High NESTIN Expression Marks the Endosteal Capillary Network in Human Bone Marrow

Panvini, Francesca M.; Pacini, Simone; Montali, Marina; Barachini, Serena; Mazzoni, Stefano; Morganti, Riccardo; Ciancia, Eugenio; Carnicelli, Vittoria; Petrini, Mario

doi:10.3389/fcell.2020.596452

Cited by 12 publications

(7 citation statements)

References 64 publications

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“…13,28 The human BM vascular microarchitecture has been described in detail. 29 Like previous observations, we find nestin in endothelial cells of small arterioles and capillaries 30 and sub-endothelial perivascular cells. Nestin is upregulated in endothelial cells and in pericytes following BM transplant by IHC (Figure 1).…”

Section: Discussionsupporting

confidence: 89%

Nestin expression in osteocytes following myeloablation and during bone marrow metastasis

Koerber

Schneider²,

Pritchard³

et al. 2022

Br J Haematol

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Nestin, originally described in neuroepithelial stem cells, 1 is an intermediate filament expressed during early stages of development. Its expression inversely correlates with cellular differentiation. In the bone marrow (BM), nestin is expressed in perivascular mesenchymal stem cells (MSCs) which contribute to bone development and are in direct contact with haematopoietic stem cells (HSCs). MSCs are multipotent cells capable of differentiating into osteoblastic, chondrocytic and adipocytic cells. Their presence in the BM was first described by Caplan. 2 Subsequently, nestin was broadly used as an MSC and HSC marker in the BM with little attention as to its expression pattern in mature osteocytes. Notably, researchers found distinct nestin + MSCs distributed around blood vessels. 3 Based on cell fluorescence intensity and cellular morphology in nestin fluorescent protein fusion protein tagged (Nes-GFP) transgenic mice, two distinct types of Nes-GFP+ mesenchymal progenitor cell types (Nes-GFPbright and Nes-GFPdim) were identified. Nes-GFPbright cells contained most CFU-Fs and a higher expression of genes which are associated with the HSC niche. By contrast, DNA replication and cell cycle pathways were significantly enriched in

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Section: Discussionsupporting

confidence: 89%

Nestin expression in osteocytes following myeloablation and during bone marrow metastasis

Koerber

Schneider²,

Pritchard³

et al. 2022

Br J Haematol

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“…CD271 stains for marrow stromal progenitor cells (37, 39, 40). CXCL12 and Nestin have also been shown to play a role in hematopoietic support within the human BM stromal compartment (41, 42). Using paraffin-embedded samples stored from the diagnostic workup of our patients, we stained consecutive sections and examined the presence of positive cells.…”

Section: Resultsmentioning

confidence: 99%

Adrenal extramedullary hematopoiesis as an inducible model of the adult hematopoietic niche

Schyrr

Alonso-Calleja

Vijaykumar

et al. 2023

Preprint

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Hematopoietic Stem and Progenitor Cells (HSPCs) reside in the hematopoietic niche, a structure that regulates the balance of cellular quiescence, self-renewal and commitment in a demand-adapted manner. The bone marrow (BM) hematopoietic niche is formed by several cellular players, mainly endothelial cells, osteoblasts, adipocytes, and stromal cells. While the BM niche forms a complex structure, evidence exists for simpler, albeit functional, extramedullary hematopoietic niches. However, the composition of what constitutes the simplest unit of an HSPC supportive microenvironment remains largely unknown. Here, we show that the adult adrenal gland can be transformed into a hematopoietic supportive environment. Upon splenectomy and hormonal stimulation, the adult adrenal gland can be induced to recruit and host HSPC function, including serial transplantation. Furthermore, the adrenal stroma contains a CXCL12+ population, reminiscent of BM CXCL12-Abundant Reticular (CAR) cells. Mirroring this, we found CXCL12+ cells in patient samples obtained from a local cohort of myelolipoma, a benign adrenal tumor composed of adipose and hematopoietic tissue that constitutes the most common site of extramedullary hematopoiesis specific to the adult. We present our model as a novel tool to increase our understanding of the physiology of hematopoietic support and to facilitate the development of a boneless niche model.

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“…Previous studies used MSC derived from different sources originated from mesoderm or extraembryonic tissue, such as BM, umbilical cord, adipose tissue, and placenta, in the therapeutic field as well as for hematologic disorders [ 10 ]. In this sense, the current studies have shown that nestin-positive mesenchymal stem cells (MSCs) are not only part of the HSC niche but are also required for HSC maintenance and hematopoiesis [ 52 , 53 ]. Based on this, we developed a novel and disruptive technology to isolate human immature dental pulp stem cells (hIDPSCs)—a special type of MSC that expresses high levels of nestin [ 19 ].…”

Section: Discussionmentioning

confidence: 99%

Therapeutic Potential of Human Immature Dental Pulp Stem Cells Observed in Mouse Model for Acquired Aplastic Anemia

Gonzaga

Wenceslau²,

Vieira

et al. 2022

Cells

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Aplastic anemia (AA) is a rare and serious disorder of hematopoietic stem cells (HSCs) that results in the loss of blood cells due to the failure of the bone marrow (BM). Although BM transplantation is used to treat AA, its use is limited by donor availability. In this sense, mesenchymal stem cells (MSCs) can offer a novel therapeutic approach for AA. This is because the MSCs contribute to the hematopoietic niche organization through their repopulating. In our study, we used the human immature dental pulp stem cell (hIDPSC), an MSC-like cell, to explore an alternative therapeutic approach for AA. For this, isogenic C57BL/6 mice were exposed to total body irradiation (TBI) to induce the AA. After 48 h of TBI, the mice were intraperitoneally treated with hIDPSC. The immunohistochemistry analyses confirmed that the hIDPSCs migrated and grafted in the mouse bone marrow (BM) and spleen, providing rapid support to hematopoiesis recovery compared to the group exposed to radiation, but not to those treated with the cells as well as the hematological parameters. Six months after the last hIDPSC transplantation, the BM showed long-term stable hematopoiesis. Our data highlight the therapeutic plasticity and hematoprotective role of hIDPSC for AA and potentially for other hematopoietic failures.

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High NESTIN Expression Marks the Endosteal Capillary Network in Human Bone Marrow

Cited by 12 publications

References 64 publications

Nestin expression in osteocytes following myeloablation and during bone marrow metastasis

Nestin expression in osteocytes following myeloablation and during bone marrow metastasis

Adrenal extramedullary hematopoiesis as an inducible model of the adult hematopoietic niche

Therapeutic Potential of Human Immature Dental Pulp Stem Cells Observed in Mouse Model for Acquired Aplastic Anemia

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