High level production of adrenic acid in Physcomitrella patens using the algae Pavlova sp. Δ5-elongase gene

Kaewsuwan, Sireewan; Bunyapraphatsara, Nantavan; Cove, David J.; Quatrano, Ralph S.; Chodok, Pichit

doi:10.1016/j.biortech.2009.12.138

Cited by 11 publications

(16 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Engineering another thiolase, Δ5-elongase from alga Pavlova sp. into the moss Physcomitrella patens gave high levels of adrenic acid corresponding to the enzyme’s activity in the alga (Kaewsuwan et al 2010). Taken together, it can be concluded that the ACP-KS interaction is permissive across species, directly following from the phylogenetic relatedness and common ancestry of thiolases.…”

Section: Discussionmentioning

confidence: 99%

Evolution of acyl-ACP thioesterases and β-ketoacyl-ACP synthases revealed by protein–protein interactions

Beld

Blatti

Behnke³

et al. 2013

J Appl Phycol

View full text Add to dashboard Cite

The fatty acid synthase (FAS) is a conserved primary metabolic enzyme complex capable of tolerating cross-species engineering of domains for the development of modified and overproduced fatty acids. In eukaryotes, acyl-acyl carrier protein thioesterases (TEs) off-load mature cargo from the acyl carrier protein (ACP), and plants have developed TEs for short/medium-chain fatty acids. We showed that engineering plant TEs into the green microalga Chlamydomonas reinhardtii does not result in the predicted shift in fatty acid profile. Since fatty acid biosynthesis relies on substrate recognition and protein-protein interactions between the ACP and its partner enzymes, we hypothesized that plant TEs and algal ACP do not functionally interact. Phylogenetic analysis revealed major evolutionary differences between FAS enzymes, including TEs and ketoacyl synthases (KSs), in which the former is present only in some species, whereas the latter is present in all, and has a common ancestor. In line with these results, TEs appeared to be selective towards their ACP partners whereas KSs showed promiscuous behavior across bacterial, plant and algal species. Based on phylogenetic analyses, in silico docking, in vitro mechanistic crosslinking and in vivo algal engineering, we propose that phylogeny can predict effective interactions between ACPs and partner enzymes.

show abstract

Section: Discussionmentioning

confidence: 99%

Evolution of acyl-ACP thioesterases and β-ketoacyl-ACP synthases revealed by protein–protein interactions

Beld

Blatti

Behnke³

et al. 2013

J Appl Phycol

View full text Add to dashboard Cite

show abstract

“…The information from our study is currently being used for the development of a larger scale cultivation process for A. maritima TISTR 1715 as an alternative source for commercial ARA. Physcomitrella patens 14 0.40 3.06 [33]…”

Section: Discussionmentioning

confidence: 99%

“…Our study is the first to report the optimization of culture conditions for biomass and ARA production by A. maritima TISTR 1715 even its productivity was lower than the fungi, especially M. alpina which is a current available source for industrial production. Nevertheless, ARA productivity of A. maritima TISTR 1715 was much higher than in the antarctic bacterium strain 651 [7], several algae [23], and some lower plants [33,45] (Table 7). Moreover, the higher growth rate and easier handling of the bacterium make A. maritima a better choice for investigating it as a cheap versatile source of ARA.…”

Section: Optimization Of Screened Factors and An Analysis Of Their Inmentioning

confidence: 95%

“…Total fatty acids from freeze dried cell samples (15 mg) were transmethylated with 2 ml of 2.5% sulfuric acid in methanol at 85°C for 30 min. Fatty acid methyl esters (FAME) were then extracted into 1 ml of heptane, the organic layer evaporated to dryness with a stream of oxygen free nitrogen gas, and the residue dissolved in 500 ll of heptane before gas chromatography (GC) [33]. GC analysis of FAME was conducted using an HP 6890 Series gas chromatograph equipped with an HP-INNOWax capillary column (0.25 mm 9 30 m 9 0.25 lM), a flame ionization detector, using helium as the carrier gas.…”

Section: Fatty Acid Analysismentioning

confidence: 99%

See 1 more Smart Citation

Optimization of Biomass and Arachidonic Acid Production by Aureispira maritima Using Response Surface Methodology

