High‐molecular‐weight Fibronectin Synthesized by Adenoid Cystic Carcinoma Cells of Salivary Gland Origin

Toyoshima, Koh-éi; Kimura, Shinn; Cheng, Jun; Oda, Y.; Mori, Kazuhiro; Saku, Takashi

doi:10.1111/j.1349-7006.1999.tb00749.x

Cited by 19 publications

(21 citation statements)

References 36 publications

(39 reference statements)

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“…The appearance of the high-molecular-weight forms of FN has also been reported in diseases other than dementia [16,17] . The diverse composition of the molecular forms of FN was found to be associated with lung cancer and, to a significantly lesser degree, with lung inflammation [16] .…”

Section: Discussionmentioning

confidence: 99%

“…The diverse composition of the molecular forms of FN was found to be associated with lung cancer and, to a significantly lesser degree, with lung inflammation [16] . According to Toyoshima et al [17] , the 315-kDa form of FN found in the plasma of salivary gland carcinoma patients partly resulted from the expression of alternatively spliced (ED-A, ED-B, and IIICS domains) segments and partly from glycosylation with both Olinked and N-linked oligosaccharide chains. On the other hand, FN has the ability to form cross-linked complexes with some blood plasma components, such as fibrin, fibrinogen [18,19] and fragments derived from FN degradation processes [19] as well as with various ECM components [20] and smaller molecules such as homocysteine [21] , whose concentration was elevated in AD [22] .…”

Section: Discussionmentioning

confidence: 99%

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Molecular Status of Plasma Fibronectin as an Additional Biomarker for Assessment of Alzheimer’s Dementia Risk

Lemańska-Perek

Leszek²,

Krzyżanowska-Gołą³

et al. 2009

Dement Geriatr Cogn Disord

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Background:Aberrant angiogenesis and senescence of the cerebrovascular system could initiate neurovascular events leading to neurovascular disorders. Fibronectin (FN) exerts a strong angiogenic influence on endothelial cells in the central nervous system. Methods: In the present study the expression of the plasma fibronectin molecular forms in an Alzheimer patient group, compared with vascular dementia and age-matched control groups was analyzed by immunoblotting. Results: FN in the plasma of the elderly individuals with and without dementia exists as a mixture of heterogeneous molecules with increasing molecular masses. Apart from the wide ∼240-kDa and ∼220-kDa FN bands, normally present in plasma, the high molecular FN forms having ∼280 kDa and ∼320 kDa appeared in the plasma of the Alzheimer dementia group more frequently and at the higher amounts than in the vascular dementia and age-matched nondemented groups. Conclusions: The plasma FN molecular status seems to be a molecular additional biomarker for assessment of dementia risk.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Molecular Status of Plasma Fibronectin as an Additional Biomarker for Assessment of Alzheimer’s Dementia Risk

Lemańska-Perek

Leszek²,

Krzyżanowska-Gołą³

et al. 2009

Dement Geriatr Cogn Disord

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“…ACC3 cells were kindly provided by Dr Takashi Saku of Niigata University School of Dentistry, Japan and were cultured in RPMI-1640 medium (Life Technologies cat# 10370-021) containing 15% (v/v) fetal calf serum, 1% glutamine, 1% amphotericin B, 1% streptomycin and 1% penicillin and incubated in a humidified 5% CO 2 /95% air atmosphere at 371C. This cell line has been shown to have a gene expression profile similar to that of primary ACC in microarray experiments, 19 has basement membrane synthesis function similar to primary ACC 16,18,20,21 and when grown as a xenograft tumor in immunodeficient mice, have a histologic appearance consistent with a grade III ACC. 16 DNA from confluent ACC3 cells was extracted similarly to the tissue samples, with the addition of phenol/chloroform extraction and ethanol precipitation.…”

Section: Cell Culturementioning

confidence: 99%

Mapping of candidate tumor suppressor genes on chromosome 12 in adenoid cystic carcinoma

Rutherford

Frierson

Moskaluk

2005

Laboratory Investigation

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“…cDNA was synthesized from the RNA with the SuperScript Preamplification System (Gibco BRL). Following the reverse transcription (RT), polymerase chain reaction (PCR) was carried out in an Astec thermal cycler PC-800 (Astec Co., Ltd., Fukuoka) as previously described [30].…”

Section: Reverse-transcriptase Polymerase Chain Reactionmentioning

confidence: 99%

The basement membrane-type heparan sulfate proteoglycan (perlecan) in ameloblastomas: its intercellular localization in stellate reticulum-like foci and biosynthesis by tumor cells in culture

et al. 2001

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The localization and biosynthesis of basement membrane-type heparan sulfate proteoglycan (HSPG), known as perlecan, were studied in ameloblastomas using surgical tissue sections and cells in primary culture to demonstrate the existence of extracellular matrix (ECM) molecules in the intercellular space of epithelial tissue. HSPG was immunolocalized in the intercellular spaces of stellate reticulum-like cells and small vacuolar structures between basal cells in tumor cell nests as well as in myxofibrous stroma. By means of in-situ hybridization, mRNA signals for the HSPG core were intensely demonstrated in the cytoplasm of basal and parabasal cells of parenchyma. Furthermore, the in-vitro biosynthesis of HSPG core protein by ameloblastoma cells was confirmed using immunofluorescence, immunoprecipitation, and reverse-transcriptase polymerase chain reaction (RT-PCR). The results indicated that ameloblastoma cells synthesize HSPG and deposit it in their intercellular space. The intercellular HSPG might act as a carrier for transport of nutrients to tumor cells within ameloblastomatous foci.

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High‐molecular‐weight Fibronectin Synthesized by Adenoid Cystic Carcinoma Cells of Salivary Gland Origin

Cited by 19 publications

References 36 publications

Molecular Status of Plasma Fibronectin as an Additional Biomarker for Assessment of Alzheimer’s Dementia Risk

Molecular Status of Plasma Fibronectin as an Additional Biomarker for Assessment of Alzheimer’s Dementia Risk

Mapping of candidate tumor suppressor genes on chromosome 12 in adenoid cystic carcinoma

The basement membrane-type heparan sulfate proteoglycan (perlecan) in ameloblastomas: its intercellular localization in stellate reticulum-like foci and biosynthesis by tumor cells in culture

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