High Levels of Dietary Supplement Vitamins A, C and E are Absorbed in the Small Intestine and Protect Nutrient Transport Against Chronic Gamma Irradiation

Roche, Marjolaine; Neti, Prasad V.S.V.; Kemp, Francis W.; Azzam, Edouard I.; Ferraris, Ronaldo P.; Howell, Roger W.

doi:10.1667/rr14043.1

Cited by 10 publications

(8 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Charles River 3 , Taconic 4 , LabDiet 5 ) routinely make the composition of their diets publicly available; however, it is incumbent on researchers to ensure that the content of the feed will not impact the radiation exposure study. For example, many diets include known radiation injury protectants/mitigators, such as soy products (Ohara et al 2001;Hillman et al 2011;Abernathy et al 2017;Landauer et al 2019), vitamin A (Seifter et al 1988;Harapanhalli et al 1994;Roche et al 2015;Changizi et al 2019), vitamin C (Narra et al 1994;Konopacka et al 1998 2003; Erol et al 2004;Singh et al 2013). In addition, certain estrogens (Wang et al 2005;Fucic and Gamulin 2011), which have been shown to influence the degree of damage from radiation exposure, may also be found in rodent chow.…”

Section: Dietmentioning

confidence: 99%

See 1 more Smart Citation

Study logistics that can impact medical countermeasure efficacy testing in mouse models of radiation injury

DiCarlo

Horta

Rios

et al. 2020

International Journal of Radiation Biology

View full text Add to dashboard Cite

Purpose: To address confounding issues that have been noted in planning and conducting studies to identify biomarkers of radiation injury, develop animal models to simulate these injuries, and test potential medical countermeasures to mitigate/treat damage caused by radiation exposure. Methods: The authors completed an intensive literature search to address several key areas that should be considered before embarking on studies to assess efficacy of medical countermeasure approaches in mouse models of radiation injury. These considerations include: (1) study variables; (2) animal selection criteria; (3) animal husbandry; (4) medical management; and (5) radiation attributes. Results: It is important to select mouse strains that are capable of responding to the selected radiation exposure (e.g. genetic predispositions might influence radiation sensitivity and proclivity to certain phenotypes of radiation injury), and that also react in a manner similar to humans. Gender, vendor, age, weight, and even seasonal variations are all important factors to consider. In addition, the housing and husbandry of the animals (i.e. feed, environment, handling, time of day of irradiation and animal restraint), as well as the medical management provided (e.g. use of acidified water, antibiotics, routes of administration of drugs, consideration of animal numbers, and euthanasia criteria) should all be addressed. Finally, the radiation exposure itself should be tightly controlled, by ensuring a full understanding and reporting of the radiation source, dose and dose rate, shielding and geometry of exposure, while also providing accurate dosimetry. It is important to understand how all the above factors contribute to the development of radiation dose response curves for a given animal facility with a well-defined murine model. Conclusions: Many potential confounders that could impact the outcomes of studies to assess efficacy of a medical countermeasure for radiation-induced injuries are addressed, and recommendations are made to assist investigators in carrying out research that is robust, reproducible, and accurate.

show abstract

Section: Dietmentioning

confidence: 99%

“…;Kanter and Akpolat 2008;Satyamitra et al 2011;Mortazavi et al 2015;Roche et al 2015;Zangeneh et al 2015;Rostami et al 2016;Alexander et al 2018;Jafari et al 2018), vitamin D(Langberg et al 2009;Gavrilov et al 2010;Marampon et al 2016) and vitamin E(Ross et al 1983;Shaheen and Hassan 1991;Empey et al 1992;Nair et al 2003; Satyamitra et al…”

mentioning

confidence: 99%

Study logistics that can impact medical countermeasure efficacy testing in mouse models of radiation injury

DiCarlo

Horta

Rios

et al. 2020

International Journal of Radiation Biology

View full text Add to dashboard Cite

show abstract

“…Details regarding the vitamin ACE supplemented diet were provided in earlier studies (Roche et al 2011, Roche et al 2015). Briefly, the vitamin ACE supplemented diet was the purified AIN76A diet supplemented with 400 IU g -1 retinyl acetate (100× control), 28.6 mg g -1 ascorbic acid phosphate (35% active), and 12.5 IU g -1 alpha-tocopherol acetate (250× control) (Research Diets, New Brunswick, NJ).…”

Section: Methodsmentioning

confidence: 99%

“…Mice were killed on day 8 after initiating irradiation (i.e. gavage) following the approach of earlier work (Roche et al 2010, Roche et al 2011, Roche et al 2015), and tissue harvested for assessment of each nutrient uptake and residual polonium levels.…”

