High mobilization of CD133+/CD34+ cells expressing HIF-1α and SDF-1α in septic abdominal surgical patients

Cotoia, Antonella; Cela, Olga; Palumbo, Gaetano; Altamura, Sabrina; Marchese, Flavia; Mangialetto, Nicoletta; Bella, Daniela La; Lizzi, Vincenzo; Capitanio, Nazzareno; Cinnella, Gilda

doi:10.1186/s12871-020-01068-w

Cited by 7 publications

(4 citation statements)

References 35 publications

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“…Source control, control of on-going contaminations and restoration of anatomic-physiologic derangement, is the key to clinical management and success, though often difficult to achieve [23]. Numerous blood markers available in routine care aim to identify patients at risk of sepsis and guide timely clinical interventions [7,8,24,25].…”

Section: Discussionmentioning

confidence: 99%

Kinetics of Different Blood Biomarkers during Polymyxin-B Extracorporeal Hemoperfusion in Abdominal Sepsis

Cotoia,

Parisano,

Mariotti

et al. 2024

Blood Purif

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Introduction Comparison of the marker kinetics procalcitonin, presepsin and endotoxin during extracorporeal hemoperfusion with polymyxin B adsorbing cartridge (PMX-HA) have never been described in abdominal sepsis. We aim to compare the trend of three biomarkers in septic post-surgical abdominal patients in Intensive care Unit (ICU) treated with PMX-HA and their prognostic value. Methods Ninety abdominal postsurgical patients were enrolled into different groups according to the evidence of postoperative sepsis or not. Non-septic patients admitted in the surgical ward were included in C group (control group). ICU septic shock patients with endotoxin levels <0.6 EAA receiving conventional therapy were addressed in S group and those with endotoxin levels ≥0.6 EAA receiving treatment with PMX-HA, besides conventional therapy, were included in SPB group. Presepsin, procalcitonin, endotoxin and other clinical data were recorded at 24h (T0), 72h (T1) and 7 days (T2) after surgery. Clinical follow-up was performed on day 30. Results SPB group showed reduced levels of the three biomarkers on T2 vs T0 (P<0.001); presepsin, procalcitonin and endotoxin levels decreased respectively of 25%, 11% and 2% on T1 vs T0, and of 40%, 41%, 26% on T2 vs T0. All patients in C group, 73% of patients in SPB group vs 37% of patients in S group survived at follow-up. Moreover, procalcitonin had the highest predictive value for mortality at 30 days, followed by presepsin. Conclusion The present study showed the reliability of presepsin in monitoring PMX-HA treatment in septic shock patients. Procalcitonin showed better predicting power for the mortality risk.

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Section: Discussionmentioning

confidence: 99%

Kinetics of Different Blood Biomarkers during Polymyxin-B Extracorporeal Hemoperfusion in Abdominal Sepsis

Cotoia,

Parisano,

Mariotti

et al. 2024

Blood Purif

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“…On T-helper lymphocytes, CXCR4 under-expression seems to be a central phenomenon in the idiopathic CD4 lymphopenia [ 29 ], but the infectious profiles are quite different. An alternative explanation could be a chronic elevation of the CXCL12 plasma level, described in severe infection [ 30 , 31 ] and recently in HHT [ 32 ]. Such an elevation is known to lead to continuous CXCR4 and CD26 internalization as a desensitization process [ 9 , 33 ].…”

Section: Discussionmentioning

confidence: 99%

Altered expressions of CXCR4 and CD26 on T-helper lymphocytes in hereditary hemorrhagic telangiectasia

