High Levels of Resistance to Chlorantraniliprole Evolved in Field Populations of <I>Plutella xylostella</I>

Abstract

Chlorantraniliprole is the first commercial insecticide from a new class of chemistry, the anthranilic diamides. Chlorantraniliprole provides an effective alternative insecticide for control of Plutella xylostella (L.) populations resistant to other insecticides. Baseline susceptibility to chlorantraniliprole for P. xylostella was surveyed previously from 16 geographical populations sampled from China during 2008-2009, and the median lethal concentrations (LC50s) varied among populations from 1.8- to 8.9-fold higher than the LC50 of a susceptible strain (Roth). In the present work, 20 field populations of P. xylostella sampled in 2010-2011 from China were tested with laboratory bioassays to determine if resistance to chlorantraniliprole had evolved in the field. The LC50s of the 14 populations from northern China ranged from 1.7- to 5.4-fold compared with the LC50 of Roth, which indicates these populations remain reasonably susceptible to chlorantraniliprole. However, the LC50s of the six populations from southern China (Guangdong Province) were 2.6-, 12-, 18-, 81-, 140-, and 2,000-fold higher than the LC50 of Roth. The results showed that high levels of resistance to chlorantraniliprole had evolved in field populations from Guangdong Province of southern China. Intensive use and misuse of chlorantraniliprole may be responsible for the rapid evolution of high-level resistance in P. xylostella in this region. The implementation of resistance monitoring plans and resistance management strategies is urgently needed in China to preserve susceptibility to chlorantraniliprole in P. xylostella.

First report of Tuta absoluta resistance to diamide insecticides

“…However, after several years of field applications, cases of resistance development to diamides have been reported for some lepidopteran species. Cross resistance between flubendiamide and chlorantraniliprole has been reported for diamondback moth Plutella xylostella and smaller tea tortrix, Adoxophyes honmai (Wang and Wu 2012;Uchiyama and Ozawa 2014). In addition, resistance to chlorantraniliprole has been reported in the rice stem borer Chilo suppressalis, the cutworm Spodoptera litura, and the beet armyworm Spodoptera exigua (Su et al 2012;Che et al 2013;Gao et al 2013).…”

Seasonal proportion change of ryanodine receptor mutation (G4946E) in diamondback moth populations

“…The development of resistance against diamides has been reported for P. xylostella in some countries including Thailand, the Philippines, China, and Japan. [5][6][7][8] The diamide resistance of P. xylostella has been shown to be incompletely or almost completely recessive. 7,9,10) The amino acid mutation (G4946E) in RyR has been shown to be a major factor related to target-insensitive diamide resistance in P. xylostella.…”

Molecular characteristics, mRNA expression, and alternative splicing of a ryanodine receptor gene in the Oriental fruit fly,Bactrocera dorsalis(Hendel)

“…With extensive and repetitive applications of diamide insecticides, a low-level resistance, about 9-fold, was first reported in field populations of P. xylostella in China [28]. Subsequently, high levels of resistance to these chemicals evolved in some field populations of P. xylostella from the Philippines, Thailand and China [29][30][31]. Studies on mechanisms of resistance to diamide insecticides in the P. xylostella have been performed by several labs, and, to some extent, metabolic detoxification was involved in chlorantraniliprole resistance in field populations of P. xylostella [31].…”