High-Performace Liquid Chromatographic Determination of Mono- and Oligosaccharides in Vegetables with Evaporative Light-Scattering Detection and Refractive Index Detection

Abstract

Two high-performance liquid chromatographic methods are compared for the determination of mono-and oligosaccharides in vegetables using an NH2 column and acetonitrile-water as the mobile phase. One method uses a gradient elution and evaporative light-scattering detection; method precision (relative standard deviation) ranges from 1.09 to 4.44%, and detection limits range from 0.014 to 0.061 mg/mL. The second method uses isocratic elution and refractive index detection; method precision (relative standard deviation) ranges from 1.16 to 4.34%, and detection limits range from 0.013 to 0.022 mg/mL. When samples are analyzed by both methods, the difference between the two mean values is not statistically significant.

Physicochemical properties and antimicrobial activity of Roselle (Hibiscus sabdariffa L.)

“…Sugar was analyzed using high‐performance liquid chromatography (HPLC), as previously described by Hernandez et al . HPLC analysis of ascorbic acid was modified from the procedure of Lee et al .…”

“…Roselle‐based products are used in popular medicine to obtain an antihypertensive effect as well as to prevent cardiovascular and hepatic disease . Roselle petals are potentially a good source for antioxidant agents such as anthocyanins and ascorbic acid . Food products such as fruit juices and vegetables, or dietary supplements such as vitamins are frequently employed for their preventive action because of the immediate effect on the redox status of body fluids.…”

Antioxidant profile, antioxidant activity, and physicochemical characteristics of strawberries from different cultivars and harvest locations

“…The quantification of sugars was performed using the method as described by Hernández et al (1998) with some modifications. Strawberry extracts were diluted tenfold in distilled water and then the samples were filtered through a syringe filter of 0.45 lm for high-performance liquid chromatography (HPLC) analyses.…”

Enzymatic process of rice bran: a stabilized functional food with nutraceuticals and nutrients

“…The quantification of the end product of complex carbohydrates of enzyme treated RB were performed by HPLC (Shimadzu LC, Japan) using aminopropyl column (150 × 4.6 mm) with refractive index detector (RID-10A) (Hernandez et al 1998). The end products were quantified with respective standards.…”