High Molecular Weight Lectin Isolated from the Mucus of the Giant African Snail<i>Achatina fulica</i>

Ito, Shigeru; Shimizu, Masahiro; Nagatsuka, Maki; Kitajima, Seiji; Honda, Michiyo; Tsuchiya, Takahide; Kanzawa, Nobuyuki

doi:10.1271/bbb.100389

Cited by 33 publications

(31 citation statements)

References 34 publications

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“…Another source of glycan-binding proteins however is the host's mucus. The presence of lectins in mucus of molluscs has been reported and several lectins have been identified in mucus covering the snails and bivalves (Iguchi et al 1985, Pales Espinosa et al 2009, Ito et al 2011. While our experimental dataset supports the suggestion that lectin binding to the surface may trigger the transformation of F. hepatica miracidium into sporocyst, the relevance to in vivo situations still needs knowledge on glycan-binding proteins available in the mucus of the gastropod host G. truncatula.…”

Section: Discussionsupporting

confidence: 70%

“…Lectins are a component of snail hemolymph protective arsenal but they are also present in the mucus secreted by the molluscans (Iguchi et al 1985, Pales Espinosa et al 2009, Ito et al 2011. To check the possibility of lectin binding as a trigger for miracidial transformation, we examined the interference of commercially available lectins with in vitro miracidium-to-sporocyst transformation.…”

Section: Introductionmentioning

confidence: 99%

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Fasciola hepatica miracidia: Lectin binding and stimulation of in vitro miracidium-to-sporocyst transformation

Georgieva

Mizinska

et al. 2012

Acta Parasitologica

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The lectin binding properties of Fasciola hepatica miracidia were studied by a panel of fluorescein-and gold-conjugated lectins (ConA, LCA, WGA, LEA, SBA, HPA and UEA-I). The presence of mannose and/or glucose residues was demonstrated with ConA and LCA as weak diffuse fluorescence of the miracidial surface, which was more intense at the anterior part of the larva. The N-acetylglucosamine-binding lectins WGA and LEA reacted intensely with the whole miracidial surface. No labelling with N-acetylgalactosamine and/or galactose-specific (SBA and HPA) and fucose-specific UEA-I lectins was observed. The possibility that the specific recognition of the miracidial surface carbohydrates by lectins may initiate the process of transformation of the miracidia into sporocysts was examined in vitro in physiological saline for Galba truncatula. Incubation in the presence of ConA and WGA resulted in facilitation of the transformation process. Facilitation was absent in the presence of inhibitor sugars. Incubation in the presence of SBA or UEA-I had no effect. The results suggested a possible impact of carbohydrate-lectin interactions in transformation of miracidia of F. hepatica to sporocysts in vivo.

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Section: Discussionsupporting

confidence: 70%

Section: Introductionmentioning

confidence: 99%

Fasciola hepatica miracidia: Lectin binding and stimulation of in vitro miracidium-to-sporocyst transformation

Georgieva

Mizinska

et al. 2012

Acta Parasitologica

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“…It is similar to lectins (C-type) in the body surface mucus of D. japonica [18]. Also, the majority of invertebrate lectins are C-type lectins [14,17,[40][41][42][43][44], which require calcium for their agglutinating activity. However, it differs from D-galactoside-specific lectins in amphioxus [11], lectins in the shrimp Litopenaeus schmitti [45] and lectins in the sea cucumber [12].…”

Section: Discussionmentioning

confidence: 99%

Purification, characterization and induction of a C-type lectin in the freshwater planarian Dugesia japonica

et al. 2012

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Lectins are important components of the immune defense system of invertebrates. Given their important functions, numerous investigations have been carried out on the characterization and function of lectins in invertebrates. However, lectin studies with the freshwater planarian, an evolutionarily important animal, are rare. In this paper, we demonstrate agglutination of glutaraldehyde treated erythrocytes by a lectin with preference for rabbit erythrocytes. The result of hemagglutinating activity inhibition assays with several carbohydrates showed the most potent inhibitor was maltose. A natural lectin from the crude homogenates of freshwater planarian Dugesia japonica was purified by single step affinity chromatography using amylose-coupled agarose. The purified protein appeared as one band with a molecular mass of 350 kDa in PAGE, and as one band, approximately 56 kDa, in SDS-PAGE. The purified lectin showed dependence on calcium. The activity of the purified lectin was inhibited at temperatures greater than 50°C and showed a pH optimum between 5–8. The purified lectin also has binding activity to the Gram-negative bacteria E. coli, and the Gram-positive bacteria B. subtilis. Furthermore, the purified lectin obtained from injured and bacteria-induced planarians showed increased agglutinating activity against rabbit erythrocytes. These results suggest that the purified lectin may play an important role in the innate immunity of the freshwater planarian.

