High Mg/Ca Molar Ratios Promote Protodolomite Precipitation Induced by the Extreme Halophilic Bacterium Vibrio harveyi QPL2

Han, Zuozhen; Qi, Peilin; Zhao, Yanyang; Guo, Na; Yan, Huaxiao; Tucker, Maurice E.; Li, Dan; Wang, Jiajia; Zhao, Hui

doi:10.3389/fmicb.2022.821968

Cited by 21 publications

(15 citation statements)

References 80 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Thus, the halophilic V. harveyi seems to have played a role in the production of calcite and vaterite in our reaction media. V. harveyi is a well-known hyperhalophilic bacteria (Wang et al, 2022;Han et al, 2022) whose ability to precipitate calcite was reported under extreme halophilic conditions. According to our results, this bacterium seems to be able inducing MICP also in non-halophilic media.…”

Section: Resultsmentioning

confidence: 99%

Co-Precipitation of CD, Cr, Pb, Zn, and Carbonates Using Vibrio harveyi Strain Isolated From Mediterranean Sea Sediment

Jarwar¹,

Buey²,

Sanz-Montero³

et al. 2023

Preprint

View full text Add to dashboard Cite

Heavy metal contamination is listed among the most alarming threats to the environment and human health. The detrimental effects of heavy metals in the natural environment span from a reduction of biodiversity to toxic effects on marine life - through microplastic born heavy metals -, to impairment of microbial activity in the soil, and to detrimental effects on animal reproduction. A host of different chemical and biological technologies have been proposed to alleviate environmental contamination by heavy metals. Relatively less attention has been paid to the microbial precipitation of heavy metals, as a side mechanism of the most general process of microbially induced calcite precipitation (MICP). This process is currently receiving a great deal of interest from both a theoretical and practical standpoint, because of its possible practical applications in concrete healing and soil consolidation, and its importance in the more general framework of microbial induced mineral precipitation. In this study, we analyse the ability of the marine bacteria Vibrio harveyi in co-precipitating CaCO3 minerals, together with Cd, Cr, Pb, and Zn added in form of nitrates, from solutions containing CaCl2. The precipitated carbonatic minerals were a function of the different heavy metals present in the solution. The process of co-precipitation appears to be rather effective and fast, as the concentrations of the 4 heavy metals were reduced in 2 days by 97.2%, on average, in the solutions.

show abstract

Section: Resultsmentioning

confidence: 99%

Co-Precipitation of CD, Cr, Pb, Zn, and Carbonates Using Vibrio harveyi Strain Isolated From Mediterranean Sea Sediment

Jarwar¹,

Buey²,

Sanz-Montero³

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“… 25 , 31 The P 2p peak can be ascribed to P–O at 132.8–133.2 eV. 32 The peak of S 2p can be ascribed to S–S at 168.4 and 164.4 eV. Through the XPS analysis, several elements on the surface of minerals constitute different organic functional groups; this is useful to further prove the important influence of organic molecules in the process of mineralization.…”

Section: Resultsmentioning

confidence: 99%

“…The first peak 531.0 eV of O 1s may be attributed to CO 3 , another peak associated with C–O (532.2–532.8 eV). N 1s appeared at a binding energy of 399.5 and 399.7 eV (C–NH 2 ) and is attributable to nonprotonated nitrogen from amines and amides. , The P 2p peak can be ascribed to P–O at 132.8–133.2 eV . The peak of S 2p can be ascribed to S–S at 168.4 and 164.4 eV.…”

Section: Resultsmentioning

confidence: 99%

Mineralogy of Bioprecipitate Evolution over Induction Times Mediated by Halophilic Bacteria under Various Mg/Ca Molar Ratios

Han

Zhao

et al. 2022

ACS Omega

Self Cite

View full text Add to dashboard Cite

In microbial mineralization experiments, the induction time of mineral precipitation is ambiguous, and this may lead to difficulties in reproducing and confirming the test results. To explore the link between induction time and microbially mediated carbonate precipitation, we report here the mineralogy and morphology of carbonate precipitates induced by the halophilic Halomonas utahensis WMS2 bacterium in media with various Mg/Ca molar ratios over a range of induction times. The results show that the biominerals are formed in an alkaline environment affected by ammonia secreted by H. utahensis WMS2 bacteria. The content of dissolved inorganic carbon increased as a result of carbonic anhydrase catalyzing the hydration of carbon dioxide to release bicarbonate and carbonate ions. The X-ray diffraction (XRD) results show that the phase of mineral precipitated gradually changes from an unstable Mg-rich calcite to metastable monohydrocalcite and then to stable hydromagnesite with an increase in the Mg 2+ ion concentration and induction time. The scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDS), and Fourier transform infrared spectroscopy (FTIR) results show that minerals mostly change from single particles/crystallites to aggregations under the action of the microorganisms at different Mg 2+ ion concentrations and induction times. Our experiments demonstrate that the carbonate minerals produced in the presence of microbes change significantly with the induction time, in addition to the influence of the hydrochemical factors; this indicates that the induction time is significant in determining the mineralogy of biominerals.

