High multiplication frequency and genetic stability for commercialization of the three varieties of micropropagated tea plants (Camellia spp.)

Borchetia, Sangeeta; Handique, Pratap Jyoti; Das, Sudripta

doi:10.1016/j.scienta.2008.12.007

Cited by 36 publications

(13 citation statements)

References 11 publications

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“…Similar observations have been reported by Malik et al (1993) in Lathyrus species and Poisetty et al (1997) in Cicer arietinum, where adventitious origin of multiple shoots from epicotyl region was found directly from intact seeds cultured on different cytokinin containing medium (BA and TDZ). Borchetia et al (2009) reported that the plantlets derived from adventitious mode of propagation can be genetically true to type and this mode of plant regeneration is more reliable than callus cultures. Shoots produced in vitro were excised and treated with different concentrations of root inducing auxins namely IBA (Fig.…”

Section: Resultsmentioning

confidence: 99%

In vitro plant regeneration of Albizia lebbeck (L.) from seed explants

Perveen¹,

Anis²,

Aref³

2013

For. syst.

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Objectives: An efficient and reproducible regeneration protocol for rapid multiplication of Albizia lebbeck (L.) was developed by using intact seed explants.Methods: Murashige and Skoog's (MS) medium supplemented with different hormones (BA, Kn, GA3 and TDZ) was used for the induction of multiple shoots from the seed explants. Ex-vitro rooting was performed by using pulse treatment method in auxins (IBA and NAA) and the complete plantlets were transferred to the field.Results: High frequency direct shoot induction was found in aseptic seed cultures of A. lebbeck on Murashige and Skoog medium supplemented with 5.0 µM TDZ (Thiadiazuron). Seeds were germinated after 7 days of culture and induced maximum 8 shoots from the region adjacent to the apex of the primary shoot of the seedling upto 25 days of incubation. Proliferating shoot cultures with increased shoot length was established by sub-culture of excised sprouting epicotyls on MS medium supplied with reduced concentrations of TDZ. Maximum shoot regeneration frequency (76 %) with highest number of shoots (21) and shoot length (5.1 cm) per sprouting epicotyl was observed in the MS medium supplemented with 0.5 µM TDZ after 8 weeks of culture. Different concentrations of Indole-3-butyric acid (IBA) and α-naphthalene acetic acid (NAA) were tested to determine the optimal conditions for ex-vitro rooting of the microshoots. The best treatment for maximum ex-vitro root induction frequency (81 %) was accomplished with IBA (250 µM) pulse treatment given to the basal end of the microshoots for 30 min followed by their transfer in plastic cups containing soilrite and eventually established in normal garden soil + soilrite (1:1) with 78 % survival rate. In addition, histological study was undertaken to gain a better understanding of the regenerated shoots from the epicotyl region.Conclusion: The findings will be fruitful in getting a time saving and cost effective protocol for the in vitro propagation of Albizia lebbeck.Keywords: Woody legume; TDZ; multiplication; epicotyl segment; shoot stumps.Abbreviations used: GA3 (Gibberellic acid); BA (6-Benzyladenine); Kn (Kinetin); TDZ (Thidiazuron); MS (Murashige and Skoog Medium); IBA (Indole-3-butyric acid); NAA (α-Naphthalene acetic acid).

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Section: Resultsmentioning

confidence: 99%

In vitro plant regeneration of Albizia lebbeck (L.) from seed explants

Perveen¹,

Anis²,

Aref³

2013

For. syst.

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“…The technique exploits the usual ontogenic route of branch development by lateral (axillary meristems). Axillary meristem culture has gained the status of a multibillion-dollar industry throughout the world [17]. Many ornamental and medicinal plant species are propagated commercially by axillary bud proliferation [18].…”

Section: Introductionmentioning

confidence: 99%

Interactive Effects of Growth Regulators, Carbon Sources, pH on Plant Regeneration and Assessment of Genetic Fidelity Using Single Primer Amplification Reaction (SPARS) Techniques in Withania somnifera L.

