High-Level Production of Indole-3-acetic Acid in the Metabolically Engineered <i>Escherichia coli</i>

Wu, Hongxuan; Yang, Jinhua; Shen, Peijie; Li, Qingchen; Wu, Wei; Jiang, Xianzhang; Qin, Lu‐Ping; Huang, Jianzhong; Cao, Xiao; Qi, Feng

doi:10.1021/acs.jafc.0c08141

Cited by 21 publications

(24 citation statements)

References 30 publications

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“…The low-cost medium developed for IAA production with R. fluvialis DMKU-CP293 may facilitate process optimization for economical IAA production at the industrial scale. Recently, high level (7 g/L) of IAA production by metabolically engineered Escherichia coli has been reported 45 . However, this engineered bacterium required inducer and selective pressure to maintain high IAA production.…”

Section: Discussionmentioning

confidence: 99%

Large scale production of indole-3-acetic acid and evaluation of the inhibitory effect of indole-3-acetic acid on weed growth

Bunsangiam

Thongpae

Limtong

et al. 2021

Sci Rep

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Indole-3-acetic acid (IAA) is the most common plant hormone of the auxin class and regulates various plant growth processes. The present study investigated IAA production by the basidiomycetous yeast Rhodosporidiobolus fluvialis DMKU-CP293 using the one-factor-at-a-time (OFAT) method and response surface methodology (RSM). IAA production was optimized in shake-flask culture using a cost-effective medium containing 4.5% crude glycerol, 2% CSL and 0.55% feed-grade l-tryptophan. The optimized medium resulted in a 3.3-fold improvement in IAA production and a 3.6-fold reduction in cost compared with those obtained with a non-optimized medium. Production was then scaled up to a 15-L bioreactor and to a pilot-scale (100-L) bioreactor based on the constant impeller tip speed (Vtip) strategy. By doing so, IAA was successfully produced at a concentration of 3569.32 mg/L at the pilot scale. To the best of our knowledge, this is the first report of pilot-scale IAA production by microorganisms. In addition, we evaluated the effect of crude IAA on weed growth. The results showed that weed (Cyperus rotundus L.) growth could be inhibited by 50 mg/L of crude IAA. IAA therefore has the potential to be developed as a herbicidal bioproduct to replace the chemical herbicides that have been banned in various countries, including Thailand.

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Section: Discussionmentioning

confidence: 99%

Large scale production of indole-3-acetic acid and evaluation of the inhibitory effect of indole-3-acetic acid on weed growth

Bunsangiam

Thongpae

Limtong

et al. 2021

Sci Rep

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“…Furthermore, it has been reported that improving NADPH availability is an essential strategy to increase the yield of the target compounds. 21,22 Of particular interest in this regard is the introduction of an efficient cofactor regeneration system, such as glucose dehydrogenase (GDH) and formate dehydrogenase (FDH), instead of merely using the internal one. 23,24 GDH and FDH oxidize the co-substrates glucose and formate, respectively, to regenerate NADPH and provide enough NADPH for catalytic systems requiring high reducing power.…”

Section: Introductionmentioning

confidence: 99%

“…NADPH could provide the reducing power that drives the anabolic reactions responsible for C‐Spd biosynthesis. Furthermore, it has been reported that improving NADPH availability is an essential strategy to increase the yield of the target compounds 21,22 . Of particular interest in this regard is the introduction of an efficient cofactor regeneration system, such as glucose dehydrogenase (GDH) and formate dehydrogenase (FDH), instead of merely using the internal one 23,24 .…”

