High level of FHL2 exacerbates the outcome of non‐small cell lung cancer (NSCLC) patients and the malignant phenotype in NSCLC cells

Abstract

Non-small cell lung cancer (NSCLC) is a malignant tumour with high mortality.FHL2 has been identified as a biomarker of lung cancer. This research explored the effects of FHL2 expression on NSCLC. NSCLC-associated data sets were collected from the assistant for clinical bioinformatics and TCGA databases respectively. The association between FHL2 and clinical characteristics, the prognostic significance of FHL2 and the influences of various variables on NSCLC were determined by Pearson's chi-squared test, the Kaplan-Meier curve and the Cox regression model respectively. FHL2 level was altered by cell transfection and was measured by qRT-PCR. Tumour xenograft formation was completed by inoculating sh-FHL2/pcDNA-FHL2 transfected cells into BALB/c nude mice. Protein expression was assessed by western blot. Cell apoptosis, proliferation and epithelial -mesenchymal transition (EMT) characteristics were evaluated employing TUNEL, BrdU + and microscopic observation respectively. The expression of Ki67 and N-cadherin was assessed by immunohistochemistry. The results showed that FHL2 was highly expressed in NSCLC tissues. Patients with high FHL2 expression experienced lower overall survival probability. FHL2 knockdown promoted apoptosis, but inhibited EMT of A549 and NCI-H460 cells, which was verified by the increased ratios of cleaved caspase 9/caspase 9 and cleaved caspase 3/caspase 3, as well as augmented E-cadherin and reduced N-cadherin. In an in vivo assay FHL2 knockdown decreased tumour volume and weight, repressed EMT, but enhanced apoptosis. FHL2 upregulation showed the opposite effects of FHL2 knockdown. Furthermore, FHL2 upregulation facilitated cell proliferation both in in vitro and in vivo assays. These outcomes indicated that high level of FHL2 facilitated tumorigenesis, as well as the proliferation and EMT of NSCLC cells.

Promotion of non-small cell lung cancer tumor growth by FHL2 via inducing angiogenesis and vascular permeability

“…For instance, FHL2 may suppress the growth and differentiation of colorectal cancer cells ( 26 ) but promote cervical cancer cell tumor progression through the regulation of AKT-mTOR pathway ( 27 ). Recently, FHL2 was identified as a biomarker of LUAD that could promote NSCLC progression and migration ( 14 ). Our study reached similar conclusions.…”

“…Four-and-a-half LIM-domain protein 2 ( FHL2 ) can directly interact with several proteins and is involved in various biological progress including cell adhesion, proliferation, apoptosis, invasion, and differentiation ( 10 ). It is reported that FHL2 can promote the progression of several cancers, such as ovarian cancer ( 11 ), cervical cancer ( 12 ), esophageal squamous-cell carcinoma ( 13 ), and NSCLC progression ( 14 ). A recent study showed that FHL2 upregulation facilitated the proliferation and migration of NSCLC cells and exacerbated the negative outcomes of patients with NSCLC ( 14 ).…”

“…It is reported that FHL2 can promote the progression of several cancers, such as ovarian cancer ( 11 ), cervical cancer ( 12 ), esophageal squamous-cell carcinoma ( 13 ), and NSCLC progression ( 14 ). A recent study showed that FHL2 upregulation facilitated the proliferation and migration of NSCLC cells and exacerbated the negative outcomes of patients with NSCLC ( 14 ). More importantly, FHL2 was identified as a biomarker and an invasion-promoting gene of LUAD progression ( 14 , 15 ).…”

“…A recent study showed that FHL2 upregulation facilitated the proliferation and migration of NSCLC cells and exacerbated the negative outcomes of patients with NSCLC ( 14 ). More importantly, FHL2 was identified as a biomarker and an invasion-promoting gene of LUAD progression ( 14 , 15 ). However, the role of FHL2 in NSCLC angiogenesis and vascular permeability has not been elucidated.…”

Revealing Potential Diagnostic Biomarkers of Idiopathic Pulmonary Fibrosis Based on Machine Learning Analysis

“…As for the four diagnostic biomarkers, previous studies showed that FHL2 has been identi ed as a biomarker of lung cancer and high level of FHL2 exacerbates the outcome of patients with non-small cell lung cancer (NSCLC) and the malignant phenotype in NSCLC cells [40]. Among visinin-like proteins (VILIPs), HPCAL1 belongs to the neuronal calcium sensor (NCS) family, which is responsible for calcium signaling in neurons [41].…”