High lipids accumulation in Rhodosporidium toruloides by applying single and multiple nutrients limitation in a simple chemically defined medium

González‐García, Yolanda; Rábago-Panduro, L. M.; French, Todd; Camacho-Córdova, David Isidoro; Gutiérrez-González, Porfirio; Córdova, Jesús

doi:10.1007/s13213-017-1282-2

Cited by 30 publications

(22 citation statements)

References 29 publications

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“…toruloides ATCC 204091 and used in this study) only accumulated 25–42% oils in the cell mass from different studies [ and references therein]. However, a recent study reported a 77% oil content with R. glutinis ATCC 204091 through a two‐step cultivation strategy . As shown previously, in this study under the same conditions, R. glutinis ATCC 204091 accumulated less oils than Rh.…”

Section: Resultssupporting

confidence: 63%

“…With Rh. toruloides ATCC 204091, a previous study reported a high microbial oil concentration of 43.2 g/L with a cell mass concentration of 60 g/L and oil content of 77% in a two‐step cultivation process with nitrogen limitation . The higher oil production capacity of Rh.…”

Section: Resultsmentioning

confidence: 91%

“…toruloides RP 15 had much higher oil production capacity on all the carbon sources than the two previously reported strains— Rh. toruloides (also reported as Rhodotorula glutinis ATCC 204091) ATCC 204091 and R. mucilaginosa FRR 2406 . With Rh.…”

Section: Resultsmentioning

confidence: 99%

“…toruloides RP 15. For example, a two‐step cultivation process consisting of a nitrogen‐rich step for the growth of cell mass and a subsequent nitrogen‐limiting step for the accumulation of microbial oils may improve the oil concentration and productivity . Metabolic network analysis has revealed the role of oxygen in the improvement of microbial oil production with the various and mixed carbon sources including glycerol , which can be used to guide process optimization (e.g.…”

Section: Resultsmentioning

confidence: 99%

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Co‐utilization of acidified glycerol pretreated‐sugarcane bagasse for microbial oil production by a novel Rhodosporidium strain

Hassanpour

Cai

Gebbie

et al. 2019

Engineering in Life Sciences

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Acidified glycerol pretreatment is very effective to deconstruct lignocellulosics for producing glucose. Co‐utilization of pretreated biomass and residual glycerol to bioproducts could reduce the costs associated with biomass wash and solvent recovery. In this study, a novel strain Rhodosporidium toruloides RP 15, isolated from sugarcane bagasse, was selected and tested for coconversion of pretreated biomass and residual glycerol to microbial oils. In the screening trails, Rh. toruloides RP 15 demonstrated the highest oil production capacity on glucose, xylose, and glycerol among the 10 strains. At the optimal C:N molar ratio of 140:1, this strain accumulated 56.7, 38.3, and 54.7% microbial oils based on dry cell biomass with 30 g/L glucose, xylose, and glycerol, respectively. Furthermore, sugarcane bagasse medium containing 32.6 g/L glucose from glycerol‐pretreated bagasse and 23.4 g/L glycerol from pretreatment hydrolysate were used to produce microbial oils by Rh. toruloides RP 15. Under the preliminary conditions without pH control, this strain produced 7.7 g/L oil with an oil content of 59.8%, which was comparable or better than those achieved with a synthetic medium. In addition, this strain also produced 3.5 mg/L carotenoid as a by‐product. It is expected that microbial oil production can be significantly improved through process optimization.

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Section: Resultssupporting

confidence: 63%

Section: Resultsmentioning

confidence: 91%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

See 2 more Smart Citations

Co‐utilization of acidified glycerol pretreated‐sugarcane bagasse for microbial oil production by a novel Rhodosporidium strain

Hassanpour

Cai

Gebbie

et al. 2019

Engineering in Life Sciences

View full text Add to dashboard Cite

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“…Taking advantage of renewable resources for energy production has become a hot topic worldwide given the decreasing fossil fuel supply and growing environmental problems [1,2]. Biodiesel fuel as one of the most important renewable and clean energy alternatives to fossil fuels, has received wide attention [3].…”

Section: Introductionmentioning

confidence: 99%

Improvement of lipid production in oleaginous yeast Rhodosporidium toruloides by ultraviolet mutagenesis

Guo

Cheng

Chen

et al. 2019

Engineering in Life Sciences

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The oleaginous yeast Rhodosporidium toruloides AS 2.1389 is viewed as desirable industrial microorganisms that can accumulate a high content of lipids for biodiesel production. In this study, we attempted to improve lipid accumulation in the yeast Rhodosporidium toruloides by UV irradiation mutagenesis and selection based on lithium chloride tolerance or ethanol–H2O2 tolerance. The biomass concentration, lipid yield and glucose consumption of mutant R. toruloides were determined. The transcription levels of lipid accumulation‐related genes in the wild‐type and mutant strains were also determined. The lithium chloride‐tolerant strain R‐ZL2 and the ethanol–H2O2‐resistant strain R‐ZY13 were generated by UV mutagenesis. The two mutant strains showed greater lipid productivity and lipid yield compared to the wild type. Transcriptional analysis revealed that IDP1, GPD1 and GND were expressed at significantly higher levels in the two high‐lipid‐producing mutants. In conclusion, lipid productivity and lipid yield in R. toruloides were successfully improved via UV mutagenesis and selection. We also identified some lipid accumulation‐related genes for improving lipid productivity through genetic engineering.

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Attenuating the triacylglycerol catabolism enhanced lipid production of Rhodotorula strain U13N3

Song

Meng

et al. 2023

Appl Microbiol Biotechnol

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High lipids accumulation in Rhodosporidium toruloides by applying single and multiple nutrients limitation in a simple chemically defined medium

Cited by 30 publications

References 29 publications

Co‐utilization of acidified glycerol pretreated‐sugarcane bagasse for microbial oil production by a novel Rhodosporidium strain

Co‐utilization of acidified glycerol pretreated‐sugarcane bagasse for microbial oil production by a novel Rhodosporidium strain

Improvement of lipid production in oleaginous yeast Rhodosporidium toruloides by ultraviolet mutagenesis

Attenuating the triacylglycerol catabolism enhanced lipid production of Rhodotorula strain U13N3

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