show abstract

“…The “dolomite enigma” has been a mystery for two centuries at least; compared to the common occurrence in pre-Holocene strata, dolomite is relatively rare in modern marine sediments ( de Dolomieu, 1791 ; Gregg et al, 2015 ; Qiu et al, 2017 ; Zheng et al, 2021 ). After various experiments over many decades, a most popular way to synthesize dolomite in laboratory experiments under earth surface conditions has been to utilize microbes ( Sánchez-Román et al, 2009 , 2011 ; Qiu et al, 2019 ; Liu et al, 2020 ; Han et al, 2022 ). The replacement of Ca 2+ ions by Mg 2+ ions in carbonate minerals has been regarded as the major reason for the formation of dolomite, but this has rarely been reported from laboratory experiments at Earth surface conditions ( Kaczmarek and Thornton, 2017 ; Zheng et al, 2021 ).…”

Section: Discussionmentioning

confidence: 99%

The incorporation of Mg2+ ions into aragonite during biomineralization: Implications for the dolomitization of aragonite

et al. 2023

Self Cite

View full text Add to dashboard Cite

Bacteria can facilitate the increase of Mg2+ content in biotic aragonite, but the molecular mechanisms of the incorporation of Mg2+ ion into aragonite facilitated by bacteria are still unclear and the dolomitization of aragonite grains is rarely reported. In our laboratory experiments, the content of Mg2+ ions in biotic aragonite is higher than that in inorganically-precipitated aragonite and we hypothesize that the higher Mg content may enhance the subsequent dolomitization of aragonite. In this study, biotic aragonite was induced by Bacillus licheniformis Y1 at different Mg/Ca molar ratios. XRD data show that only aragonite was precipitated in the media with Mg/Ca molar ratios at 6, 9, and 12 after culturing for 25 days. The EDS and atomic absorption results show that the content of Mg2+ ions in biotic aragonite increased with rising Mg/Ca molar ratios. In addition, our analyses show that the EPS from the bacteria and the organics extracted from the interior of the biotic aragonite contain the same biomolecules, including Ala, Gly, Glu and hexadecanoic acid. The content of Mg2+ ions in the aragonite precipitates mediated by biomolecules is significantly higher than that in inorganically-precipitated aragonite. Additionally, compared with Ala and Gly, the increase of the Mg2+ ions content in aragonite promoted by Glu and hexadecanoic acid is more significant. The DFT (density functional theory) calculations reveal that the energy needed for Mg2+ ion incorporation into aragonite mediated by Glu, hexadecanoic acid, Gly and Ala increased gradually, but was lower than that without acidic biomolecules. The experiments also show that the Mg2+ ion content in the aragonite significantly increased with the increasing concentration of biomolecules. In a medium with high Mg2+ concentration and with bacteria, after 2 months, micron-sized dolomite rhombs were precipitated on the surfaces of the aragonite particles. This study may provide new insights into the important role played by biomolecules in the incorporation of the Mg2+ ions into aragonite. Moreover, these experiments may contribute towards our understanding of the dolomitization of aragonite in the presence of bacteria.

show abstract

High Mg/Ca Molar Ratios Promote Protodolomite Precipitation Induced by the Extreme Halophilic Bacterium Vibrio harveyi QPL2

Cited by 21 publications

References 80 publications

Co-Precipitation of CD, Cr, Pb, Zn, and Carbonates Using Vibrio harveyi Strain Isolated From Mediterranean Sea Sediment

Co-Precipitation of CD, Cr, Pb, Zn, and Carbonates Using Vibrio harveyi Strain Isolated From Mediterranean Sea Sediment

Mineralogy of Bioprecipitate Evolution over Induction Times Mediated by Halophilic Bacteria under Various Mg/Ca Molar Ratios

The incorporation of Mg2+ ions into aragonite during biomineralization: Implications for the dolomitization of aragonite

Contact Info

Product

Resources

About