15 g/L CaCl 2 , 0.15 g/L FeCl 3 ·6H 2 O, 0.02 g/L ZnSO 4 ·7H 2 O, 0.06 g/L MnSO 4 ·H 2 O, and 0.5 g/L yeast extract (Wang et al 2012). The yeast strain was incubated and harvested according to the methods described (Wang et al 2015).…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Systematic analysis of the lysine acetylome reveals diverse functions of lysine acetylation in the oleaginous yeast Yarrowia lipolytica

et al. 2017

View full text Add to dashboard Cite

Lysine acetylation of proteins, a major post-translational modification, plays a critical regulatory role in almost every aspects in both eukaryotes and prokaryotes. Yarrowia lipolytica, an oleaginous yeast, is considered as a model for bio-oil production due to its ability to accumulate a large amount of lipids. However, the function of lysine acetylation in this organism is elusive. Here, we performed a global acetylproteome analysis of Y. lipolytica ACA-DC 50109. In total, 3163 lysine acetylation sites were identified in 1428 proteins, which account for 22.1% of the total proteins in the cell. Fifteen conserved acetylation motifs were detected. The acetylated proteins participate in a wide variety of biological processes. Notably, a total of 65 enzymes involved in lipid biosynthesis were found to be acetylated. The acetylation sites are distributed in almost every type of conserved domains in the multi-enzymatic complexes of fatty acid synthetases. The provided dataset probably illuminates the crucial role of reversible acetylation in oleaginous microorganisms, and serves as an important resource for exploring the physiological role of lysine acetylation in eukaryotes.Electronic supplementary materialThe online version of this article (doi:10.1186/s13568-017-0393-2) contains supplementary material, which is available to authorized users.

show abstract

Section: Methodsmentioning

confidence: 99%

“…The extracted lipids were weighted and oil content per 100 g of cell dry weight was calculated. The lipid particles in the yeast cells were stained by Nile Red and observed according to the methods described (Wang et al 2012). …”

Section: Methodsmentioning

confidence: 99%

Systematic analysis of the lysine acetylome reveals diverse functions of lysine acetylation in the oleaginous yeast Yarrowia lipolytica

et al. 2017

View full text Add to dashboard Cite

show abstract

“…Therefore, it is very important how to enhance fatty acid biosynthesis in the cells by metabolic engineering. It has been well known that many oleaginous yeasts can synthesize over 60 % triacylglycerols (TAGs) in their cells (Wang et al 2012(Wang et al , b, 2015Li et al 2010). So, we think that it is also very crucial how to efficiently transform fatty acids in the lipid particles into alkanes by the oleaginous yeasts.…”

Section: Perspectivesmentioning

confidence: 96%

Hydrocarbons, the advanced biofuels produced by different organisms, the evidence that alkanes in petroleum can be renewable

Chi

et al. 2015

Appl Microbiol Biotechnol

Self Cite

View full text Add to dashboard Cite

It is generally regarded that the petroleum cannot be renewable. However, in recent years, it has been found that many marine cyanobacteria, some eubacteria, engineered Escherichia coli, some endophytic fungi, engineered yeasts, some marine yeasts, plants, and insects can synthesize hydrocarbons with different carbon lengths. If the organisms, especially some native microorganisms and engineered bacteria and yeasts, can synthesize and secret a large amount of hydrocarbons within a short period, alkanes in the petroleum can be renewable. It has been documented that there are eight pathways for hydrocarbon biosynthesis in different organisms. Unfortunately, most of native microorganisms, engineered E. coli and engineered yeasts, only synthesize a small amount of intracellular and extracellular hydrocarbons. Recently, Aureobasidium pullulans var. melanogenum isolated from a mangrove ecosystem has been found to be able to synthesize and secret over 21.5 g/l long-chain hydrocarbons with a yield of 0.275 g/g glucose and a productivity of 0.193 g/l/h within 5 days. The yeast may have highly potential applications in alkane production.

show abstract

“…Recently, inulin and inulin-containing materials have received increasing attention as the renewable raw materials for bioethanol, lipid, citric acid, single-cell protein, ultrahigh-fructose syrup, fructooligosaccharide, and other production chemicals Li et al 2013;Liu et al 2010;Risso et al 2010;Wang et al 2012). Furthermore, inulin has been recognized as one of the important potential non-grain materials for bioethanol production ).…”

Section: Introductionmentioning

confidence: 99%

Enhanced expression of the codon-optimized exo-inulinase gene from the yeast Meyerozyma guilliermondii in Saccharomyces sp. W0 and bioethanol production from inulin

Liu

Chi

2014

Appl Microbiol Biotechnol

Self Cite

View full text Add to dashboard Cite

In the present study, after the exo-inulinase gene INU1 from Meyerozyma guilliermondii was optimized according to the codon usage bias of Saccharomyces cerevisiae, both the optimized gene INU1Y and the native gene INU1 were ligated into the homologous integration expression vector pMIRSC11 and expressed in Saccharomyces sp. W0. It was determined that the inulinase activity of the recombinant yeast Y13 with the optimized gene INU1Y was 43.84 U/mL, which was obviously higher than that (31.39 U/mL) produced by the recombinant yeast EX3 with the native gene INU1. Moreover, it was indicated that the recombinant yeast Y13 could produce 126.30 mg/mL ethanol from 300.0 g/L inulin while the recombinant yeast EX3 and Saccharomyces sp. W0 produced 122.75 mg/mL and 114.15 mg/mL ethanol, respectively, under the same conditions. In addition, the ethanol productivity of the recombinant yeast Y13 was 2.25 mg/mL/h within 48 h of the fermentation, which was obviously higher than that of the recombinant yeast EX3 (1.97 mg/mL/h) and Saccharomyces sp. W0 (1.77 mg/mL/h) within the same period. The results demonstrated that the recombinant yeast Y13 had higher ethanol production and productivity than the recombinant yeast EX3 and Saccharomyces sp. W0. Therefore, it was concluded that the codon optimization of the exo-inulinase gene from M. guilliermondii effectively enhanced inulinase activity and improved ethanol production from inulin by Saccharomyces sp. W0 carrying the optimized inulinase gene.

show abstract

High level lipid production by a novel inulinase-producing yeast Pichia guilliermondii Pcla22

Cited by 51 publications

References 30 publications

Systematic analysis of the lysine acetylome reveals diverse functions of lysine acetylation in the oleaginous yeast Yarrowia lipolytica

Systematic analysis of the lysine acetylome reveals diverse functions of lysine acetylation in the oleaginous yeast Yarrowia lipolytica

Hydrocarbons, the advanced biofuels produced by different organisms, the evidence that alkanes in petroleum can be renewable

Enhanced expression of the codon-optimized exo-inulinase gene from the yeast Meyerozyma guilliermondii in Saccharomyces sp. W0 and bioethanol production from inulin

Contact Info

Product

Resources

About