High performance liquid chromatographic assay of cefsulodin, cefotiam and cefmenoxime in serum and urine.

Itakura, Koichi; Mitani, Masayoshi; Aoki, Isamu; Usui, Yoshiro

doi:10.1248/cpb.30.622

Cited by 11 publications

(2 citation statements)

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“…Two RP-HPLC methods were reported during this review period for quantifying cefotiam and other cephalosporins after deproteinization (37,44). An LC method involved the simultaneous determination of cefotiam and cefsulodin in serum and bone marrow (75).…”

Section: Methodsmentioning

confidence: 99%

Recent analytical methods for cephalosporins in biological fluids

Toothaker¹,

Wright²,

Pachla³

1987

Antimicrob Agents Chemother

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Since 1980, RP chromatography has been the principal analytical technique used for cephalosporins. This technology offers selectivity, accuracy, and ease of use. Most of the methods rely on protein precipitation and, to a lesser extent, solid-phase isolation or extraction procedures. The proper selection of a method depends on the analytical constraints imposed by the overall objective of the study. For example, pharmacokinetic datum interpretation mandates that the method be validated and provide specific and accurate results. LC is the preferred technique, since it not only meets these specifications but may also distinguish between the drug and metabolites. Those chromatographic methods which quantify several different cephalosporins are not desirable for pharmacokinetic datum interpretation, since accuracy and precision are usually compromised in order that many different drugs may be quantified in a single analysis. The proper selection of sample preparation method is dependent on the presence of potential interferences and the acceptable lower limit of quantitation. Protein precipitation methods offer ease of sample preparation but may suffer from nonselectivity. Solid-phase isolation and extraction procedures may increase selectivity and improve the limit of quantitation. Although LC provides specific and accurate results, clinical laboratories may prefer to use the less specific methods for therapeutic drug monitoring. In this case, microbiological, enzymatic, and fluorimetric methods offer improved sample throughput but less specificity. However, these methods should not be used for drugs that may have a low margin of safety or if the patient is on multiple-antibiotic therapy. Future methods may involve incorporating solid-phase isolation columns to enhance the specificity of chromatographic, microbiological, enzymatic, and fluorescence methods. Advancements in microbore column technology may allow improvements in the selectivity and sensitivity of LC methods. Many investigators prefer to use simple protein precipitation procedures for sample preparation because of sample throughput constraints. However, advances in robotic sample preparation may allow the more cumbersome solid-phase isolation or extraction techniques to be used to improved sample throughput and specificity.

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Section: Methodsmentioning

confidence: 99%

Recent analytical methods for cephalosporins in biological fluids

Toothaker¹,

Wright²,

Pachla³

1987

Antimicrob Agents Chemother

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“…Bacillus subtilis A TCC 6633 or Proteus mirabilis A TCC 21100 are generally employed as test organisms in the plate agar diffusion method, and the limit of quantitation is about 0.5 to I mg/L (Fugono & Maeda 1979). The use of high performance liquid chromatography (Fourtillan 1986;Fourtillan et al 1982;Itakura et al 1982;Lecaillon et al 1983) with UV detection at 254nm results in lower detection limits (0.1 to 0.3 mg/L).…”

Section: General Characteristicsmentioning

confidence: 99%

Clinical Pharmacokinetics of Cefotiam

Jehl²,

Willemin³

et al. 1989

Clinical Pharmacokinetics

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Cefotiam, a semisynthetic parenteral cephalosporin of the aminothiazole group, exhibits interesting properties: stability against hepatic metabolism and excellent solubility, accounting for an apparent volume of distribution 2 to 3 times higher than that of most other cephalosporins. Its degree of protein binding is about 40%. High concentrations of cefotiam are observed in several tissues (kidney, heart, ear, prostate and genital tract) as well as in fluids and secretions (bile, ascitic fluid). In healthy subjects, the serum elimination half-life is about 1 hour. The pharmacokinetics are linear only for doses lower than 1g. Cefotiam is mostly (and rapidly) eliminated in unchanged form in urine; 50 to 70% of the dose is recovered during the 12 hours following administration, and only severe renal failure, with creatinine clearance less than 5 ml/min, significantly alters the elimination half-life. Although the drug has no proven nephrotoxicity in man, a reduction of the dose is recommended when creatinine clearance is less than 30 ml/min.

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Antibiotic monitoring in body fluids

Rouan¹

1985

Journal of Chromatography B: Biomedical Sciences and Applicatio

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High performance liquid chromatographic assay of cefsulodin, cefotiam and cefmenoxime in serum and urine.

Cited by 11 publications

References 0 publications

Recent analytical methods for cephalosporins in biological fluids

Recent analytical methods for cephalosporins in biological fluids

Clinical Pharmacokinetics of Cefotiam

Antibiotic monitoring in body fluids

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