High-performance immune diagnosis of tuberculosis: Use of phage display and synthetic peptide in an optimized experimental design

Serena, Natália Notto; Boschero, Raphael Aparecido; Santiani, Manuel Hospinal; Pacce, Violetta Dias; Vedova-Costa, Jean Michel Dela; Magalhães, None Francisco Beraldi De; Wiedmar, Carlos; Alban, Silvana Maria; Soccol, Carlos Ricardo; Soccol, Vanete Thomaz

doi:10.1016/j.jim.2022.113242

Cited by 5 publications

(3 citation statements)

References 38 publications

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“…have identified new peptides of M. tuberculosis and harnessed phage display peptide libraries to screen IgG antibodies in TB-infected patients. One specific peptide, designated as P.Mt.PD.7, demonstrated significant promise and was adapted for use in enzyme-linked immunosorbent assays (ELISA), exhibiting 100% sensitivity and specificity [ 55 ]. A variety of reporter genes have also been incorporated into mycobacteriophages to enhance diagnostic capabilities.…”

Section: Mycobacterium Tuberculosismentioning

confidence: 99%

Bacteriophage-based bioassays: an expected paradigm shift in microbial diagnostics

Wahid,

Tiwana

2024

Future Microbiology

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Bacteriophages, as abundant and specific agents, hold significant promise as a solution to combat the growing threat of antimicrobial resistance. Their unique ability to selectively lyse bacterial cells without harming humans makes them a compelling alternative to traditional antibiotics and point-of-care diagnostics. The article reviews the current landscape of diagnostic technologies, identify gaps and highlight emerging possibilities demonstrates a comprehensive approach to advancing clinical diagnosis of microbial pathogens and covers an overview of existing phage-based bioassays. Overall, the provided data in this review effectively communicates the potential of bacteriophages in transforming therapeutic and diagnostic paradigms, offering a holistic perspective on the benefits and opportunities they present in combating microbial infections and enhancing public health.

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Section: Mycobacterium Tuberculosismentioning

confidence: 99%

Bacteriophage-based bioassays: an expected paradigm shift in microbial diagnostics

Wahid,

Tiwana

2024

Future Microbiology

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“…There are other ways to increase the effi ciency of serological tests to determine anti-TB antibodies today. For example, increasing the number of antigens used in the test (before Jacobs R, et al (2022) [14]) in combination with the defi nition of infl ammatory markers, search and the use of several MBT mimotopes to determine the corresponding IgG in the blood of patients [12].…”

Section: Antibiotic Resistance Typesmentioning

confidence: 99%

“…In particular, it is promising to determine circulating microRNAs (miRNAs) as a potential biomarker to detect tuberculosis [9], immunoassays for measuring the reduced CD27 expression in T-cells in blood [10], and determining complex metabolites in the blood using nuclear-magnetic resonance method [11]. Brazilian scientists used new MBT peptide mimotopes for serodiagnosis of TB (IgG antibodies) and achieved 100% sensitivity and specifi city when tested on a 100-sera panel [12]. This is a practical and cost-eff ective approach suitable for large-scale TB screening.…”

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confidence: 99%

Detection of Specific Antibodies Using Mpt83-Mpt63 Fusion Protein in Patients With Destructive Pulmonary Tuberculosis

Rekalova

Panasiukova²,

Pohrebna³

et al. 2022

PSSS MS

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Introduction. The development of eff ective test systems for tuberculosis (TB) diagnosis and the study of factors infl uencing their sensitivity and specifi city constitute urgent tasks given the unstable epidemiological situation with TB in Ukraine and the world. The study aimed to determine the clinical and laboratory features of pulmonary TB that may aff ect the sensitivity of IgG TB antibody detection in human serum by the immuno-enzyme test system "IB-Chem Anti-Mycobacterium tuberculosis", which was developed at the Palladin Institute of Biochemistry of the NAS of Ukraine and uses recombinant fusion protein MPT83-MPT63 as an antigen. Methods. Sera of 62 patients with chemoresistant pulmonary TB were tested for TB antibodies using an indirect enzyme-linked immunosorbent assay. Dependence of the obtained results on clinical symptoms and signs of pulmonary TB, the frequency of diff erent types of antibiotic resistance, cellular and biochemical parameters of blood were analysed. Results. The sensitivity of the immuno-enzyme test system "IB-Chem Anti-Mycobacterium tuberculosis" for detecting human IgG TB antibodies using recombinant fusion protein MPT83-MPT63 was found to constitute 71.0%; it reached 81.3% in patients with destructive pulmonary TB. Conclusion. The developed test system should primarily be used for the TB diagnosis in patients with destructive changes in the lungs where the diagnostic effi ciency of this test system reaches high indicators.

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The use of peptides for immunodiagnosis of human Chagas disease

Ribeiro,

Silva,

Lopes

et al. 2024

Amino Acids

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Chagas disease, caused by the protozoa Trypanosoma cruzi, continues to be a serious public health problem in Latin America, worsened by the limitations in its detection. Given the importance of developing new diagnostic methods for this disease, the present review aimed to verify the number of publications dedicated to research on peptides that demonstrate their usefulness in serodiagnosis. To this end, a bibliographic survey was conducted on the PubMed platform using the keyword “peptide” or “epitope” combined with “Chagas disease” or “Trypanosoma cruzi”; “diagno*” or “serodiagnosis” or “immunodiagnosis”, without period restriction. An increasing number of publications on studies employing peptides in ELISA and rapid tests assays was verified, which confirms the expansion of research in this field. It is possible to observe that many of the peptides tested so far originate from proteins widely used in the diagnosis of Chagas, and many of them are part of commercial tests developed. In this sense, as expected, promising results were obtained for several peptides when tested in ELISA, as many of them exhibited sensitivity and specificity values above 90%. Furthermore, some peptides have been tested in several studies, confirming their diagnostic potential. Despite the promising results observed, it is possible to emphasize the need for extensive testing of peptides, using different serological panels, in order to confirm their potential. The importance of producing an effective assay capable of detecting the clinical stages of the disease, as well as new immunogenic antigens that enable new serological diagnostic tools for Chagas disease, is evident.

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High-performance immune diagnosis of tuberculosis: Use of phage display and synthetic peptide in an optimized experimental design

Cited by 5 publications

References 38 publications

Bacteriophage-based bioassays: an expected paradigm shift in microbial diagnostics

Bacteriophage-based bioassays: an expected paradigm shift in microbial diagnostics

Detection of Specific Antibodies Using Mpt83-Mpt63 Fusion Protein in Patients With Destructive Pulmonary Tuberculosis

The use of peptides for immunodiagnosis of human Chagas disease

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