Section: Methodsmentioning

confidence: 99%

“…Mixtures of radioprotectors offer a means to both improve DMF values and increase coverage with respect to the types of radiation damage that can be protected against. A combination of vitamins A, C and E reduced bone marrow toxicity caused by radioimmunotherapy with 131 I labeled antibodies (Blumenthal et al 2000) and prevented reductions in intestinal nutrient transport caused by whole-body acute and chronic irradiation (Roche et al 2011, Roche et al 2015). …”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Vitamins A, C, and E May Reduce Intestinal 210Po Levels after Ingestion

Kemp

Portugal

Akudugu³

et al. 2016

Health Physics

Self Cite

View full text Add to dashboard Cite

Damage to the gut mucosa is a probable contributory cause of death from ingested 210Po. Therefore, medical products are needed that can prevent, mitigate, and/or repair gastrointestinal (GI) damage caused by high-LET radiation emitted by 210Po. The present studies investigated the capacity of a diet highly enriched with vitamins A, C, and E (vitamin ACE) to protect against intestinal mucosal damage indicated by functional reductions in nutrient transport caused by orally ingested 210Po. Mice were gavaged with 0 or 18.5 kBq 210Po-citrate and fed a control or vitamin ACE-enriched diet (the latter beginning either 96 h before or immediately after gavage). Mouse intestines significantly retained 210Po on day 8 post-gavage. The concentration of 210Po in intestinal tissues was significantly (p<0.05) lower in all vitamin ACE groups compared to control. There were borderline significant 210Po-induced reductions in intestinal absorption of D-fructose. The combination of vitamins A, C, and E may reduce 210Po incorporation in the intestines when given before, or enhance decorporation when provided after, 210Po gavage.

show abstract

Vitamin E alleviates phoxim-induced toxic effects on intestinal oxidative stress, barrier function, and morphological changes in rats

Sun

Zhang

Song

et al. 2018

Environ Sci Pollut Res

View full text Add to dashboard Cite

Phoxim is an organic phosphorus pesticide that remains easily in the environment, such as human food and animal feed. The objective of this study was to explore the effect of vitamin E on phoxim-induced oxidative stress in the intestinal tissues of Sprague-Dawley (SD) rats. Forty-eight Sprague-Dawley rats were randomly assigned to a control group and three treatment groups: treatment group 1 (phoxim: 20 mg/kg·BW), treatment group 2 (phoxim: 180 mg/kg·BW), and treatment 3 (vitamin E + phoxim: 200 mg/kg·BW + 180 mg/kg·BW). Phoxim was given by gavage administration once a day for 28 days. The results showed that phoxim significantly reduced jejunum villus height in rats (P < 0.05), and decreased the mRNA expression of junction protein genes of rats, including Occlidin and Claudin-4 (P < 0.05). Phoxim reduced GSH content and T-AOC level in the intestinal mucosa (P < 0.05). The mRNA expression levels of oxidative stress-related genes (Nrf2 and GPx2) were decreased. The mRNA expression of SOD was significantly increased. In addition, phoxim increased the level of interleukin-6 (IL-6) in jejunum mucosa and significantly reduced the level of IL-8 in ileum mucosas, while significantly increased TNF-α secretion. The mRNA expression levels of IL-1β, IL-6, and IL-8 were significantly decreased, and mRNA expression of TNF-α was significantly increased (P < 0.05). Phoxim also increased the DNA expression of total cecal bacteria and Escherichia coli, inhibited the DNA expression of Lactobacillus and destroyed the intestinal barrier. Two hundred milligrams per kilogram BW vitamin E reduced the effect of phoxim on intestinal structure, alleviated the oxidative stress in intestinal tissue, and decreased the level of TNF-α. The mRNA expressions of antioxidative stress genes (SOD and GPx2) were significantly increased. The DNA expression level of Lactobacillus was significantly increased. In conclusion, vitamin E helped reduce the toxicity of organophosphate pesticides, such as phoxim on rat intestinal tissue.

show abstract

High Levels of Dietary Supplement Vitamins A, C and E are Absorbed in the Small Intestine and Protect Nutrient Transport Against Chronic Gamma Irradiation

Cited by 10 publications

References 47 publications

Study logistics that can impact medical countermeasure efficacy testing in mouse models of radiation injury

Study logistics that can impact medical countermeasure efficacy testing in mouse models of radiation injury

Vitamins A, C, and E May Reduce Intestinal 210Po Levels after Ingestion

Vitamin E alleviates phoxim-induced toxic effects on intestinal oxidative stress, barrier function, and morphological changes in rats

Contact Info

Product

Resources

About