High-level production of β-1,3-1,4-glucanase byRhizomucor mieheiunder solid-state fermentation and its potential application in the brewing industry

Abstract

The high production yield and excellent capability of the enzyme may enable it great potential in industries, especially in brewing industry.

Fungal β‐1,3‐1,4‐glucanases: production, proprieties and biotechnological applications

“…The mash viscosity was decreased with an increasing enzyme dose. When enzyme was added at 80 U mL −1 , the highest viscosity reduction was obtained (2.61%) . McCarthy et al .…”

“…By contrast to bacteria, fungi require from 4 days in the case of P. thermophila to 7 days in the case of P. occitanis . Under SSF, fungal β ‐1,3‐1,4‐glucanases production was carried out using wheat bran, rice straw and oatmeal as the sole carbon source and maximum recorded β ‐1,3‐1,4‐glucanase activity was 20 025 U g dry substrate −1 (U gds −1 ) for R. miehei followed by 9023.62 U gds −1 for Trichoderma reseei GXC …”

“…Notbly, when EGL was added, the reduction in the filtration time and viscosity were higher compared to the respective values for Finizym . Tang et al . showed that the filtration time was decreased with the increment of enzyme dose.…”

“…Notbly, when EGL was added, the reduction in the filtration time and viscosity were higher compared to the respective values for Finizym. 17 Tang et al 47 showed that the filtration time was decreased with the increment of enzyme dose. The highest filtration time reduction (24.6%) was obtained when -1,3-1,4-glucanase from Rhizomucor miehei was added at a concentration of 60 U mL −1 .…”

“…When enzyme was added at 80 U mL −1 , the highest viscosity reduction was obtained (2.61%). 47 McCarthy et al 60 used 50 IU of crude enzyme from wild-type and two mutants of T. emersonii in mash trials. The results obtained indicated that enzyme added from all three organisms effected a significant reduction in mash viscosity.…”