High-performance liquid chromatographic column switching techniques for rapid hydrocarbon group-type separations

Alfredson, Thomas V.

doi:10.1016/s0021-9673(00)82097-x

Cited by 64 publications

(13 citation statements)

References 19 publications

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“…Since nitrobenzene is not present in the sample of Figure 7, it is necessarily excluded from these calculations. 6 Calculated according to eq 14.…”

Section: Results and Discussion Chromatographic Expectations And Expe...mentioning

confidence: 99%

“…Isoelectric focusing/gel electrophoresis perhaps best exemplifies the advantages of multidimensional separations by providing peak capacities in the thousands (2). More recently, multidimensional separations have been applied to column chromatography, i.e., GC/GC (3,4), HPLC/HPLC (5)(6)(7), and HPLC/GC (8)(9)(10), with a reasonable amount of success. However, there has been some degree of difficulty in interfacing column chromatographic dimensions (11), and the additional instrumentation or instrumental modifications can be costly.…”

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confidence: 99%

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Sequential multimodal elution for pseudomultidimensional liquid chromatography on a single column

Little¹,

Jeansonne²,

Foley³

1991

Anal. Chem.

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A method of elution is described that yields significantly higher (2-3 times) peak capacities and facilitates the separation of compounds by class (e.g., acids versus neutrals), thereby increasing the Informing power and the selectivity. The method Is based on the sequential application of two or more elution modes, all of which, except the last one, are selective, I.e., designed to elute only a certain class of compounds. The total peak capacity Is given approximately by " where r Is the number of elution modes and , Is the peak capacity of an individual elution mode, roughly equal to that obtained In a conventional one-dimensional separation. The Increase In information content Is proportional to log r but Is also dependent on the relative proportion of sample components eluted during Individual separation modes. Several reversed-phase examples utilizing sequential pH and solvent gradients are presented, including separations of benzoic acids from neutral aromatic compounds, peptides from neutral species, and phenols from poly aromatic hydrocarbons. Solute bandwidths, retention time, and area reproducibility are comparable to that of conventional reversed-phase separations.Although the resolving power of this multimodal, single-column elution method Is somewhat less than that of a true two-dimensional (multiple column) method, It can be an effective and simple alternative for the analysis of moderately complex samples.

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“…Since nitrobenzene is not present in the sample of Figure 7, it is necessarily excluded from these calculations. 6 Calculated according to eq 14.…”

Section: Results and Discussion Chromatographic Expectations And Expe...mentioning

confidence: 99%

mentioning

confidence: 99%

Sequential multimodal elution for pseudomultidimensional liquid chromatography on a single column

Little¹,

Jeansonne²,

Foley³

1991

Anal. Chem.

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show abstract

“…By this means the size of the transferred zone is minimized and reconcentration of the transferred zone on top of and polar bonded phase and normal phase chromatography [294][295][296][297]. To bring about a significant improvement in resolution the retention mechanisms for the two columns should be complementary.…”

Section: Multidimensional Liquid Chromatographymentioning

confidence: 99%

Sample Preparation for Chromatographic Analysis

Poole

1991

Chromatography Today

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“…This last approach was widely utilized in LC during the last two decades of the past century in order to characterize liquid fuels through a group‐type fractionation (such as paraffins, olefins, naphtenes, aromatics, and polars). PONA and related approaches usually utilize three columns–a highly activated silica column to retard the polar compounds in the first column; a silica impregnated with silver ions to retain the olefins in the second column and a cyano or amino‐silica column to determine the saturated and aromatic hydrocarbon classes . However, the utilization of 3D LC became very scarce in the present century, with a clear predominance of 2D separations.…”

Section: Introductionmentioning

confidence: 99%

An overview of multidimensional liquid phase separations in food analysis

et al. 2016

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Food safety is a priority public health concern that demands analytical methods capable to detect low concentration level of contaminants (e.g. pesticides and antibiotics) in different food matrices. Due to the high complexity of these matrices, a sample preparation step is in most cases mandatory to achieve satisfactory results being usually tedious, lengthy, and prone to the introduction of errors. For this reason, many research groups have focused efforts on the development of online systems capable to do the cleanup, concentration, and separation steps at once through multidimensional separation techniques (MDS). Among several possible setups, the most popular are the multidimensional chromatographic techniques (MDC) that consist in combining more than one mobile and/or stationary phase to provide a satisfactory separation. In the present review, we selected a variety of multidimensional separation systems used for food contaminant analysis in order to discuss the instrumentation aspects, the concept of orthogonality, column approaches used in these systems, and new materials that can be used in these columns. Selected classes of contaminants present in food matrices are introduced and discussed as example of the potential applications of multidimensional liquid phase separation techniques in food safety.

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High-performance liquid chromatographic column switching techniques for rapid hydrocarbon group-type separations

Cited by 64 publications

References 19 publications

Sequential multimodal elution for pseudomultidimensional liquid chromatography on a single column

Sequential multimodal elution for pseudomultidimensional liquid chromatography on a single column

Sample Preparation for Chromatographic Analysis

An overview of multidimensional liquid phase separations in food analysis

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