2003
DOI: 10.1016/s0006-8993(03)03119-6
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High molecular weight DNA fragments are processed by caspase sensitive or caspase independent pathways in cultures of cerebellar granule neurons

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Cited by 34 publications
(23 citation statements)
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“…Removing serum and lowering potassium concentration from the culture medium has been reported to induce apoptosis of cerebellar granule cells (37)(38)(39). Under these conditions, in accordance with previous studies (37,49,50,56,57), our results showed an increase of DNA fragmentation and caspase-3 activity, which could be prevented in a dose-and time-dependent manner by a peptidic agonist of C5aR (MAP-C5a), which highlights the role of anaphylatoxins on tissue remodeling, as has been hypothesized by Mastello and Lambris (58). Concerning C3a, in which the receptor was also expressed by granule cells, no effect on cell survival could be observed in this model.…”
Section: Discussionsupporting
confidence: 89%
See 1 more Smart Citation
“…Removing serum and lowering potassium concentration from the culture medium has been reported to induce apoptosis of cerebellar granule cells (37)(38)(39). Under these conditions, in accordance with previous studies (37,49,50,56,57), our results showed an increase of DNA fragmentation and caspase-3 activity, which could be prevented in a dose-and time-dependent manner by a peptidic agonist of C5aR (MAP-C5a), which highlights the role of anaphylatoxins on tissue remodeling, as has been hypothesized by Mastello and Lambris (58). Concerning C3a, in which the receptor was also expressed by granule cells, no effect on cell survival could be observed in this model.…”
Section: Discussionsupporting
confidence: 89%
“…7A). As previously reported (48,49), a 6-h incubation of DIV 4 granule cells with S-K5 medium caused a robust activation of caspase-3 (Fig. 7B).…”
Section: Expression Of the C3ar And C5ar Proteins During Differentiatsupporting
confidence: 88%
“…Glutamate produces an insult-dependent continuum of apoptosis and necrosis in cultured cortical neurons, where necrosis predominates after intense insults but mild insults result in apoptotic cell death (28). In our experiments we treated the cells with 100 M glutamate, which is a mild insult and thus initiates apoptosis rather than necrosis (29).…”
Section: Discussionmentioning
confidence: 99%
“…AIF (apoptosis inducing factor), an essential flavoprotein released from mitochondria during apoptotic execution, can cause nuclear chromatin to condense into a ring at the nuclear periphery [2]. Although AIF has been reported to activate nuclease activity capable of generating high molecular weight (HMW) DNA fragments [3,4], its requirement for the generation of those fragments has been disputed [5,6], and the underlying mechanism by which it promotes peripheral chromatin condensation is unknown. If AIF is depleted by RNAi, nuclear disassembly occurs normally, or is even enhanced in cells with a functional CAD/ICAD system [6].…”
Section: Introductionmentioning
confidence: 99%