High-performance liquid chromatographic analysis of retinal and retinol isomers

Nöll, Gottfried N.

doi:10.1016/0021-9673(95)00789-x

Cited by 56 publications

(23 citation statements)

References 22 publications

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“…The HPLC system consisted of a Shimadzu LC‐9 A pump, a Shimadzu SIL‐6B/9 A autoinjector, a Shimadzu SPD‐2 A UV‐detector at 325 nm, an integrator (Turbochrom, version 4.0, Perkin‐Elmer, Cupertino, CA, USA), a Hichrom Hypersil column (4.6 × 150 mm, 3 μm particle size) and a 90:10 hexane:isopropanol mobile phase. This procedure was validated for fish organ homogenates where standard additions showed identification, precision, linearity and trueness of the method in line with descriptions from Nöll (1996). Dry matter content of the diets and fish samples were measured gravimetrically after freeze‐drying.…”

Section: Analysessupporting

confidence: 62%

“…Vitamins A 1 and A 2 were measured in all diets, and whole body and liver homogenates using an HPLC procedure modified from Nöll (1996). The HPLC system consisted of a Shimadzu LC‐9 A pump, a Shimadzu SIL‐6B/9 A autoinjector, a Shimadzu SPD‐2 A UV‐detector at 325 nm, an integrator (Turbochrom, version 4.0, Perkin‐Elmer, Cupertino, CA, USA), a Hichrom Hypersil column (4.6 × 150 mm, 3 μm particle size) and a 90:10 hexane:isopropanol mobile phase.…”

Section: Analysesmentioning

confidence: 99%

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Hypervitaminosis A in first-feeding fry of the Atlantic salmon (Salmo salar L.)

Ørnsrud¹,

Graff²,

Høie³

et al. 2002

Aquaculture Nutrition

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Atlantic salmon fry were reared on a fishmeal based diet with increasing levels of vitamin A (VA) (6, 122 and 938 mg retinol kg–1 dry feed) from startfeeding and for 14 weeks. Signs of VA stress, such as reduced fat stores, liver size and growth, were found for groups receiving 122 and 938 mg retinol kg–1. Signs of vitamin A toxicity, such as increased mortality, abnormal vertebral growth, and reduced growth, were found for groups receiving 938 mg retinol kg–1. These results suggest that excess VA in the early life stages of Atlantic salmon is deleterious for normal development.

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Section: Analysessupporting

confidence: 62%

Section: Analysesmentioning

confidence: 99%

Hypervitaminosis A in first-feeding fry of the Atlantic salmon (Salmo salar L.)

Ørnsrud¹,

Graff²,

Høie³

et al. 2002

Aquaculture Nutrition

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“…The fatty acid composition in the oil was analyzed by gas liquid chromatography (Trace GC 2000) according to previously described methods,17,22 with some modifications; the fatty acids were esterified in 20% boron fluoride (BF 3 ) in methanol, and sample parallels were analyzed. The level of fat soluble vitamins A23,24 and E25 was analyzed by high-performance liquid chromatography, and the lipid peroxidation was analyzed by thiobarbituric acid reactive substances26,27 (Table 1). The seal oil was added with a combination of natural and synthetic tocopherols, the latter being dl-α tocopheryl acetate.…”

Section: Methodsmentioning

confidence: 99%

Duodenal administered seal oil for patients with subjective food hypersensitivity: an explorative open pilot study

Gregersen

Lind²,

Valeur³

et al. 2010

IJGM

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Short-term duodenal administration of n-3 polyunsaturated fatty acid (PUFA)-rich seal oil may improve gastrointestinal complaints in patients with subjective food hypersensitivity, as well as joint pain in patients with inflammatory bowel disease (IBD). The aim of the present explorative pilot study was to investigate whether 10-day open treatment with seal oil, 10 mL self-administrated via a nasoduodenal tube 3 times daily, could also benefit nongastrointestinal complaints and quality of life (QoL) in patients with subjective food hypersensitivity. Twenty-six patients with subjective food hypersensitivity, of whom 25 had irritable bowel syndrome (IBS), were included in the present study. Before and after treatment and 1 month posttreatment, patients filled in the Ulcer Esophagitis Subjective Symptoms Scale (UESS) and the Gastrointestinal Symptom Rating Scale (GSRS) for gastrointestinal symptoms and subjective health complaints (SHC) inventory for nongastrointestinal symptoms in addition to short form of the Nepean dyspepsia index (SF-NDI) for evaluation of QoL. Compared with baseline, gastrointestinal, as well as nongastrointestinal, complaints and QoL improved significantly, both at end of treatment and 1 month posttreatment. The consistent improvements following seal oil administration warrant further placebo-controlled trials for confirmation of effect.

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“…• C prior to analysis of fatty acid composition and didehydro-all-trans retinol (A 2 ) in marine oils was analysed by a modified HPLC method [36,37]. Briefly, the sample is saponified, while the unsaponified sample material is extracted, and analysed by a HPLC column (HICHROM 4,6  150 mm, LC-SI, 3m, Teknolab A/S) using UV-detector (Thermo Separations products, UV1000, Instrument-Teknikk AS) with reference to an external standard curve.…”

Section: Experimental Oilsmentioning

confidence: 99%