Fatima

Ahmad

et al. 2015

Appl Biochem Biotechnol

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An improved and methodical in vitro shoot morphogenic approach through axillary bud multiplication was established in a drug yielding plant, Withania somnifera L. Effects of plant growth regulators [6-benzyladenine (BA), kinetin (Kin), 2-isopentenyladenine (2iP), and thidiazuron (TDZ)] either singly or in combination with α-napthalene acetic acid (NAA), indole-3-butyric acid (IBA), and indole-3-acetic acid (IAA) in Murashige and Skoog (MS) medium were tested. The highest regeneration frequency (90 %) with optimum number of shoots (32 ± 0.00)/explant were obtained on MS medium fortified with 2.5 μM 6-benzyladenine (BA) and 0.5 μM NAA and 30 g/l sucrose at pH 5.8. Among the tried TDZ concentrations, 0.5 μM resulted in maximum number of shoots (20.4 ± 0.40)/explant after 4 weeks of exposure. The proliferating shoot cultures established by repeated subculturing of the mother explants on the hormone-free medium produced the highest shoot number (29.4 ± 0.40) with shoot length (6.80 ± 0.12 cm)/explant at fourth subculture passage, which a decline in shoot proliferation was recorded. Different concentrations of NAA were tested for ex vitro rooting of microshoots. The maximum percentage of rooting 100 % with maximum roots (18.3 ± 0.1) was achieved in soilrite when basal portion of the microshoots were treated with 200 μM (NAA) for 15 min per shoot. The plantlets went through hardening phase in a growth chamber, prior to ex vitro transfer. The PCR-based single primer amplification reaction (SPAR) methods which include random amplified polymorphic DNA (RAPD) and direct amplification of minisatellite DNA (DAMD) markers has been used for assessment of genetic stability of micropropagated plantlets. No variation was observed in DNA fingerprinting patterns among the micropropagated and the donor plants illustrating their genetic uniformity.

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“…A RAPD-based homogeneity analysis has been successfully used in Zingiber officinales (Rout et al 1998), Camellia spp. (Borchetia et al 2009), A. andraeanum (Gantait et al 2012), and Musa sp. (Punyarani et al 2013).…”

Section: Discussionmentioning

confidence: 99%

Direct somatic embryogenesis and genetic homogeneity assessment of regenerated plants of Anthurium andraeanum Linden cv. Fantasia

Bhattacharya

Dam

Karmakar

et al. 2016

In Vitro Cell.Dev.Biol.-Plant

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We present here a method for the in vitro propagation of Anthurium andraeanum cv. Fantasia through direct somatic embryogenesis. The embryos were directly formed at the cut end of most of the leaf explants when cultured on MS basal medium supplemented with N 6 -benzyladenine (BA) plus α-naphthalene acetic acid (NAA). MS basal media supplemented with BA (0.27 μM) and NAA (2.68 μM) induced maximum number of somatic embryos per leaf explant (15.33 ± 1.45) after 28 d of continuous culture. The highest numbers of embryos (12.33 ± 0.88) were matured into plantlets in MS basal medium augmented with 0.27 μM BA and 58.4 mM sucrose. Histology showed the presence of scutellum, coleoptile, welldeveloped root, and shoot initials at different stages of somatic embryo (SE) development directly on leaf explants. About 85% of the plantlets were successfully acclimatized, and of these, 80.3% plants survived after secondary hardening under greenhouse conditions. The embryo-derived plants successfully flowered. The presence of monomorphic DNA bands in random amplified polymorphic DNA (RAPD) marker analysis confirmed the genetic homogeneity of direct somatic embryo-derived plantlets in respect to their mother plant.

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High multiplication frequency and genetic stability for commercialization of the three varieties of micropropagated tea plants (Camellia spp.)

Cited by 36 publications

References 11 publications

In vitro plant regeneration of Albizia lebbeck (L.) from seed explants

In vitro plant regeneration of Albizia lebbeck (L.) from seed explants

Interactive Effects of Growth Regulators, Carbon Sources, pH on Plant Regeneration and Assessment of Genetic Fidelity Using Single Primer Amplification Reaction (SPARS) Techniques in Withania somnifera L.

Direct somatic embryogenesis and genetic homogeneity assessment of regenerated plants of Anthurium andraeanum Linden cv. Fantasia

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