High-performance liquid chromatographic determination of vertilmicin in rat plasma using sensitive fluorometric derivatization

Liu, Zhen; Sha, Yunfei; Huang, Taomin; Yang, Bei; Duan, Gengli

doi:10.1016/j.jchromb.2005.06.045

Cited by 12 publications

(8 citation statements)

References 20 publications

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“…In rats, after intravenous administration of vertilmicin at doses of 10, 20 and 40 mg/kg, the t 1/2 values for vertilmicin were within 0.76-0.86 h. The pharmacokinetic behaviors of vertilmicin were dose-dependent when the increasing dosages of vertilmicin were administered intravenously to rats. This result was similar with that reported by Liu et al (2005). Following a single intravenous administration of vertilmicin to rats, vertilmicin was rapidly distributed into various tissues, and was present in the kidney, uterus, and lung at higher concentrations at different time periods.…”

Section: Discussionsupporting

confidence: 92%

Pharmacokinetics of vertilmicin, a novel aminoglycoside antibiotic, in rats and dogs

Mao-jin

Mei-ying

Wan

et al. 2010

Eur J Drug Metab Pharmacokinet

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The pharmacokinetics of vertilmicin was investigated in rats and dogs following intramuscular or intravenous administration of vertilmicin. Following a single administration of an intramuscular dose, serum concentrations of vertilmicin peaked at 0.63 h in rats and 0.58 h in dogs. In rats, after intravenous administration of vertilmicin at dosages of 10, 20, and 40 mg/kg, the t1/2 values for vertilmicin were 0.81, 0.76, and 0.86 h, respectively, while after intramuscular administration of vertilmicin at dose of 20 mg/kg, the t1/2 value for vertiImicin was 0.79 h. In dogs, after intravenous or intramuscular administration of vertilmicin at a dose of 10 mg/kg, the t1/2 values for vertilmicin were 0.83 and 0.85 h, respectively. After intravenous dosing to rats vertilmicin was distributed to most organs and tissues, and kidney tissue exhibited the highest exposure, while the tissue with the lowest exposure was the brain. Following single intravenous administration of vertilmicin at a dose of 20 mg/kg to rats, about 81.1% of the vertilmicin was excreted in urine, while only 3.12 and 1.44% of the administered dose was excreted in feces and bile within 48 h. The mean values for the plasma protein binding of vertilmicin were 22.7 and 20.4% in rats and dogs, respectively. These results indicate that vertilmicin was rapidly absorbed and widely distributed into various tissues in rats. The pharmacokinetic behavior of vertilmicin was dose-dependent when increasing doses of vertilmicin were administered intravenously to rats. Renal excretion was the primary elimination route of vertilmicin following intravenous administration to rats.

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Section: Discussionsupporting

confidence: 92%

Pharmacokinetics of vertilmicin, a novel aminoglycoside antibiotic, in rats and dogs

Mao-jin

Mei-ying

Wan

et al. 2010

Eur J Drug Metab Pharmacokinet

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“…The determination of vertilmicin sulfate by reverse-phase HPLC after derivatization with 9-fluorenylmethyl chloroformate has been reported [22]. Optimal reaction of vertilmicin with FMOC-Cl occurred at pH 8.0 -8.5 and was complete at room temperature after 10 min.…”

Section: Selection Of Ismentioning

confidence: 99%

High performance liquid chromatography for the determination of glucosamine sulfate in human plasma after derivatization with 9‐fluorenylmethyl chloroformate

