High-performance liquid chromatographic analysis of even- and odd-numbered hyaluronate oligosaccharides

Functionalized Derivatives of Hyaluronic Acid Oligosaccharides: Drug Carriers and Novel Biomaterials

“…A column profile was generated based on these data (Figure 3a), and the fractions corresponding to each number of disaccharide units were pooled. Further characterization of the fragments was accomplished by HPLC on an aminomodified silica gel column using 0.1 M KH2PC>4 buffer (pH = 4.75) as the mobile phase (21). This column allows the separation of HA fragments up to six disaccharide units.…”

“…All solvents used were Optima (HPLC grade), obtained from Fisher Chemical Co. (Pittsburgh, PA). HA fragments of defined length were generated by either limited or exhaustive digestion with testicular hyaluronidase and were separated, purified, and characterized according to published procedures (19)(20)(21). HA oligosaccharides were assayed using the carbazole method (22) with glucuronolactone as the standard.…”

Quality control of herbal medicines

“…On the other hand, the advantages of HPLC lie in its versatility for the analysis of the chemical compounds in herbal medicines, However, the commonly used detector in HPLC, say single wavelength UV detector, seems to be unable to fulfill the task, since lots of chemical compounds in herbal medicines are non-chromophoric compounds. Consequently, a marked increase in the use of HPLC analysis coupled with evaporative light scattering detection (ELSD) in a recent decade demonstrated that ELSD is an excellent detection method for the analysis of non-chromophoric compounds [82][83][84]. This new detector provides a possibility for the direct HPLC analysis of many pharmacologically active components in herbal medicines, since the response of ELSD depends only on the size, shape, and number of eluate particles rather than the analysis structure and/or chromophore of analytes as UV detector does.…”