Guilhem

Portalès

Dupuis‐Girod

et al. 2021

Orphanet J Rare Dis

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Background Hereditary hemorrhagic telangiectasia (HHT) is a rare genetic disease characterized by a deregulated neo-angiogenesis. Besides a mainly vascular phenotype (muco-cutaneous telangiectases, arteriovenous malformations), a specific risk of infection is suggested by case series of severe and atypical infections as well as by reports of decreased T and natural killer (NK) lymphocyte counts. As some evidence supports a dysregulation of the CXCR4/CXCL12 chemotactic axis of HHT endothelial cells, we hypothesized that a similar phenomenon could occur on lymphocytes. Methods Eighteen HHT patients with history of severe infection (HSI) were matched in age and sex with 18 HHT without HSI and 18 healthy control subjects (HC). We assessed the cell count and the surface expression of CXCR4 and CD26 (CXCL12 inactivating peptidase) of circulating T-helper and T-cytotoxic lymphocytes (including naive, memory and activated subsets) and NK cells. Results The overall HHT group of 36 patients exhibited a reduction of circulating T-helper lymphocytes compared to HC (median: 517 vs. 1026 cells/mm3, p < 0.0001), correlated with age (r = − 0.46, p = 0.005), requirement of intravenous iron or blood transfusions (median: 291 vs. 627 cells/mm3, p = 0.03) and CXCR4 surface expression (r = 0.353, p = 0.0345). CXCR4 and CD26 membrane expression were both decreased on HHT T-helper lymphocytes (median MFI ratio: 4.49 vs. 5.74 for CXCR4 and 3.21 vs. 4.33 for CD26, p = 0.03 and 0.0018 respectively) with an unchanged CXCR4/CD26 ratio. The HHT group with HSI had a higher CXCR4/CD26 ratio on the total T-lymphocyte population, as well as on the T-helper population and its naive subset (median on naive T-helper cells: 2.34 vs. 1.32, p = 0.0002). Conclusions Our findings support a dysregulation of the CXCL12/CXCR4 chemotaxis of T-helper lymphocytes in HHT patients, potentially linked to their T-helper lymphopenia and susceptibility to infection.

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“…SDF-1 является хемоаттрактантом для клеток, которые имеют на мембране его рецепторы (CXCR4), в частности ЭПК костного мозга экспрессируют эти рецепторы на своей поверхности [15]. В условиях воспаления и гипоксии в месте повреждения гипоксический эндотелий и активированные тромбоциты секретируют SDF-1, экспрессия которого стимулируется и регулируется гипоксия-индуцибельным фактором-1α (HIF-1α) [15,16]. SDF-1 связывается с CXCR4-рецептором гемопоэтических стволовых клеток, что способствует CXCR4-опосредованной мобилизации ЭПК из костного мозга в кровь и их миграции к очагу поражения [15,16].…”

Section: Introductionunclassified

“…В условиях воспаления и гипоксии в месте повреждения гипоксический эндотелий и активированные тромбоциты секретируют SDF-1, экспрессия которого стимулируется и регулируется гипоксия-индуцибельным фактором-1α (HIF-1α) [15,16]. SDF-1 связывается с CXCR4-рецептором гемопоэтических стволовых клеток, что способствует CXCR4-опосредованной мобилизации ЭПК из костного мозга в кровь и их миграции к очагу поражения [15,16]. SDF-1-зависимая мобилизация ЭПК способствует стабилизации HIF-1α в мобилизованных ЭПК.…”

Section: Introductionunclassified

Endothelial progenitor cells in pathogenesis of new coronaviral infection

et al. 2021

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Pulmonary vascular endothelium dysfunction is one of the main pathogenic factors responsible for many clinical manifestations of the severe course of COVID-19. Circulating endothelial progenitor cells (EPCs) are the endogenous regenerative reserve that maintains the integrity of the vascular endothelium and its restoration in case of damage by pathogenic factors. A decrease in the circulating EPCs is regarded as a predictor of morbidity and mortality in conditions associated with development of endothelial dysfunction, including COVID-19. The exact phenotype of progenitor cells capable of differentiating into endothelial cells has not been determined. In most laboratories antigens CD133+, CD34+, VEGFR-2+ (CD 309) or combination of these are used to identify EPCs. The process of EPCs mobilization and migration is controlled by molecular signals from immune cells located in the damage area. Stromal cell factor 1 (SDF-1), produced by the bone marrow and many other tissues, is an important chemoattractant for EPCs which express its receptors. The results of studies carried out in 2020 indicate that SARS-Cov-2 infects both hematopoietic stem cells, transforming into EPCs, and directly circulating EPCs, causing inflammatory and procoagulant reactions that complicate the COVID-19 course. There is no consensus on the mechanism of EPCs infection with coronavirus – directly through the expression of angiotensin-converting enzyme (ACE2) receptor or through an ACE2-independent mechanism. Today there is no effective therapy for COVID-19. The use of the EPCs regenerative potential, and the search for ways to enhance the EPCs mobilization from the depot, and increase their functional activity may become a promising approach to the prevention of severe complications and mortality from COVID-19.

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High mobilization of CD133+/CD34+ cells expressing HIF-1α and SDF-1α in septic abdominal surgical patients

Cited by 7 publications

References 35 publications

Kinetics of Different Blood Biomarkers during Polymyxin-B Extracorporeal Hemoperfusion in Abdominal Sepsis

Kinetics of Different Blood Biomarkers during Polymyxin-B Extracorporeal Hemoperfusion in Abdominal Sepsis

Altered expressions of CXCR4 and CD26 on T-helper lymphocytes in hereditary hemorrhagic telangiectasia

Endothelial progenitor cells in pathogenesis of new coronaviral infection

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