High performance liquid chromatographic determination of aflatoxins in chilli, peanut and rice using silica based monolithic column

Khayoon, Wejdan Shakir; Saad, Bahruddin; Lee, Tien Ping; Salleh, Baharuddin

doi:10.1016/j.foodchem.2012.01.010

Cited by 81 publications

(28 citation statements)

References 27 publications

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“…The austere limits give demand for availability of sensitive methods with limits of detection low enough. Currently, high performance liquid chromatography (Khayoon et al 2012) and enzyme linked immunosorbent assay (Durakovic et al 2012) are the most common methods for aflatoxins assay. The methods have some drawbacks such as acquisition and operating costs, and time consuming protocols.…”

Section: Introductionmentioning

confidence: 99%

Spectrophotomeric Assay of Aflatoxin B1 Using Acetylcholinesterase Immobilized on Standard Microplates

Pohanka

2013

Analytical Letters

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Aflatoxins are a group of hepatotoxic secondary metabolites produced by molds Aspergillus. During inappropriate storage or processing of food and beverages, aflatoxins can be distributed and become a serious health problem. Disparate analytical techniques were prepared for the assay in the past. Here, a method based on inhibition of enzyme acetylcholinesterase (AChE) is performed allowing fast and simple prove of aflatoxins presence. For the assay purposes, AChE was immobilized on standard polystyrene microplates using gelatin as a stabilizing substance. Both free and immobilized AChEs were used and compared one to each other. Immobilization protocol was optimized and interference of organic solvents such as methanol was tested. For the immobilized AChE, limit of detection 2.75 ppb for aflatoxin B1 was received. The immobilized AChE kept full activity at least one month. Suitability of the assay for a practical performance is considered.

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Section: Introductionmentioning

confidence: 99%

Spectrophotomeric Assay of Aflatoxin B1 Using Acetylcholinesterase Immobilized on Standard Microplates

Pohanka

2013

Analytical Letters

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“…Food/Feed (number of sample, n) M y c o t o x i n n positive, n above limit Reference Commercial rice (n = 5), peanut (n = 9), and chili (n = 10) Aflatoxins Rice n positive: 4/5 n above limit: 3/5 Peanut n positive: 4/9 n above limit: 2/9 Chili n positive: 9/10 n above limit: 3/10 Khayoon et al, 2012 Commercial coffee and cereal (n = 45) Ochratoxin A n positive: 25/45 n above limit: 1/45 Lee et al, 2012 Commercial cereal (n = 100) Aflatoxins B 1 , B 2 , G 1 , and G 2 , ochratoxin A, zearalenone, deoxynivalenol, and fumonisins B 1 , B 2 , T-2, and HT-2 AFs n positive: 60/100 n above limit: 2/100 OTA n positive: 40/100 n above limit: 2/100 ZEN n positive: 25/100 n above limit: None DON n positive: 36/100 n above limit: None FUM n positive: 19/100 n above limit: None T-2 n positive: 16/100 n above limit: None HT-2 n positive: 16/100 n above limit: None Soleimany, Jinap, & Abas, 2012 Commercial cereal (n = 80) Aflatoxins B 1 , B 2 , G 1 , and G 2 , ochratoxin A, zearalenone, deoxynivalenol, and fumonisins B 1 , B 2 , T-2, and HT-2 AFs n positive: 40/80 n above limit: 2/80 OTA n positive: 24/80 n above limit: 1/80 ZEN n positive: 15/80 n above limit: None DON n positive: 24/80 n above limit: None FUM n positive: 13/80 n above limit: None T-2 n positive: 11/80 n above limit: None HT-2 n positive: 9/80 n above limit: None Soleimany, Jinap, Faridah, & Khatib, 2012 Commercial food products (n = 95) Aflatoxin B 1 n positive: 69/95 n above limit: None Grain corn for animal feed (n = 80) Aflatoxin B 1 and fumonisins AFB 1 n positive: 65/80 n above limit: 18/80 FUM n positive: 80/80 n above limit: None Reddy & Salleh, 2011 (Continues) T A B L E 4 (Continued)…”

Section: Shuib Et Al 2017amentioning

confidence: 99%

Phytopathogenic organisms and mycotoxigenic fungi: Why do we control one and neglect the other? A biological control perspective in Malaysia

Yazid

Selamat

Ismail

et al. 2020

Comp Rev Food Sci Food Safe

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In this review, we present the current information on development and applications of biological control against phytopathogenic organisms as well as mycotoxigenic fungi in Malaysia as part of the integrated pest management (IPM) programs in a collective effort to achieve food security. Although the biological control of phytopathogenic organisms of economically important crops is well established and widely practiced in Malaysia with considerable success, the same cannot be said for mycotoxigenic fungi. This is surprising because the year round hot and humid Malaysian tropical climate is very conducive for the colonization of mycotoxigenic fungi and the potential contamination with mycotoxins. This suggests that less focus has been made on the control of mycotoxigenic species in the genera Aspergillus, Fusarium, and Penicillium in Malaysia, despite the food security and health implications of exposure to the mycotoxins produced by these species. At present, there is limited research in Malaysia related to biological control of the key mycotoxins, especially aflatoxins, Fusarium‐related mycotoxins, and ochratoxin A, in key food and feed chains. The expected threats of climate change, its impacts on both plant physiology and the proliferation of mycotoxigenic fungi, and the contamination of food and feed commodities with mycotoxins, including the discovery of masked mycotoxins, will pose significant new global challenges that will impact on mycotoxin management strategies in food and feed crops worldwide. Future research, especially in Malaysia, should urgently focus on these challenges to develop IPM strategies that include biological control for minimizing mycotoxins in economically important food and feed chains for the benefit of ensuring food safety and food security under climate change scenarios.

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“…Regarding the use of monolithic columns coupled to mass spectrometry, several LC-MS methods using this kind of column are being proposed, although it seems that the extremely high flow-rates generally applied in monoliths makes the compatibility with mass spectrometry detection difficult. Some food 85,86,92,96,100,105 and environmental 113,114,118 applications of monolithic columns to LC-MS can be found in the literature. For instance, Bignardi et al 96 compared the performance in terms of peak shape, resolution, analysis time and selectivity of both a C18 particle-packed column and a silica-based C18 monolithic silica column for the multi-allergen trace analysis in foods by liquid chromatography-electrospray ionization-linear ion traptandem mass spectrometry (LC-ESI-LIT-MS/MS).…”

Section: Food and Environmental Applicationsmentioning

confidence: 99%

Monolithic Columns in Fast Liquid Chromatography

Hara¹,

Núñez²,

Ikegami³

et al. 2015

Fast Liquid Chromatography–Mass Spectrometry Methods in Food and Environmental Analysis

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High performance liquid chromatographic determination of aflatoxins in chilli, peanut and rice using silica based monolithic column

Cited by 81 publications

References 27 publications

Spectrophotomeric Assay of Aflatoxin B1 Using Acetylcholinesterase Immobilized on Standard Microplates

Spectrophotomeric Assay of Aflatoxin B1 Using Acetylcholinesterase Immobilized on Standard Microplates

Phytopathogenic organisms and mycotoxigenic fungi: Why do we control one and neglect the other? A biological control perspective in Malaysia

Monolithic Columns in Fast Liquid Chromatography

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