Section: Introductionmentioning

confidence: 99%

Overexpression and biochemical characterization of a carboxyspermidine dehydrogenase from Agrobacterium fabrum str. C58 and its application to carboxyspermidine production

et al. 2022

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BACKGROUND Carboxyspermidine (C‐Spd) is a potentially valuable polyamine carboxylate compound and an excellent building block for spermidine synthesis, which is a critical polyamine with significant implications for human health and longevity. C‐Spd can also be used to prepare multivalent cationic lipids and modify nucleoside probes. Because of these positive effects on human health, C‐Spd is of considerable interest as a food additive and pharmaceutical target. RESULTS A putative gene afcasdh from Agrobacterium fabrum str. C58, encoding carboxyspermidine dehydrogenase with C‐Spd biosynthesis activity, was synthesized and transformed into Escherichia coli BL21 (DE3) for overexpression. The recombinant AfCASDH was purified and fully characterized. The optimum temperature and pH for the recombinant enzyme were 30 °C and 7.5, respectively. The coupled catalytic strategy of AfCASDH and various NADPH regeneration systems were developed to enhance the efficient production of C‐Spd compound. Finally, the maximum titer of C‐Spd production successfully achieved 1.82 mmol L−1 with a yield of 91% by optimizing the catalytic conditions. CONCLUSION A novel AfCASDH from A. fabrum str. C58 was characterized that could catalyze the formation of C‐Spd from putrescine and l‐aspartate‐β‐semialdehyde (L‐Asa). A whole‐cell catalytic strategy coupled with NADPH regeneration was established successfully for C‐Spd biosynthesis for the first time. The coupled system indicated that AfCASDH might provide a feasible method for the industrial production of C‐Spd. © 2021 Society of Chemical Industry.

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“…Exogenous PGRs can mitigate the adverse effects of stress (Bielach et al et al 2015). IAA is produced by many plants, yeast (Bunsangiam et al 2021) and Escherichia coli (Wu et al 2021). Auxins act as an active signaling molecule (Weijers and Wagner, 2016).…”

Section: Introductionmentioning

confidence: 99%

Growth and Macronutrients Status of Mung Bean [Vigna radiata (L.) Wilczek] Grown under Lead (Pb) Stress and Exposed to Foliar Spray of Indole Acetic Acid (IAA)

Saima

Haq

Saleem

et al. 2022

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Background: Growth and nutritional status of plant are affected adversely by heavy metals while improved by plant growth regulators. The experiment was aimed to explore the ameliorative potential of indole acetic acid (IAA) for toxicity of Pb. Methods: Two varieties i.e., M-8 and MN-92 of mung bean were grown in pots arranged under complete randomization. Fifteen days after germination, Pb was added @ 10 and 20 mg/kg soil as solutions of PbNO3. Indole acetic acid @100.0 mM was sprayed twice at 15 and 30 days of age. Stem length, root length, leaf area and nutrient ions were recorded at physiological maturity. Three replicates from each treatment were evaluated. Result: The alleviation role of IAA for stem length was recorded as 21.46 and 26.14% for low and high lead stress. The root length was compensated to 34.78 and 31.40% for the respective low and high lead toxicity. Leaf area was affected as 18.11 and 17.32% by IAA. Similarly, Nitrogen contents were nullified to the extents of 15.34 and 8.13%. Phosphorus contents were changed as 11.62 and 4.65% while potassium contents were as 10.14 and 19.36%. The rectifying potential of IAA for leaf biomasses were as 7.64 and 7.43%.

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High-Level Production of Indole-3-acetic Acid in the Metabolically Engineered Escherichia coli

Cited by 21 publications

References 30 publications

Large scale production of indole-3-acetic acid and evaluation of the inhibitory effect of indole-3-acetic acid on weed growth

Large scale production of indole-3-acetic acid and evaluation of the inhibitory effect of indole-3-acetic acid on weed growth

Overexpression and biochemical characterization of a carboxyspermidine dehydrogenase from Agrobacterium fabrum str. C58 and its application to carboxyspermidine production

Growth and Macronutrients Status of Mung Bean [Vigna radiata (L.) Wilczek] Grown under Lead (Pb) Stress and Exposed to Foliar Spray of Indole Acetic Acid (IAA)

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