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“…Hasil analisis proksimat kadar air daging segar keong kowoe adalah 63,79%, setelah mengalami proses pengeringan dengan oven suhu 50-60°C mempunyai kadar air sebesar 40,04%. Kadar air daging segar keong kowoe tidak jauh berbeda dengan kadar air yang ditemukan pada Fasciolaria salmo yaitu 73,075%, sedangkan kadar air pada keong kowoe kering 40,04% (Nurjanah et al 2011 Ito et al (2011) melaporkan bahwa lektin dari Achatina fulica dapat menggumpalkan sel darah merah dan eritrosit dari domba dan kelinci.…”

Section: Komposisi Kimiaunclassified

Characterization of Kowoe snail and its antioxidant activity

2017

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AbstrakKeong mas atau dalam bahasa daerah Sulawesi (Kabupaten Konawe) disebut sebagai "Keong kowoe" banyak dimanfaatkan masyarakat sebagai makanan. Keong ini dipercaya memiliki banyak khasiat dan manfaat, antara lain kandungan antioksidan yang tinggi. Penelitian ini bertujuan menentukan pelarut yang terbaik untuk mengekstrak komponen bioaktif keong mas dan menentukan kandungan antioksidannya. Penelitian ini meliputi analisis proksimat, uji kuantitatif aktivitas antioksidan dengan metode DPPH. Hasil pengukuran morfometrik menunjukkan rendemen daging sebesar 11,86%. Kandungan proksimat daging segar tertinggi pada kadar air sebesar 65,79%, protein 18,14%, abu 10,49%, karbohidrat 4,14% dan lemak 3,44%, sedangkan pada daging kering kadar air sebesar 40,04%, protein 38,06%, karbohidrat 10,66%, abu 8,53% dan lemak 2,70%. Hasil pengamatan didapatkan rendemen hasil ekstraksi senyawa aktif menggunakan pelarut kloroform (non polar) sebesar 1,78%, etil asetat (semi polar) 3,41% dan metanol 6,63% (polar). Rendemen yang tinggi pada pelarut metanol diduga karena keong kowoe mengandung lebih banyak senyawa aktif yang bersifat polar. Hasil uji aktivitas antioksidan ekstrak kasar jenis pelarut metanol (polar) mempumyai nilai IC 50 yaitu 111,28 ppm, sehingga tergolong sedang.Kata kunci: antioksidan, bioaktif, proksimat, rendemen Abstract Gold snail is called "Kowoe" in Celebes Island. They are widely utilized by people as food. This snail also has many properties and benefits. The aims of this study were to determine the best solvent to extract the bioactive components of snails and measure the antioxidant activity. The proximate analysis was caried out, then quantitative test of antioxidant activity by DPPH method was measured. The results of morphometric measurements showed the meat yield 11.86%. The proximate analysis of fresh meat consisted of water content 65.79%, 18.14% protein, 10.49% ash, 4.14% carbohydrates, and fats 3.44%, while the moisture content of dried meat contained 40/ 04%, 38.06% protein, carbohydrates 10.66%, ash 8.53% and 2.70% fat, respectively. The yield of extraction of the active compound using chloroform (non-polar) was 1.78%, ethyl acetate (semi-polar) was 3.41% and 6.63% methanol (polar). The snails kowoe contain more the polar of active compounds. The antioxidant activity of crude extract in methanol solvent (polar) had IC 50 values 111.28 ppm (moderate).

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High Molecular Weight Lectin Isolated from the Mucus of the Giant African SnailAchatina fulica

Cited by 33 publications

References 34 publications

Fasciola hepatica miracidia: Lectin binding and stimulation of in vitro miracidium-to-sporocyst transformation

Fasciola hepatica miracidia: Lectin binding and stimulation of in vitro miracidium-to-sporocyst transformation

Purification, characterization and induction of a C-type lectin in the freshwater planarian Dugesia japonica

Characterization of Kowoe snail and its antioxidant activity

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