“…The tubes were centrifuged (1000 g for 2 min) in a Biofuge Primo Centrifuge (Heraeus Instruments, Kendro Laboratory Products, D-37520 Osterode, Germany) to clarify them, and an aliquot of the supernatant was removed for analysis of reducing sugars. The remaining digest was filtered to separate the undigested residue from the supernatant (Thondre, Monro, Mishra, & Henry, 2010).…”