High-performance liquid chromatographic determination of cefuroxime

Abstract

A rapid, precise and selective method is described for determining the purity of cefuroxime sodium. Cefuroxime is separated from closely related compounds by high-performance liquid chromatography a t ambient temperature using a 10-cm column packed with 5-,urn Spherisorb C6 and buffered aqueous acetonitrile as mobile phase. The eluate is monitored a t 273 nm, orcinol is used as internal standard and the method has a coefficient of variation of 0.6%. The procedure is suitable for the analysis of freshly manufactured cefuroxime sodium and it also provides a reliable estimate of the potency of partly degraded samples from stability tests.

Mechanistic investigation of the oxidation of Cefuroxime by hexacyanoferrate(III) in alkaline conditions

“…Atomic absorption procedure based on the reaction with silver nitrate/lead acetate at pH 7 [8] and, spectrophotometric methods after complexation with 7,7,8,8-tetracyanoquinodimethane, p-chloranilic acid, Prussian Blue and reaction with metol-chromium (VI) reagent [9,10] have been explored. Flow-injection analysis [11], capillary electrophoresis [12,13], liquid chromatography [14,15] and the analysis of injectible by UV and HPLC are also known for analysis [16].…”

Photoisomerization Kinetics of Cefuroxime Axetil and Related Compounds

“…Key:( 8 ) isomer B syn; (0) isomer A syn, (e) isomer €3 anti, (0) isomer A antt (W) isomer B syn kept in the dark; ( 0 ) isomer A syn kept in the dark.respective couples of diastereomers for I and I1 were separated. Typical capacity factors were 6.1 (Id, 5.1 (Id, 13.1 (IIA),10.6 (IIB), 6.9 (I& and I&), and 0.9 (IV).The linearity of the calibration graphs (height-concentration) was assessed by injecting six mixed standard solutions ranging between 2 X 10-4 and 2 x 10-5 M for each compound. The graph was linear (r > 0.999) and went through the origin (p = 0.05) in all cases.The repeatability of the chromatographic system was assessed at two different concentrations (2 X 10" and 2 X 1W M).…”

Farmacocinética da cefuroxima após regime de dose múltipla para antibioticoprofilaxia de pacientes submetidos a cirurgia cardíaca com circulação extracorpórea

“…(SALEM e ASKAL, 2002) e a espectrofotometria (absorção atômica, absorção no visível (MAHMOUD et al, 2009;AMIN e RAGABB, 2004;AYAD et al, 1999;OKOYE et al, 2007) além da eletroforese capilar (SCIACCHITANO et al, 1994;PAJCHEL & TYSKI, 2000), e da cromatografia líquida de alta eficiência (COOMBER et al, 1982;ZIVANOVIC et at., 2004;RODENBACH et al, 2006).…”