Saelao

Kanjana‐Opas

Kaewsuwan

2010

J Americ Oil Chem Soc

Self Cite

View full text Add to dashboard Cite

Statistically based experimental designs, based on the Plackett-Burman protocol, were applied to the optimization of biomass and arachidonic acid (ARA) production in Aureispira maritima shake-flask cultures. Tryptone and culture temperature were identified to have a significant effect on biomass production, whereas ARA production was only affected significantly by the pH and agitation rate. These four factors were subsequently optimized using response surface methodology. The validity of the optimum conditions was verified by separate experiments in which biomass and ARA yield were increased 4.02-fold (2.05 g l -1 ) and 3.59-fold (21.50 mg g -1 ), respectively, in 3-day fermentations. Under non-optimized culture conditions the corresponding values were 0.51 g l -1 and 5.99 mg g -1 , respectively. The results suggest that A. maritima might be a potential strain for further large scale investigations to determine whether this bacterium might be suitable for commercial production of ARA. To our knowledge, this is the first report of the statistically optimization of biomass and ARA production from the marine gliding bacterium A. maritima.

show abstract

“…Protonemata tissue (14 day-old, 1 g) was aseptically blended in 250-mL sterile shake-flasks containing 100 mL of optimized liquid BCD medium [27] with a homogenizer (OMNI TH Q , USA) at a speed of 30,000 rpm for 1 min and cultivated in a growth room at 25°C in an orbital shaker (New Brunswick Scientific Innova 2100, Champaign, IL, USA) set at 125 rpm under continuous light provided by fluorescent tubes (9,000 lux). After 14 days of growth, the cultured tissues were used to inoculate the liquid medium at a 4% (w/v) level for all subsequent experiments.…”

Section: Starter Inoculum Preparationmentioning

confidence: 99%

Metabolic Engineering and Oil Supplementation of Physcomitrella patens for Activation of C₂₂ Polyunsaturated Fatty Acid Production

Chodok

Cove

Quatrano

et al. 2011

J Americ Oil Chem Soc

Self Cite

View full text Add to dashboard Cite

Long chain (C C 20) polyunsaturated fatty acids (LC-PUFAs) represent important components of the human diet. Currently, the predominant sources of these fatty acids are marine fish and algal oils, but high production costs and diminishing feedstock, limit their supply and usage. A more regular sustainable source of these compounds is urgently required and therefore research is being conducted to develop a sustainable, land-based production system. This work describes the metabolic engineering of an artificial pathway that activates the production of C 22 -PUFAs, docosatetraenoic acid or adrenic acid (ADA) and n-3 docosapentaenoic acid (DPA) in Physcomitrella patens using a gene from a marine algae Pavlova sp. encoding D 5 -elongase and vegetable oil supplementation. The accumulation of ADA and x-3 DPA were dramatically increased to 24.3 and 11.7 mg L -1 and accounted for 2.3 and 1.1% of total fatty acids, respectively. This is the first report on producing n-3 DPA, DHA precursor, in P. patens. The obtained results prove that this enzyme appears to be more active when fused to a green fluorescence protein reporter gene. These finding reveal that the modification of the fatty acid biosynthetic pathway by genetic manipulation and nutritional supplementation, to produce specific PUFAs in a non-seed lower plant, is a promising technique.

show abstract

High level production of adrenic acid in Physcomitrella patens using the algae Pavlova sp. Δ5-elongase gene

Cited by 11 publications

References 37 publications

Evolution of acyl-ACP thioesterases and β-ketoacyl-ACP synthases revealed by protein–protein interactions

Evolution of acyl-ACP thioesterases and β-ketoacyl-ACP synthases revealed by protein–protein interactions

Optimization of Biomass and Arachidonic Acid Production by Aureispira maritima Using Response Surface Methodology

Metabolic Engineering and Oil Supplementation of Physcomitrella patens for Activation of C₂₂ Polyunsaturated Fatty Acid Production

Contact Info

Product

Resources

About

High level production of adrenic acid in Physcomitrella patens using the algae Pavlova sp. Δ5-elongase gene

Cited by 11 publications

References 37 publications

Evolution of acyl-ACP thioesterases and β-ketoacyl-ACP synthases revealed by protein–protein interactions

Evolution of acyl-ACP thioesterases and β-ketoacyl-ACP synthases revealed by protein–protein interactions

Optimization of Biomass and Arachidonic Acid Production by Aureispira maritima Using Response Surface Methodology

Metabolic Engineering and Oil Supplementation of Physcomitrella patens for Activation of C22 Polyunsaturated Fatty Acid Production

Contact Info

Product

Resources

About

Metabolic Engineering and Oil Supplementation of Physcomitrella patens for Activation of C₂₂ Polyunsaturated Fatty Acid Production