High maternal milk intake in the postnatal life reduces the incidence of breast cancer during adulthood in rats

Santiano, Flavia; Zyla, Leila; Verde-Arboccó, F Campo; Sasso, Corina Verónica; Bruna, Flavia; Pistone-Creydt, Virginia; López-Fontana, Constanza; Carón, Rubén W.

doi:10.1017/s2040174418001071

Cited by 6 publications

(5 citation statements)

References 45 publications

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“…These results agree with those previously published [13]. This difference in weight gain is given exclusively by the consumption of breast milk.…”

Section: Body Weightsupporting

confidence: 93%

“…In a preliminary study we demonstrated that animals maintaining more access to maternal milk during postnatal life showed a lower incidence of mammary cancer in the adulthood [13]. This work allowed us to identify the effect over time of variations in postnatal maternal milk consumption, with a controlled animal model.…”

Section: Discussionmentioning

confidence: 91%

“…In a preliminary study, we established an in vivo model of differential milk consumption to investigate the specific role of lactation in the risk of developing mammary carcinoma. We demonstrated that animals maintaining more access to maternal milk during postnatal life generate a lower incidence of mammary cancer in the adulthood [13].…”

Section: Introductionmentioning

confidence: 84%

“…All the animals were decapitated between 10 am and 12 noon on the day of diestrus when the tumors reached a volume 1000 mm 3 or at the end of the experiment on day 250 if they did not develop any mammary tumor [13,31]. When more than one tumor developed in a single rat, the volume limit of 1000 mm3 was considered for the first tumor reaching that value.…”

Section: Sample Collectionmentioning

confidence: 99%

“…(n = 28) born on the same day were distributed at random in litters of different sizes: three (L3) or eight (L8) pups per dam, to induce a differential consumption of maternal milk. The size of the litters was chosen in accordance with previous studies [13,26,27]. Body weight of pups was monitored every 3 days.…”

Section: Introductionmentioning

confidence: 99%

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High Intake of Maternal Milk Prevents the Development of Mammary Cancer in Pups Maintaining Elevated Ingestion of Saturated Fat

Santiano

Sasso

Zyla

et al. 2019

BJSTR

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Environmental factors in early life have a strong implication on the development of diseases in adult life. Nutritional changes during perinatal life can modify the susceptibility to develop breast carcinoma. In this report, we studied the influence of a diet high in saturated fat in the development of breast cancer, in rats that maintained a differential milk intake during their lactation period. We also analysed the possible mechanisms involved in tumor development. We compared mammary carcinogenesis in Sprague-Dawley adult rats, grown-up in litters of 3 (L3) or 8 (L8) pups per mother during lactation to induce a differential consumption of maternal milk. After weaning all pups were fed with a diet high in saturated fat "HF" (40% of energy from lipids) until adulthood. At 55 days of age, the animals were treated with a single dose of dimethylbenzanthracene to study tumor latency, incidence and progression. Histological and immunohistochemical studies were performed. We observed that animals that maintained high milk intake (L3) had lower mammary cancer incidence than animals that maintained lower milk consumption (L8; P <0.05) when exposed to a HF diet. Tumor latency and rate of tumor growth did not show variations between the groups. However, the mitotic index (P< 0.05) and the expression of CD1 were significantly lower, in tumors of L3 respect to L8 (P <0.01). Animals with greater consumption of breast milk develop tumors that proliferate less and tend to have a more pronounced apoptotic process, although when maintaining a high consumption of saturated fat. These results may explain, in part, the lower incidence observed in this group of animals. Our work reflects the importance of lactation during postnatal life in the prevention of breast cancer, despite other nutritional factors that may act as promoters of tumor development throughout life, such as the consumption of saturated fat.

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“…These results agree with those previously published [13]. This difference in weight gain is given exclusively by the consumption of breast milk.…”

Section: Body Weightsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 91%

Section: Introductionmentioning

confidence: 84%

Section: Sample Collectionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%