Huang

Deng

Chen

et al. 2006

J of Separation Science

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Original PaperHigh performance liquid chromatography for the determination of glucosamine sulfate in human plasma after derivatization with 9-fluorenylmethyl chloroformateIn this study, we developed a simple, rapid, sensitive, and reliable method for the determination of glucosamine sulfate in human plasma, which was based on derivatization with 9-fluorenylmethyl chloroformate (FMOC-Cl) followed by reverse-phase HPLC-FLD. For the first time, FMOC-Cl was introduced into derivatization of glucosamine sulfate in human plasma. The amino groups of glucosamine sulfate and vertilmicin sulfate (the internal standard) were trapped with FMOC-Cl to form glucosamine-FMOC-Cl and vertilmicin-FMOC-Cl adducts, which can be very suitable for HPLC-FLD. Precipitation of plasma proteins by acetonitrile was followed by vortex mixing and centrifugation. Chromatographic separation was performed on a C 18 column (DIAMONSIL 15064 mm id, 5 lm) with a mobile phase gradient consisting of acetonitrile and water at a flow-rate of 1 mL/min. The retention times of glucosamine-FMOC-Cl and vertilmicin-FMOC-Cl adducts were 8.9 and 21.2 min, respectively. This method was shown to be selective and sensitive for glucosamine sulfate. The limit of detection was 15 ng/mL for glucosamine sulfate in plasma and the linear range was 0.1 -10 mg/mL in plasma with a correlation coefficient (r) of 0.9999. The relative standard deviations (RSDs) of intra-day and inter-day assays were 5.2 -8.1% and 6.1 -8.5%, respectively. Extraction recoveries of glucosamine sulfate in plasma were greater than 90%. The validated method was successfully applied to the determination of glucosamine sulfate in human plasma samples.

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“…It was important to maintain a concentration of acetonitrile of around 60% v/v in the reaction mixture. FMOC-Cl was precipitated if the proportion of acetonitrile fell below 25% [17,18], while memantine hydrochloride and borate buffer are not dissoluble in higher acetonitrile proportion solution. A sufficiently high concentration of FMOC-Cl was required for the reaction to proceed efficiently.…”

Section: Optimization Of the Derivatization Conditionsmentioning

confidence: 99%

High‐performance liquid chromatographic determination of memantine hydrochloride in rat plasma using sensitive fluorometric derivatization

Xie

Zhou

Tong

et al. 2011

J of Separation Science

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In this study, we investigated a simple, sensitive and reliable liquid chromatography-fluorescence detection method for the determination of memantine hydrochloride in rat plasma which was based on derivatization with 9-fluorenylmethyl chloroformate (FMOC-Cl). For the first time, FMOC-Cl was introduced into derivatization of memantine hydrochloride in rat plasma. The amino groups of memantine hydrochloride and amantadine hydrochloride (internal standard) were trapped with FMOC-Cl to form memantine hydrochloride-FMOC-Cl and amantadine hydrochloride-FMOC-Cl compositions, which can be very compatible for LC-FLD. Precipitation of plasma proteins by acetonitrile was followed by vortex mixing and centrifugation. Chromatographic separation was performed on a C(18) column (DIAMONSIL 150 × 4.6 mm, id 5 μm) with a mobile phase consisting of acetonitrile and water at a flow rate of 1.0 mL/min. The retention times of memantine hydrochloride-FMOC-Cl and amantadine hydrochloride-FMOC-Cl compositions were 23.69 and 40.27 min, respectively. Optimal conditions for the derivatization of memantine hydrochloride were also described. The limit of quantification (LOQ) was 25 ng/mL for memantine hydrochloride in plasma, the linear range was 0.025-5.0 μg/mL in plasma with a correlation coefficient (r) of 0.9999. The relative standard deviations (RSDs) of intra-day and inter-day assays were 4.46-12.19 and 5.23-11.50%, respectively. The validated method was successfully applied to the determination of memantine hydrochloride in rat plasma samples.

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High-performance liquid chromatographic determination of vertilmicin in rat plasma using sensitive fluorometric derivatization

Cited by 12 publications

References 20 publications

Pharmacokinetics of vertilmicin, a novel aminoglycoside antibiotic, in rats and dogs

Pharmacokinetics of vertilmicin, a novel aminoglycoside antibiotic, in rats and dogs

High performance liquid chromatography for the determination of glucosamine sulfate in human plasma after derivatization with 9‐fluorenylmethyl chloroformate

High‐performance liquid chromatographic determination of memantine hydrochloride in rat plasma using sensitive fluorometric derivatization

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